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281.
Loss in microbial diversity affects nitrogen cycling in soil 总被引:3,自引:0,他引:3
Laurent Philippot Aymé Spor Catherine Hénault David Bru Florian Bizouard Christopher M Jones Amadou Sarr Pierre-Alain Maron 《The ISME journal》2013,7(8):1609-1619
Microbial communities have a central role in ecosystem processes by driving the Earth''s biogeochemical cycles. However, the importance of microbial diversity for ecosystem functioning is still debated. Here, we experimentally manipulated the soil microbial community using a dilution approach to analyze the functional consequences of diversity loss. A trait-centered approach was embraced using the denitrifiers as model guild due to their role in nitrogen cycling, a major ecosystem service. How various diversity metrics related to richness, eveness and phylogenetic diversity of the soil denitrifier community were affected by the removal experiment was assessed by 454 sequencing. As expected, the diversity metrics indicated a decrease in diversity in the 1/103 and 1/105 dilution treatments compared with the undiluted one. However, the extent of dilution and the corresponding reduction in diversity were not commensurate, as a dilution of five orders of magnitude resulted in a 75% decrease in estimated richness. This reduction in denitrifier diversity resulted in a significantly lower potential denitrification activity in soil of up to 4–5 folds. Addition of wheat residues significantly increased differences in potential denitrification between diversity levels, indicating that the resource level can influence the shape of the microbial diversity–functioning relationship. This study shows that microbial diversity loss can alter terrestrial ecosystem processes, which suggests that the importance of functional redundancy in soil microbial communities has been overstated. 相似文献
282.
Alain Senghor K. Ngute Bonaventure Sonké Moses Nsanyi Sainge Kim Calders Rob Marchant Aida Cuni-Sanchez 《African Journal of Ecology》2020,58(3):503-513
Tropical montane forests can store and sequester substantial amounts of carbon in above-ground biomass (AGB), but variations in this storage related to location or degradation have not been quantified in the Cameroon Highlands. We established 25 permanent plots (20 m × 40 m) and sampled all trees ≥10 cm diameter following standard RAINFOR protocols. We estimated AGB and investigated variations related to taxonomic and structural forest attributes, including the height–diameter allometry in five forest types (four old-growth dominated by different species and one secondary forest). Secondary forests had significantly lower AGB than old-growth forests (49.4 ± 2.5 vs. >476.3 ± 168.7 Mg/ha, respectively), mostly related to lower basal area and tree height. Significant differences in species composition but not in forest structure or AGB were found between the four types of old-growth forests studied, located at different altitudes and mountains. We discuss the importance of these montane forests for carbon storage and, considering their high diversity and current threats, their potential for carbon finance mechanisms related to both avoided deforestation and forest restoration. 相似文献
283.
Imen Gargouri Astasselbe Abba Hadja Inna Sylvia Franc Pascale Picaud Alfred Penfornis Coralie Amadou 《Endocrine practice》2022,28(2):185-190
ObjectiveInsulin pump discontinuation has mostly been studied in children and adolescents living with diabetes. We aimed to assess the rate of insulin pump continuation in a population of adult patients with diabetes, at 18 months after initiation; determine the factors associated with pump discontinuation; and develop a simple prediction model.MethodsThis single-center, retrospective study included all adult patients with type 1 diabetes or type 2 diabetes who started insulin pump treatment between January 2015 and June 2018. The exclusion criteria were pregnancy, short-term pregnancy plans, and insulin pump discontinuation within the previous 6 months. The probability of insulin pump continuation after 18 months was estimated using the Kaplan-Meier method. Factors associated with insulin pump discontinuation were studied using a Cox regression model, and an exponential model was built for prediction purposes.ResultsThe study included 315 patients. The mean age was 41 years, the mean duration of diabetes was 16 years, 50% were men, 74% had type 1 diabetes, and the mean hemoglobin A1c level was 9.1% (76 mmol/mol). After 18 months, the rate of insulin pump continuation was 0.80 (95% Confidence Interval (CI), 0.76-0.85). By multivariate analysis, the occurrence of severe hypoglycemia in the previous year was associated with insulin pump discontinuation (hazard ratio, 2.42; 95% CI, 1.30-4.51), while other factors did not reach statistical significance.ConclusionInsulin pump discontinuation occurred in 20% of patients at 18 months after initiation and was mainly associated with a recent history of severe hypoglycemia. The type of diabetes and glycemic control at baseline were not associated with treatment discontinuation. 相似文献
284.
A series of experiments were performed withP. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the β-lactam antibiotics. The constitutive
β-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol.
The strain was also submitted to a series of treatments with Tris and Tris -EDTA to disrupt the outer membrane. The treated
cells demonstrated a ten-fold reduction in the MIC with cloxacillin, six-fold with penicillin, and five-fold with oxacillin.
