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171.
Fang Y Banner D Kelvin AA Huang SS Paige CJ Corfe SA Kane KP Bleackley RC Rowe T Leon AJ Kelvin DJ 《Journal of virology》2012,86(4):2229-2238
During the 2009 H1N1 influenza virus pandemic (pdmH1N1) outbreak, it was found that most individuals lacked antibodies against the new pdmH1N1 virus, and only the elderly showed anti-hemagglutinin (anti-HA) antibodies that were cross-reactive with the new strains. Different studies have demonstrated that prior contact with the virus can confer protection against strains with some degree of dissimilarity; however, this has not been sufficiently explored within the context of a pdmH1N1 virus infection. In this study, we have found that a first infection with the A/Brisbane/59/2007 virus strain confers heterologous protection in ferrets and mice against a subsequent pdmH1N1 (A/Mexico/4108/2009) virus infection through a cross-reactive but non-neutralizing antibody mechanism. Heterologous immunity is abrogated in B cell-deficient mice but maintained in CD8(-/-) and perforin-1(-/-) mice. We identified cross-reactive antibodies from A/Brisbane/59/2007 sera that recognize non-HA epitopes in pdmH1N1 virus. Passive serum transfer showed that cross-reactive sH1N1-induced antibodies conferred protection in naive recipient mice during pdmH1N1 virus challenge. The presence or absence of anti-HA antibodies, therefore, is not the sole indicator of the effectiveness of protective cross-reactive antibody immunity. Measurement of additional antibody repertoires targeting the non-HA antigens of influenza virus should be taken into consideration in assessing protection and immunization strategies. We propose that preexisting cross-protective non-HA antibody immunity may have had an overall protective effect during the 2009 pdmH1N1 outbreak, thereby reducing disease severity in human infections. 相似文献
172.
173.
Benoit JB Jajack AJ Yoder JA 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2012,182(2):189-198
To examine how traumatic insemination, a wounding process to females inflicted by males during copulation, reduces the longevity
of females of the bedbug, Cimex
lectularius, we assessed if multiple bouts of mating impact water relations of females by measuring net transpiration water loss rates.
Our studies show that net transpiration rate of females correlates with frequency of mating (small increase after exposure
to low numbers of males; large increase after exposure to large numbers of males), and this is reflected by reduced female
survivorship for as much as 22 days at 75% RH, 25°C. Water loss occurs up to 28% more rapidly in females after being held
with large groups of males. Females that were exposed to males having their paramere removed, females exposed only to other
females, and females kept in isolation (unmated) exhibited no reduction in ability to retain water, indicating that traumatic
insemination was responsible for the net transpiration rate increase. Mechanical piercing of the female’s abdominal wall leads
to increased net transpiration rates for longer periods than puncturing the ectospermalege (regular mating site), implying
that inaccurate copulation by males is extremely detrimental to the water balance of females and that the ectospermalege is
uniquely modified to seal off more quickly to prevent excess water loss. Mating frequency and the associated increased water
loss is considerably reduced by the addition of bed bug alarm pheromone components. Thus, females experience elevated water
stress due to traumatic insemination, especially at high levels and when males fail to pierce the ectospermalege, and water
loss prevention, likely by more rapid sealing of the wound, is a novel function of the ectopsermalege. 相似文献
174.
Jay A. Yoder Michael J. Chambers Michael R. Condon Joshua B. Benoit Lawrence W. Zettler 《Symbiosis (Philadelphia, Pa.)》2009,47(2):93-98
The giant Madagascar hissing-cockroach,Gromphadorhina portentosa, and its mite associate,Gromphadorholaelaps schaeferi, constitute an intimate commensalistic symbiosis. While the mite’s very survival is dependent by feeding on cockroach saliva
and associated organic debris, the degree that the cockroach benefits from this association is unclear. We investigated the
mite’s potential role at regulating surface fungi on the exoskeletons of this insect. Numbers of fungal isolates that resulted
were compared between captive-bred cockroaches with and without mites. The mycoflora of both groups consisted of common molds
(Alternaria sp.,Aspergillus sp.,Cladosporium sp.,Geotrichum sp.,Mucor sp.,Penicillium sp.,Rhizopus sp.,Trichoderma sp.). The presence of mites reduced the number of isolates by 1/2 in mature females, 1/3 in males, and 1/4 in sixth (final)
instar nymphs. Fungus levels continued to drop when mite-free cockroaches were artificially supplemented with mites. A direct
correlation was detected between mites and the reduction in the quantity of surface molds up to 20 mites per cockroach. The
addition of more mites above 20 per cockroach, even 4x more, had a minimal, but still reducing, effect. Mites regulated all
types of fungi, not just a select few taxa. We propose that mites reduce the mycoflora not because they consume fungi, but
because mites and molds compete for the same resources in an ecological niche, saliva and organic debris that accumulate in
between cockroach’s legs. Cockroaches reared in captivity do not apparently benefit by the removal of surface molds by mites,
lending support for a commensalistic symbiosis. This cockroach species has been linked to severe allergic reactions in children,
in part, because it harbors antagonistic molds. GivenG. schaeferi’s regulatory role at suppressing fungi, these mites could conceivably impose a small indirect, albeit beneficial role to humans
by reducing the amount of fungal inoculum (conidia) that might otherwise be inhaled. 相似文献
175.
