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111.
112.
Functional and structural characterization of chimeras of a bacterial genotoxin and human type I DNAse 总被引:1,自引:0,他引:1
Joseph M. DiRienzo Linsen Cao Alla Volgina Georges Bandelac & Jonathan Korostoff 《FEMS microbiology letters》2009,291(2):222-231
Chimeras composed of the cdtB gene of a novel bacterial genotoxin and the human type I DNAse I gene were constructed and their products characterized relative to the biochemical and enzymatic properties of the native proteins. The product of a cdtB/DNAse I chimera formed a heterotrimer with the CdtA and CdtC subunits of the genotoxin, and targeted mutations increased the specific activity of the hybrid protein. Expression of active chimeric gene products established that the CdtB protein is an atypical divalent cation-dependent endonuclease and demonstrated the potential for genetically engineering a new class of therapeutic agent for inhibiting the proliferation of cancer cells. 相似文献
113.
Investigation of the antifouling constituents from the brown alga Sargassum muticum (Yendo) Fensholt
Alexandra Bazes Alla Silkina Philippe Douzenel Fabienne Faÿ Nelly Kervarec Danièle Morin Jean-Pascal Berge Nathalie Bourgougnon 《Journal of applied phycology》2009,21(4):395-403
One of the most promising alternatives to toxic heavy metal-based paints is offered by the development of antifouling coatings
in which the active ingredients are compounds naturally occurring in marine organisms and operating as natural antisettlement
agents. Sessile marine macroalgae are remarkably free from settlement by fouling organisms. They produce a wide variety of
chemically active metabolites in their surroundings, potentially as an aid to protect themselves against other settling organisms.
In this study, a dichloromethane extract from the brown seaweed Sargassum muticum was tested in situ and, after 2 months of immersion, showed less fouling organisms on paints in which the extract was included,
compared to paints containing only copper after 2 months of immersion. No barnacles or mussels have been observed on the test
rack. Identification by NMR and GC/MS of the effective compound revealed the abundance of palmitic acid, a commonly found
fatty acid. Pure palmitic acid showed antibacterial activity at 44 μg mL−1, and also inhibited the growth of the diatom Cylindrotheca closterium at low concentration (EC50 = 45.5 μg mL−1), and the germination of Ulva lactuca spores at 3 μg mL−1. No cytotoxicity was highlighted, which is promising in the aim of the development of an environmentally friendly antifouling
paint. 相似文献
114.
Clum A Tindall BJ Sikorski J Ivanova N Mavrommatis K Lucas S Glavina T Del Rio Nolan M Chen F Tice H Pitluck S Cheng JF Chertkov O Brettin T Han C Detter JC Kuske C Bruce D Goodwin L Ovchinikova G Pati A Mikhailova N Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Chain P Rohde M Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Lapidus A 《Standards in genomic sciences》2009,1(3):308-316
Pirellula staleyi Schlesner and Hirsch 1987 is the type species of the genus Pirellula of the family Planctomycetaceae. Members of this pear- or teardrop-shaped bacterium show a clearly visible pointed attachment pole and can be distinguished from other Planctomycetes by a lack of true stalks. Strains closely related to the species have been isolated from fresh and brackish water, as well as from hypersaline lakes. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of the order Planctomyces and only the second sequence from the phylum Planctobacteria/Planctomycetes. The 6,196,199 bp long genome with its 4773 protein-coding and 49 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
115.
Pati A Sikorski J Nolan M Lapidus A Copeland A Glavina Del Rio T Lucas S Chen F Tice H Pitluck S Cheng JF Chertkov O Brettin T Han C Detter JC Kuske C Bruce D Goodwin L Chain P D'haeseleer P Chen A Palaniappan K Ivanova N Mavromatis K Mikhailova N Rohde M Tindall BJ Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP 《Standards in genomic sciences》2009,1(2):141-149
Saccharomonospora viridis (Schuurmans et al. 1956) Nonomurea and Ohara 1971 is the type species of the genus Saccharomonospora which belongs to the family Pseudonocardiaceae. S. viridis is of interest because it is a Gram-negative organism classified among the usually Gram-positive actinomycetes. Members of the species are frequently found in hot compost and hay, and its spores can cause farmer's lung disease, bagassosis, and humidifier fever. Strains of the species S. viridis have been found to metabolize the xenobiotic pentachlorophenol (PCP). The strain described in this study has been isolated from peat-bog in Ireland. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first complete genome sequence of the family Pseudonocardiaceae, and the 4,308,349 bp long single replicon genome with its 3906 protein-coding and 64 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
116.
Anderson IJ Sieprawska-Lupa M Lapidus A Nolan M Copeland A Glavina Del Rio T Tice H Dalin E Barry K Saunders E Han C Brettin T Detter JC Bruce D Mikhailova N Pitluck S Hauser L Land M Lucas S Richardson P Whitman WB Kyrpides NC 《Standards in genomic sciences》2009,1(2):189-196
Methanoculleus marisnigri Romesser et al. 1981 is a methanogen belonging to the order Methanomicrobiales within the archaeal phylum Euryarchaeota. The type strain, JR1, was isolated from anoxic sediments of the Black Sea. M. marisnigri is of phylogenetic interest because at the time the sequencing project began only one genome had previously been sequenced from the order Methanomicrobiales. We report here the complete genome sequence of M. marisnigri type strain JR1 and its annotation. This is part of a Joint Genome Institute 2006 Community Sequencing Program to sequence genomes of diverse Archaea. 相似文献
117.
