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831.
Parth Sarthi Sen Gupta Shyamashree Banerjee Rifat Nawaz Ul Islam Sudipta Mondal Buddhadev Mondal Amal K Bandyopadhyay 《Bioinformation》2014,10(2):105-107
In the genomic and proteomic era, efficient and automated analyses of sequence properties of protein have become an important
task in bioinformatics. There are general public licensed (GPL) software tools to perform a part of the job. However, computations
of mean properties of large number of orthologous sequences are not possible from the above mentioned GPL sets. Further, there is
no GPL software or server which can calculate window dependent sequence properties for a large number of sequences in a single
run. With a view to overcome above limitations, we have developed a standalone procedure i.e. PHYSICO, which performs various
stages of computation in a single run based on the type of input provided either in RAW-FASTA or BLOCK-FASTA format and
makes excel output for: a) Composition, Class composition, Mean molecular weight, Isoelectic point, Aliphatic index and GRAVY,
b) column based compositions, variability and difference matrix, c) 25 kinds of window dependent sequence properties. The
program is fast, efficient, error free and user friendly. Calculation of mean and standard deviation of homologous sequences sets,
for comparison purpose when relevant, is another attribute of the program; a property seldom seen in existing GPL softwares.
Availability
PHYSICO is freely available for non-commercial/academic user in formal request to the corresponding author ni.ca.vinurub.hcetoib@eejrenabka 相似文献832.
Ahmed K. Al-Delaimy Hesham M. Al-Mekhlafi Nabil A. Nasr Hany Sady Wahib M. Atroosh Mohammed Nashiry Tengku S. Anuar Norhayati Moktar Yvonne A. L. Lim Rohela Mahmud 《PLoS neglected tropical diseases》2014,8(8)
Background
This cross-sectional study aimed to investigate the current prevalence and risk factors associated with intestinal polyparasitism (the concurrent infection with multiple intestinal parasite species) among Orang Asli school children in the Lipis district of Pahang state, Malaysia.Methods/Principal findings
Fecal samples were collected from 498 school children (50.6% boys and 49.4% girls), and examined by using direct smear, formalin-ether sedimentation, trichrome stain, modified Ziehl Neelsen stain, Kato-Katz, and Harada Mori techniques. Demographic, socioeconomic, environmental, and personal hygiene information were collected by using a pre-tested questionnaire. Overall, 98.4% of the children were found to be infected by at least one parasite species. Of these, 71.4% had polyparasitism. The overall prevalence of Trichuris trichiura, Ascaris lumbricoides, hookworm, Giardia duodenalis, Entamoeba spp., and Cryptosporidium spp. infections were 95.6%, 47.8%, 28.3%, 28.3%, 14.1% and 5.2%, respectively. Univariate and multivariate analyses showed that using an unsafe water supply as a source for drinking water, presence of other family members infected with intestinal parasitic infections (IPI), not washing vegetables before consumption, absence of a toilet in the house, not wearing shoes when outside, not cutting nails periodically, and not washing hands before eating were significant risk factors associated with intestinal polyparasitism among these children.Conclusions/Significance
Intestinal polyparasitism is highly prevalent among children in the peninsular Malaysian Aboriginal communities. Hence, effective and sustainable control measures, including school-based periodic chemotherapy, providing adequate health education focused on good personal hygiene practices and proper sanitation, as well as safe drinking water supply should be implemented to reduce the prevalence and consequences of these infections in this population. 相似文献833.
834.