We report the development of 18, single-copy, anonymous nuclear loci from the Malagasy plated lizard Zonosaurus madagascariensis. More than 140 clones from a genomic library were examined and 38 potential loci tested across both closely and distantly related lizards. Of the 18 loci reported here, more than half (10) work in closely related zonosaurines although only one successfully amplified a homologous fragment in the distantly related iguanid (Oplurus). Sequences of these loci revealed a high frequency of single nucleotide polymorphisms, supporting previous reports of high levels of intraspecific variation in lizards. 相似文献
176.
Binding of the HIV-1 envelope to its chemokine coreceptors mediates two major biological events: membrane fusion and signaling transduction. The fusion process has been well studied, yet the role of chemokine coreceptor signaling in viral infection has remained elusive through the past decade. With the recent demonstration of the signaling requirement for HIV latent infection of resting CD4 T cells, the issue of coreceptor signaling needs to be thoroughly revisited. It is likely that virus-mediated signaling events may facilitate infection in various immunologic settings in vivo where cellular conditions need to be primed; in other words, HIV may exploit the chemokine signaling network shared among immune cells to gain access to downstream cellular components, which can then serve as effective tools to break cellular barriers. This virus-hijacked aberrant signaling process may in turn facilitate pathogenesis. In this review, we summarize past and present studies on HIV coreceptor signaling. We also discuss possible roles of coreceptor signaling in facilitating viral infection and pathogenesis. 相似文献
177.
178.
Hymenoptera is an extraordinarily diverse lineage, both in terms of species numbers and morphotypes, that includes sawflies, bees, wasps, and ants. These organisms serve critical roles as herbivores, predators, parasitoids, and pollinators, with several species functioning as models for agricultural, behavioral, and genomic research. The collective anatomical knowledge of these insects, however, has been described or referred to by labels derived from numerous, partially overlapping lexicons. The resulting corpus of information--millions of statements about hymenopteran phenotypes--remains inaccessible due to language discrepancies. The Hymenoptera Anatomy Ontology (HAO) was developed to surmount this challenge and to aid future communication related to hymenopteran anatomy. The HAO was built using newly developed interfaces within mx, a Web-based, open source software package, that enables collaborators to simultaneously contribute to an ontology. Over twenty people contributed to the development of this ontology by adding terms, genus differentia, references, images, relationships, and annotations. The database interface returns an Open Biomedical Ontology (OBO) formatted version of the ontology and includes mechanisms for extracting candidate data and for publishing a searchable ontology to the Web. The application tools are subject-agnostic and may be used by others initiating and developing ontologies. The present core HAO data constitute 2,111 concepts, 6,977 terms (labels for concepts), 3,152 relations, 4,361 sensus (links between terms, concepts, and references) and over 6,000 text and graphical annotations. The HAO is rooted with the Common Anatomy Reference Ontology (CARO), in order to facilitate interoperability with and future alignment to other anatomy ontologies, and is available through the OBO Foundry ontology repository and BioPortal. The HAO provides a foundation through which connections between genomic, evolutionary developmental biology, phylogenetic, taxonomic, and morphological research can be actualized. Inherent mechanisms for feedback and content delivery demonstrate the effectiveness of remote, collaborative ontology development and facilitate future refinement of the HAO. 相似文献
179.
180.
Functional analysis of all nonribosomal peptide synthetases in Cochliobolus heterostrophus reveals a factor, NPS6, involved in virulence and resistance to oxidative stress 总被引:1,自引:0,他引:1 下载免费PDF全文
Nonribosomal peptides, made by nonribosomal peptide synthetases, have diverse biological activities, including roles as fungal virulence effectors. Inspection of the genome of Cochliobolus heterostrophus, a fungal pathogen of maize and a member of a genus noted for secondary metabolite production, revealed eight multimodular nonribosomal peptide synthase (NPS) genes and three monomodular NPS-like genes, one of which encodes a nonribosomal peptide synthetase/polyketide synthase hybrid enzyme presumed to be involved in synthesis of a peptide/polyketide molecule. Deletion of each NPS gene and phenotypic analyses showed that the product of only one of these genes, NPS6, is required for normal virulence on maize. NPS6 is also required for resistance to hydrogen peroxide, suggesting it may protect the fungus from oxidative stress. This and all other nps mutants had normal growth, mating ability, and appressoria. Real-time PCR analysis showed that expression of all NPS genes is low (relative to that of actin), that all (except possibly NPS2) are expressed during vegetative growth, and that expression is induced by nitrogen starvation. Only NPS6 is unfailingly conserved among euascomycete fungi, including plant and human pathogens and saprobes, suggesting the possibility that NPS6 activity provides oxidative stress protection during both saprobic and parasitic growth. 相似文献