全文获取类型
收费全文 | 4472篇 |
免费 | 446篇 |
国内免费 | 1篇 |
出版年
2022年 | 30篇 |
2021年 | 57篇 |
2020年 | 39篇 |
2019年 | 61篇 |
2018年 | 63篇 |
2017年 | 49篇 |
2016年 | 96篇 |
2015年 | 150篇 |
2014年 | 143篇 |
2013年 | 234篇 |
2012年 | 257篇 |
2011年 | 245篇 |
2010年 | 155篇 |
2009年 | 169篇 |
2008年 | 205篇 |
2007年 | 237篇 |
2006年 | 250篇 |
2005年 | 210篇 |
2004年 | 203篇 |
2003年 | 207篇 |
2002年 | 199篇 |
2001年 | 70篇 |
2000年 | 62篇 |
1999年 | 68篇 |
1998年 | 60篇 |
1997年 | 44篇 |
1996年 | 54篇 |
1995年 | 33篇 |
1994年 | 54篇 |
1993年 | 59篇 |
1992年 | 54篇 |
1991年 | 56篇 |
1990年 | 52篇 |
1989年 | 39篇 |
1988年 | 48篇 |
1987年 | 53篇 |
1986年 | 28篇 |
1985年 | 43篇 |
1984年 | 53篇 |
1983年 | 42篇 |
1982年 | 46篇 |
1981年 | 48篇 |
1980年 | 39篇 |
1979年 | 28篇 |
1978年 | 39篇 |
1977年 | 42篇 |
1976年 | 27篇 |
1975年 | 27篇 |
1974年 | 29篇 |
1972年 | 28篇 |
排序方式: 共有4919条查询结果,搜索用时 31 毫秒
11.
Michael F. Hammer James W. Schilling Ellen M. Prager Allan C. Wilson 《Journal of molecular evolution》1987,24(3):272-279
Summary Two major types of lysozymec (M and P) occur in the mouse genus,Mus, and have been purified from an inbred laboratory strain (C58/J) ofM. domesticus. They differ in physical, catalytic, and antigenic properties as well as by amino acid replacements at 6 of 49 positions in the amino-terminal sequence. Comparisons with four other mammalian lysozymesc of known sequence suggest that M and P are related by a gene duplication that took place before the divergence of the rat and mouse lineages. M lysozyme is present in most tissues; achieves its highest concentration in the kidney, lung, and spleen; and corresponds to the lysozyme partially sequenced before from another strain ofM. domesticus. InM. domesticus and several related species, P lysozyme was detected chiefly in the small intestine, where it is probably produced mainly by Paneth cells. A survey of M and P levels in 22 species of muroid rodents (fromMus and six other genera) of known phylogenetic relationships suggests that a mutation that derepressed the P enzyme arose about 4 million years ago in the ancestor of the housemouse group of species. Additional regulatory shifts affecting M and P levels have taken place along lineages leading to other muroid species. Our survey of 187 individuals of wild house mice and their closest allies reveals a correlation between latitude of origin and level of intestinal lysozyme. 相似文献
12.
Mitochondrial DNA of the extinct quagga: Relatedness and extent of postmortem change 总被引:4,自引:0,他引:4
Russell G. Higuchi Lisa A. Wrischnik Elizabeth Oakes Matthew George Benton Tong Allan C. Wilson 《Journal of molecular evolution》1987,25(4):283-287
Sequences are reported for portions of two mitochondrial genes from a domestic horse and a plains zebra and compared to those published for a quagga and a mountain zebra. The extinct quagga and plains zebra sequences are identical at all silent sites, whereas the horse sequence differs from both of them by 11 silent substitutions. Postmortem changes in quagga DNA may account for the two coding substitutions between the quagga and plains zebra sequences. The hypothesis that the closest relative of the quagga is the domestic horse receives no support from these data. From the extent of sequence divergence between horse and zebra mitochondrial DNAs (mtDNAs), as well as from information about the fossil record, we estimate that the mean rate of mtDNA divergence in Equus is similar to that in other mammals, i.e., roughly 2% per million years. 相似文献
13.