At least two different biochemical mechanisms were responsible for the resistance to the β-lactams studied which could be
important in the prevalence ofP. aeruginosa in nosocomial infections. 相似文献
285.
Samhita S Rhodes Amadou KS Camara Kristina M Ropella Said H Audi Matthias L Riess Paul S Pagel David F Stowe 《Biomedical engineering online》2006,5(1):16-14
Background
The phase-space relationship between simultaneously measured myoplasmic [Ca2+] and isovolumetric left ventricular pressure (LVP) in guinea pig intact hearts is altered by ischemic and inotropic interventions. Our objective was to mathematically model this phase-space relationship between [Ca2+] and LVP with a focus on the changes in cross-bridge kinetics and myofilament Ca2+ sensitivity responsible for alterations in Ca2+-contraction coupling due to inotropic drugs in the presence and absence of ischemia reperfusion (IR) injury. 相似文献286.
Bruno Senghor Omar Talla Diaw Souleymane Doucoure Mouhamadane Seye Adiouma Diallo Idrissa Talla Cheikh T. Ba Cheikh Sokhna 《PLoS neglected tropical diseases》2016,10(3)
In Sub-Saharan Africa, urogenital schistosomiasis remains a significant public health problem, causing 150.000 deaths/year with approximately 112 million cases diagnosed. The Niakhar district is a disease hotspot in central Senegal where transmission occurs seasonally with high prevalences. The aim of this study was to determine the effect of annual treatment over 3 years on the seasonal transmission dynamics of S. haematobium in 9 villages in the Niakhar district. Adults and children aged between 5 and 60 years were surveyed from 2011 to 2014. Urine samples were collected door-to-door and examined for S. haematobium eggs at baseline in June 2011, and all participants were treated in August 2011 with PZQ (40 mg/kg). After this initial examination, evaluations were conducted at 3 successive time points from September 2011 to March 2014, to measure the efficacy of the annual treatments and the rates of reinfection. Each year, during the transmission period, from July to November-December, malacological surveys were also carried out in the fresh water bodies of each village to evaluate the infestation of the snail intermediate hosts. At baseline, the overall prevalence of S. haematobium infection was 57.7%, and the proportion of heavy infection was 45.3%, but one month after the first treatment high cure rates (92.9%) were obtained. The overall infection prevalence and proportion of heavy infection intensities were drastically reduced to 4.2% and 2.3%, respectively. The level of the first reinfection in February-March 2012 was 9.5%. At follow-up time points, prevalence levels varied slightly between reinfection and treatment from 9.5% in June 2012 to 0.3% in March 2013, 11.2 in June 2013, and 10.1% April 2014. At the end of the study, overall prevalence was significantly reduced from 57.7% to 10.1%. The overall rate of infested Bulinid snails was reduced after repeated treatment from 0.8% in 2012 to 0.5% in 2013. Repeated annual treatments are suggested to have a considerable impact on the transmission dynamics of S. haematobium in Niakhar, due to the nature of the epidemiological system with seasonal transmission. Thus, to maintain this benefit and continue to reduce the morbidity of urogenital schistosomiasis, other approaches should be integrated into the strategy plans of the National program to achieve the goal of urogenital schistosomiasis elimination in seasonal foci in Senegal. 相似文献
287.
Nesrine Lenchi Salima Kebbouche-Gana Pierre Servais Mohamed Lamine Gana Marc Llirós 《Geomicrobiology journal》2020,37(5):454-466
AbstractIn this study, a diesel oil-degrading bacterium was isolated from an oilfield water injection (water-bearing formations, 1,205?m depth) in Algeria. The bacterial strain, designated NL1, was cultivated on diesel oil as sole carbon and energy sources. Molecular analyses of the 16S rRNA gene sequence (KY397882) placed NL1 strain closely related to distinct cultivated species of the Delftia genus. Optimal diesel oil biodegradation by Delftia sp NL1 strain occurred at pH 11, 40?°C, 2?M NaCl and initial hydrocarbon concentration of 5% (v/v) as sole carbon source. GC-MS analyses evidenced that strain Delftia sp NL1 was able to degrade more than 66.76% of diesel oil within only 7?days. On the other hand, and in the same conditions, biosurfactant production by Delftia sp NL1 was also evaluated evidencing high emulsifying capacity (E24 = 81%), ability to lower the surface tension of growing media (with the value of 25.7?mN m?1), and production of glycolipids (8.7?g L?1) as biosurfactants. This research presents indigenous strain Delftia sp NL1 for diesel degradation and synthesis of biosurfactant in extreme conditions. In this sense, strain NL1 is a good candidate for possible in situ oil recovery and in wastewater treatment in refineries and oil terminals in petroleum industry. 相似文献