Labutti K Pukall R Steenblock K Glavina Del Rio T Tice H Copeland A Cheng JF Lucas S Chen F Nolan M Bruce D Goodwin L Pitluck S Ivanova N Mavromatis K Ovchinnikova G Pati A Chen A Palaniappan K Land M Hauser L Chang YJ Jeffries CD Chain P Saunders E Brettin T Detter JC Han C Göker M Bristow J Eisen JA Markowitz V Hugenholtz P Kyrpides NC Klenk HP Lapidus A 《Standards in genomic sciences》2009,1(2):159-165
Anaerococcus prevotii (Foubert and Douglas 1948) Ezaki et al. 2001 is the type species of the genus, and is of phylogenetic interest because of its arguable assignment to the provisionally arranged family 'Peptostreptococcaceae'. A. prevotii is an obligate anaerobic coccus, usually arranged in clumps or tetrads. The strain, whose genome is described here, was originally isolated from human plasma; other strains of the species were also isolated from clinical specimen. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of a member of the genus. Next to Finegoldia magna, A. prevotii is only the second species from the family 'Peptostreptococcaceae' for which a complete genome sequence is described. The 1,998,633 bp long genome (chromosome and one plasmid) with its 1852 protein-coding and 61 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project. 相似文献
118.
Yevgeniy Turovskiy Richard D. Ludescher Alla A. Aroutcheva Sebastian Faro Michael L. Chikindas 《Probiotics and antimicrobial proteins》2009,1(1):67-74
Bacterial vaginosis (BV) is a commonly occurring vaginal infection that is associated with a variety of serious risks related to the reproductive health of women. Conventional antibiotic treatment for this condition is frequently ineffective because the antibiotics tend to inhibit healthy vaginal microflora along with the pathogens. Lactocin 160, a bacteriocin produced by healthy vaginal lactobacilli, is a promising alternative to antibiotics; this compound specifically inhibits the BV-associated vaginal pathogens such as Gardnerella vaginalis and Prevotella bivia without affecting the healthy microflora. This study investigates the molecular mechanism of action for lactocin 160 and reveals that this compound targets the cytoplasmic membrane of G. vaginalis, causing the efflux of ATP molecules and dissipation of the proton motive force. 相似文献
119.
Konstantinos Mavromatis Natalia Ivanova Iain Anderson Athanasios Lykidis Sean D. Hooper Hui Sun Victor Kunin Alla Lapidus Philip Hugenholtz Bharat Patel Nikos C. Kyrpides 《PloS one》2009,4(1)
Halothermothirx orenii is a strictly anaerobic thermohalophilic bacterium isolated from sediment of a Tunisian salt lake. It belongs to the order Halanaerobiales in the phylum Firmicutes. The complete sequence revealed that the genome consists of one circular chromosome of 2578146 bps encoding 2451 predicted genes. This is the first genome sequence of an organism belonging to the Haloanaerobiales. Features of both Gram positive and Gram negative bacteria were identified with the presence of both a sporulating mechanism typical of Firmicutes and a characteristic Gram negative lipopolysaccharide being the most prominent. Protein sequence analyses and metabolic reconstruction reveal a unique combination of strategies for thermophilic and halophilic adaptation. H. orenii can serve as a model organism for the study of the evolution of the Gram negative phenotype as well as the adaptation under thermohalophilic conditions and the development of biotechnological applications under conditions that require high temperatures and high salt concentrations. 相似文献
120.
Sieber JR Sims DR Han C Kim E Lykidis A Lapidus AL McDonnald E Rohlin L Culley DE Gunsalus R McInerney MJ 《Environmental microbiology》2010,12(8):2289-2301
Syntrophomonas wolfei is a specialist, evolutionarily adapted for syntrophic growth with methanogens and other hydrogen- and/or formate-using microorganisms. This slow-growing anaerobe has three putative ribosome RNA operons, each of which has 16S rRNA and 23S rRNA genes of different length and multiple 5S rRNA genes. The genome also contains 10 RNA-directed, DNA polymerase genes. Genomic analysis shows that S. wolfei relies solely on the reduction of protons, bicarbonate or unsaturated fatty acids to re-oxidize reduced cofactors. Syntrophomonas wolfei lacks the genes needed for aerobic or anaerobic respiration and has an exceptionally limited ability to create ion gradients. An ATP synthase and a pyrophosphatase were the only systems detected capable of creating an ion gradient. Multiple homologues for β-oxidation genes were present even though S. wolfei uses a limited range of fatty acids from four to eight carbons in length.Syntrophomonas wolfei, other syntrophic metabolizers with completed genomic sequences, and thermophilic anaerobes known to produce high molar ratios of hydrogen from glucose have genes to produce H(2) from NADH by an electron bifurcation mechanism. Comparative genomic analysis also suggests that formate production from NADH may involve electron bifurcation. A membrane-bound, iron-sulfur oxidoreductase found in S. wolfei and Syntrophus aciditrophicus may be uniquely involved in reverse electron transport during syntrophic fatty acid metabolism. The genome sequence of S. wolfei reveals several core reactions that may be characteristic of syntrophic fatty acid metabolism and illustrates how biological systems produce hydrogen from thermodynamically difficult reactions. 相似文献