The purpose of this work was to study the genetic basis of histocompatibility antigens encoded by the mouse minor histocompatibility
(H) locusH-3. Both class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) and class II MHC-restricted
helper T cells (TH) specific for antigens encoded by genes within theH-3 locus were isolated and analyzed. Typing a number of mouse strains for expression of antigens recognized by these TH and
CTL suggested that there was a different strain distribution pattern of expression of the antigens recognized by TH compared
with those recognized by CTL. Separation of the genes whose products stimulate TH from those whose products stimulate CTL
was suggested by: (1) analysis of the strain B10.FS(92NX)/Grf that has undergone recombination within theH-3 region; (2) genetic segregation studies of (B10.UW-H-3
b/Sn×C57BL/10Sn)F2 mice; and (3) F1 complementation studies in which CTL specific for products of the TH-defined gene(s) could not be detected, even in the absence
of immune responses to products of the CTL-defined genes. Taken together, these data suggest that in addition to two genes
(B2m andCd-1) within theH-3 region whose products typically stimulate class I MHC-restricted CTL, there is at least one additional gene whose product
selectively stimulates class II MHC-restricted TH. This new gene is located telomeric from the CTL-defined genes and between
the lociwe andun on chromosome 2. These data demonstrate a novel degree of complexity of theH-3 “locus” and suggest selective presentation of minor H gene products in the context of class I or class II MHC proteins. 相似文献
14.
The ras oncogene and tumour metastasis: observations on murine cells transfected with activated human c-Ha-ras 总被引:1,自引:0,他引:1
Jeannie S. Wallace Allan J. Hayle Allan J. Syms Margaret Cairney Ben Tutty rew Gazzard Mark F. Evans Kenneth A. Fleming David Tarin 《Differentiation; research in biological diversity》1989,41(3):208-215
Transfection of cells with cloned genes or total genomic DNA offers a means for studying aspects of neoplastic behaviour. We have used this method to examine whether incorporation of the cloned 6.6-kilobase (kb) fragment of DNA containing the mutant c-Ha-ras human oncogene can confer metastatic capability on murine NIH 3T3 cells. Cells co-transfected with the mutated ras gene and the neomycin resistance marker pSV2neo were selected by culture in neomycin. On subcutaneous inoculation into MF 1 nude mice, these cells proved to be tumourigenic with short latent periods (approximately 14 days)--nude mice were used to circumvent immunological rejection of the mouse cells expressing the product of the human oncogene. Transfectants were capable of lung colonisation after intravenous injection, but there was no evidence of spontaneous metastasis at autopsy, or on histological examination of the lungs and other organs, 90 days after inoculation. Incorporation of the transfected oncogene was confirmed by Southern blotting and its expression by dot-blot hybridisation and immunoprecipitation. The results in this experimental system indicate that transfection of a mutated human ras oncogene into non-neoplastic 3T3 cells can confer part of the metastatic phenotype, namely lung colonisation, but is not by itself sufficient to induce spontaneous metastatic behaviour. 相似文献
15.
Candida albicans is an opportunistic pathogen of human mucosal surfaces. Colonization of oral and vaginal mucosa by this yeast is antagonized by the resident normal bacterial population. However, antibacterial therapy can alter the normal flora to allow fungal cells to attach, grow and invade host tissues. We studied the antimicrobic activity of fluconazole against clinical isolates of oral and vaginal bacteria and Candida albicans in vitro and in vivo by scanning and transmission electron microscopy; we also compared the bactericidal activity of fluconazole with clotrimazole in vitro by microbiologie assay. Fluconazole lysed fungi but did not change the ultrastructure of bacteria. Clotrimazole, but not fluconazole, was bactericidal against lactobacillus and streptococcus, the principal species of the oral and vaginal cavities. We conclude that Candida albicans, but not oral and vaginal bacteria, is susceptible to fluconazole. These observations help explain the antimycotic specificity of fluconazole and its efficacy against candidiasis in humans. 相似文献
16.