Li X Molina-Molina M Abdul-Hafez A Ramirez J Serrano-Mollar A Xaubet A Uhal BD 《American journal of physiology. Lung cellular and molecular physiology》2006,291(5):L887-L895
Previous work from this laboratory demonstrated de novo synthesis of angiotensin (ANG) peptides by apoptotic pulmonary alveolar epithelial cells (AEC) and by lung myofibroblasts in vitro and in bleomycin-treated rats. To determine whether these same cell types also synthesize ANG peptides de novo within the fibrotic human lung in situ, we subjected paraffin sections of normal and fibrotic (idiopathic pulmonary fibrosis, IPF) human lung to immunohistochemistry (IHC) and in situ hybridization to detect ANG peptides and angiotensinogen (AGT) mRNA. These were analyzed both alone and in combination with cell-specific markers of AEC [monoclonal antibody (MAb) MNF-116] and myofibroblasts [alpha-smooth muscle actin (alpha-SMA) MAb] and an in situ DNA end labeling (ISEL) method to detect apoptosis. In normal human lung, IHC detected AGT protein in smooth muscle underlying normal bronchi and vessels, but not elsewhere. Real-time RT-PCR and Western blotting revealed that AGT mRNA and protein were 21-fold and 3.6-fold more abundant, respectively, in IPF lung biopsies relative to biopsies of normal human lung (both P < 0.05). In IPF lung, both AGT protein and mRNA were detected in AEC that double-labeled with MAb MNF-116 and with ISEL, suggesting AGT expression by apoptotic epithelia in situ. AGT protein and mRNA also colocalized to myofibroblast foci detected by alpha-SMA MAb, but AGT mRNA was not detected in smooth muscle. These data are consistent with earlier data from isolated human lung cells in vitro and bleomycin-induced rat lung fibrosis models, and they suggest that apoptotic AEC and myofibroblasts constitute key sources of locally derived ANG peptides in the IPF lung. 相似文献
835.
Siddig A. Rahoud Adil Mergani Ammar H. Khamis Osman K. Saeed Qurashi Mohamed-Ali Alain J. Dessein & Nasr Eldin M.A. Elwali 《FEMS immunology and medical microbiology》2010,58(1):106-112
An association study of a cohort of 177 Sudanese patients infected with Schistosoma mansoni [82 (46%) males and 95 (54%) females] was conducted to evaluate the factors controlling the regression of liver fibrosis 39 months after treatment with praziquantel using ultrasound evaluation. Periportal fibrosis (PPF) was regressed in 63 (35.6%) patients, while the disease progressed to higher grades in 24 (13.6%) patients. The grade of PPF did not change in 90 (50.8%) patients. The mean values of portal vein diameter, splenic vein diameter and index liver size in subjects in whom PPF regressed after treatment were significantly lower than in subjects in whom the disease was progressed ( P <0.0001, P =0.031 and P =0.003, respectively). The progression of hepatic fibrosis in males (15, 8.5%) was greater than that in females (9, 5.1%). Patients with regression or progression phenotypes tend to cluster in certain families. Our study indicated that regression, progression and stabilization of PPF after praziquantel therapy is controlled by gender, age, grade of fibrosis and possibly inherited factors. 相似文献
836.
Rho HS Ahn SM Lee BC Kim MK Ghimeray AK Jin CW Cho DH 《Bioorganic & medicinal chemistry letters》2010,20(24):7534-7536
The tyrosinase inhibitory activity of ethanolic extract of kenaf (Hibiscus cannabinus L.) leaf was evaluated before and after subjecting it to far-infrared (FIR) irradiation. The main component of the extract was analyzed as kaempferitrin (kaempferol-3,7-O-α-dirhamnoside). Prior to FIR irradiation, no inhibitory activity of the extract was detected in a tyrosinase assay. However, after FIR irradiation for 1h at 60°C, significant tyrosinase inhibitory activity (IC(50)=3500 ppm) was observed in it. In HPLC analysis, derhamnosylation products (kaempferol, afzelin, and α-rhamnoisorobin) were detected. The inhibitory activity may be due to the existence of derhamnosylation products. This study demonstrated that FIR irradiation can be used as a convenient tool for deglycosylation of flavonoid glycoside. 相似文献
837.
Behery FA Elnagar AY Akl MR Wali VB Abuasal B Kaddoumi A Sylvester PW El Sayed KA 《Bioorganic & medicinal chemistry》2010,18(22):8066-8075
Tocotrienols are vitamin E members with potent antiproliferative activity against preneoplastic and neoplastic mammary epithelial cells with little or no effect on normal cell growth or functions. However, physicochemical and pharmacokinetic properties greatly limit their use as therapeutic agents. Tocotrienols' chemical instability, poor water solubility, NPC1L1-mediated transport, and rapid metabolism are examples of such obstacles which hinder the therapeutic use of these valuable natural products. Vitamin E esters like α-tocopheryl succinate were prepared to significantly improve chemical and metabolic stability, water solubility, and potency. Thus, 12 semisynthetic tocotrienol ester analogues 4-15 were prepared by direct esterification of natural tocotrienol isomers with various acid anhydrides or chlorides. Esters 4-15 were evaluated for their ability to inhibit the proliferation and migration of the mammary tumor cells +SA and MDA-MB-231, respectively. Esters 5, 9, and 11 effectively inhibited the proliferation of the highly metastatic +SA rodent mammary epithelial cells with IC(50) values of 0.62, 0.51, and 0.86μM, respectively, at doses that had no effect on immortalized normal mouse CL-S1 mammary epithelial cells. Esters 4, 6, 8-10, and 13 inhibited 50% of the migration of the human metastatic MDA-MB-231 breast cancer cells at a single 5μM dose in wound-healing assay. The most active ester 9 was 1000-fold more water-soluble and chemically stable versus its parent α-tocotrienol (1). These findings strongly suggest that redox-silent tocotrienol esters may provide superior therapeutic forms of tocotrienols for the control of metastatic breast cancer. 相似文献
838.
