首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   758248篇
  免费   81992篇
  国内免费   358篇
  840598篇
  2018年   6488篇
  2016年   8574篇
  2015年   11523篇
  2014年   13451篇
  2013年   20149篇
  2012年   22144篇
  2011年   22708篇
  2010年   15296篇
  2009年   14167篇
  2008年   20397篇
  2007年   21074篇
  2006年   20089篇
  2005年   19035篇
  2004年   19143篇
  2003年   18444篇
  2002年   18102篇
  2001年   32037篇
  2000年   32131篇
  1999年   26043篇
  1998年   9441篇
  1997年   9981篇
  1996年   9556篇
  1995年   8802篇
  1994年   8828篇
  1993年   8737篇
  1992年   22459篇
  1991年   21842篇
  1990年   21637篇
  1989年   21553篇
  1988年   19971篇
  1987年   18935篇
  1986年   17660篇
  1985年   18119篇
  1984年   14981篇
  1983年   12946篇
  1982年   9928篇
  1981年   8877篇
  1980年   8438篇
  1979年   14318篇
  1978年   11201篇
  1977年   10354篇
  1976年   9925篇
  1975年   10781篇
  1974年   11572篇
  1973年   11282篇
  1972年   10439篇
  1971年   9384篇
  1970年   8169篇
  1969年   7862篇
  1968年   7254篇
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
81.
Electron microscopy of the adrenal medulla and cortex during the intermediate period of endotoxin shock has revealed severe destructive changes in parenchymal cells. These changes are the reason for synthetic, secretory and trophic disturbances of glandular functions. Ultrastructural lesions indicate that stress exposure exceeds the adaptive capacity of cells, the majority of which are exhausted and killed.  相似文献   
82.
OBJECTIVE--To test the hypothesis that farmers are at high risk of hip osteoarthritis and to investigate possible causes for such a hazard. DESIGN--Cross sectional survey. SETTING--Five rural general practices. SUBJECTS--167 male farmers aged 60-76 and 83 controls from mainly sedentary jobs. All those without previous hip replacement underwent radiography of the hip. MAIN OUTCOME MEASURES--Hip replacement for osteoarthritis or radiological evidence of hip osteoarthritis. RESULTS--Prevalence of hip osteoarthritis was higher in farmers than controls and especially in those who had farmed for over 10 years (odds ratio 9.3, 95% confidence interval 1.9 to 44.5). The excess could not be attributed to any one type of farming, and heavy lifting seems the likely explanation. CONCLUSIONS--Manual handling in agriculture should be limited where possible. Consideration should be given to making hip osteoarthritis a prescribed industrial disease in farmers. There may be wider implications for the prevention of hip osteoarthritis in the general population.  相似文献   
83.
84.
Synthesis of prenylquinones in chloroplasts   总被引:3,自引:0,他引:3  
  相似文献   
85.
Growth of fathead minnow yolk sac larvae was characterized from changes in dry weight and total content and concentrations of RNA, DNA and protein in fish exposed to a sublethal level of HCN (58 micrograms/l) and in age matched controls. Cyanide toxicosis occurred within 24 hr of exposure as evidenced by significant reductions in protein and RNA content and RNA/DNA ratio of larvae. After 96 hr exposure to HCN, larvae exhibited the same growth rate and protein synthetic rate (RNA/DNA) as control fish. HCN toxicosis and recovery is rapid and at least partial tolerance to HCN develops within 96 hr of exposure in larval fathead minnows.  相似文献   
86.
87.
G R Otten  M R Loken 《Cytometry》1982,3(3):182-187
Forward angle light scattering of two different wavelengths by cells in a flow cytometer was used to investigate physical differences between lymphocytes of different lineage, functional subclass and developmental stage. Correlation of the ultraviolet (UV: 351 nm and 364 nm) and 488 nm light scattering signals produced by lymphoid cells demonstrated that the two signals were not equivalent and that they placed different emphasis on the physical parameters characterizing lymphocytes. Both small T and B lymphocytes from peripheral lymphoid tissues and mitogenically activated large T and B lymphocyte blasts were discriminated by both wavelengths. Differences between the Lyt-2 negative and Lyt-2 positive T lymphocyte subsets were also apparent. Two color light scattering could also discriminate between immature thymocytes and mature peripheral T cells and between small bone marrow cells and mature peripheral B cells. In bone marrow an increase in UV light scattering coincided with the appearance of cell surface immunoglobulin on small cells. These data establish that two color light scattering is a sensitive probe for distinguishing cells of apparently similar morphology and that it can be used to study the physical changes that occur during lymphoid cell differentiation.  相似文献   
88.
89.
A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria. Specific activities of two marker enzymes, amylase and chymotrypsin, are increased by 4.6 and 5.4-fold, respectively, as compared to the homogenate. The purified fraction is devoid of detectable RNA, DNA and 5'-nucleotidase, glucose-6-phosphatase, and cytochrome c oxidase activities. Electron micrographs confirm the absence of mitochondria, lysosomes, and rough endoplasmic reticulum fragments. Zymogen granule membranes were isolated from this fraction on a sucrose gradient following lysis in alkaline buffer. Secretory contaminants were efficiently removed from the membranes as indicated by experiments in which labeled secretory proteins were added during the isolation procedure and secondly by measuring residual levels of amylase and chymotrypsin. Three enzyme activities were found in the membranes: thiamine pyrophosphatase, ATP-diphosphohydrolase, and low levels of acid phosphatase. Membrane proteins were solubilized by urea-Triton X-100 and separated in double-dimension (isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Isoelectric point and molecular weight of each protein band were determined.  相似文献   
90.
The Thom gradient model of morphogenesis poses the followinga posteriori problem: “From the observed morphology of a given natural process (effect) determine the dynamics of the process (cause)”. In this paper we consider the classicala priori problem: “Given the cause (dynamics) determine the effect (resultant morphology)”. We find that in biochemical processes the mechanisms for energy activation, energy-matter interaction and energy dissipation determine the dynamics. Furthermore there exists basic energy mechanisms which drive the equilibrium states through the elementary catastrophes of Thom. A comparison with current theories shows that our models describe open ecological food chains and their dynamical systems generalize the equations of organisation posed by M. Eigen. Work supported by a Research Associateship of the International Centre for Theoretical Physics, P.O.B. 586, Miramare, 34100 Trieste, Italy.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号