Reclustering of scattered Golgi elements occurs along microtubules 总被引:43,自引:0,他引:43
W C Ho V J Allan G van Meer E G Berger T E Kreis 《European journal of cell biology》1989,48(2):250-263
Depolymerization of the interphase microtubules by nocodazole results in the scattering and apparent fragmentation of the Golgi apparatus in Vero fibroblast cells. Upon removal of the drug, the interphase microtubules repolymerize, and the scattered Golgi elements move back to the region around the microtubule-organizing center (MTOC) within 40 to 60 min. Using a fluorescent lipid analogue (C6-NBD-ceramide) as a vital stain for the scattered Golgi elements, their relocation was visualized by video-enhanced fluorescence microscopy in Vero cells maintained at 20 degrees C. The NBD-labeled structures were identified as Golgi elements by their colocalization with galactosyltransferase in the fixed cells. During reclustering, NBD-labeled Golgi elements were observed to move by discontinuous saltations towards the MTOC with velocities of 0.1 to 0.4 micron/s. Paths along which Golgi elements moved were super-imposable on microtubules visualized by indirect immunofluorescence. Neither the collapse of intermediate filaments caused by microinjection of antibodies to vimentin nor the disruption of microfilaments by cytochalasin D had an effect on the reclustering of Golgi elements or the positioning of the Golgi apparatus. These data show that scattered Golgi elements move along microtubules back to the region around the MTOC, while neither intact intermediate filaments nor microfilaments are involved. 相似文献
17.
Isozyme analysis of rodent-human somatic cell hybrids has been used frequently to detect specific human chromosomes. The majority of these isozyme systems employs starch gels, the use of which can be laborious when screening large numbers of cell lines. We describe the development of two procedures to detect the long arms of human chromosomes 1 and 2 in Chinese hamster-human cell hybrids by a rapid and reproducible method using 1-mm-thick agarose gels. Detection of human chromosome 1q was accomplished by screening for human fumarate hydratase activity, whose gene has been mapped to 1q42.1. Detection of chromosome 2q was performed by screening for the isozyme isocitrate dehydrogenase 1, which has been localized to 2q32-qter. These systems provide a basis for the further development of procedures for detecting chromosome-specific isozyme markers in agarose gels. 相似文献
18.
Testosterone, and winning and losing in human competition 总被引:9,自引:4,他引:5
Alan Booth Greg Shelley Allan Mazur Gerry Tharp Roger Kittok 《Hormones and behavior》1989,23(4):556-571
Testosterone and cortisol were measured in six university tennis players across six matches during their varsity season. Testosterone rose just before most matches, and players with the highest prematch testosterone had the most positive improvement in mood before their matches. After matches, mean testosterone rose for winners relative to losers, especially for winners with very positive moods after their victories and who evaluated their own performance highly. Winners with rising testosterone had higher testosterone before their next match, in contrast to losers with falling testosterone, who had lower testosterone before their next match. Cortisol was not related to winning or losing, but it was related to seed (top players having low cortisol), and cortisol generally declined as the season progressed. These results are consistent with a biosocial theory of status. 相似文献
19.
20.
In situ high-temperature, high-pressure Raman experiments on 3 mM (pH 5) aqueous solutions of hen egg-white (HEW) lysozyme show a decrease in the relative height of the 505 cm–1 band associated with S-S stretching vibrations at 72°C (1 bar). The peak height changes are accompanied by significant band broadening, and the integrated band intensity does not change within experimental error. The effect of increased pressure at 72°C was to hinder broadening of the 505 cm–1 band. HEW lysozyme (2.4 mM,pH 5) was also heated at 76°C, 80°C, and 95°C for different periods of time, and aliquots were quenched to room temperature for Raman and enzymatic activity measurements. After 9 hr at 76°C, the protein exhibits enzyme activity less than 50% of the initial value, and approximately 50% reduction in activity is achieved after 3 hr at 80°C or 1 hr at 95°C. The Raman results suggest that different irreversibly denatured conformations are attained during prolonged exposures at these different temperatures. It is apparent from these studies that the S-S stretch intensity is decreased irreversibly. 相似文献