Amal Wahhab David Smil Alain Ajamian Martin Allan Yves Chantigny Eric Therrien Natalie Nguyen Sukhdev Manku Silvana Leit Jubrail Rahil Andrea J. Petschner Ai-Hua Lu Alina Nicolescu Sylvain Lefebvre Samuel Montcalm Marielle Fournel Theresa P. Yan Zuomei Li Jeffrey M. Besterman Robert Déziel 《Bioorganic & medicinal chemistry letters》2009,19(2):336-340
The sulfamide moiety has been utilized to design novel HDAC inhibitors. The potency and selectivity of these inhibitors were influenced both by the nature of the scaffold, and the capping group. Linear long-chain-based analogs were primarily HDAC6-selective, while analogs based on the lysine scaffold resulted in potent HDAC1 and HDAC6 inhibitors. 相似文献
839.
Martin Allan Sukhdev Manku Eric Therrien Natalie Nguyen Sylvia Styhler Marie-France Robert Anne-Christine Goulet Andrea J. Petschner Gabi Rahil A. Robert MacLeod Robert Déziel Jeffrey M. Besterman Hannah Nguyen Amal Wahhab 《Bioorganic & medicinal chemistry letters》2009,19(4):1218-1223
A series of N-benzyl-1-heteroaryl-3-(trifluoromethyl)-1H-pyrazole-5-carboxamides targeting co-activator associated arginine methyltransferase 1 (CARM1) have been designed and synthesized. The potency of these inhibitors was influenced by the nature of the heteroaryl fragment with the thiophene analogues being superior to thiazole, pyridine, isoindoline and benzofuran based inhibitors. 相似文献
840.
This study was designed to investigate the susceptibility of liver and brain tissues, as insulin-independent tissues, of normal
adult male rats to the oxidative challenge of subchronic supplementation with chromium picolinate (CrPic) at low (human equivalent)
and high doses (2.90 and 13.20 μg Cr kg−1 day−1, respectively). Also, the modulative effect of CrPic administration on the enhanced oxidative stress in the liver and brain
tissues of alloxan-diabetic rats was studied. Fasting serum glucose level was not modified in normal rats but significantly
reduced in diabetic rats that had received CrPic supplement. A mild oxidative stress was observed in the liver and brain of
CrPic-supplemented normal rats confirmed by the dose-dependent reductions in the levels of hepatic and cerebral free fatty
acids, superoxide dismutase and glutathione peroxidase activities, and in contrast increased tissue malondialdehyde concentration.
On the other hand, hepatic and cerebral catalase activity was reduced in the high dose group only. CrPic supplementation did
not act as a peroxisome proliferator confirmed by the significant reductions in liver and brain peroxisomal palmitoyl CoA
oxidase activity. The non significant alterations in liver protein/DNA and RNA/DNA ratios indicate that CrPic did not affect
protein synthesis per cell, and that mild elevations in hepatic total protein and RNA concentrations might be due to block
or decrease in the export rate of synthesized proteins from the liver to the plasma. In diabetic rats, elevated levels of
hepatic and cerebral free fatty acids and malondialdehyde, and in contrast the overwhelmed antioxidant enzymes, were significantly
modulated in the low dose group and near-normalized in the high dose group. The significant increases observed in liver total
protein and RNA concentrations, as well as protein/DNA and RNA/DNA ratios in diabetic rats supplemented with the high dose
of Cr, compared to untreated diabetics, may be related to the improvement in the glycemic status of the diabetic animals rather
than the direct effect of CrPic on protein anabolism. 相似文献