全文获取类型
收费全文 | 4942篇 |
免费 | 525篇 |
国内免费 | 5篇 |
出版年
2021年 | 86篇 |
2020年 | 62篇 |
2019年 | 60篇 |
2018年 | 78篇 |
2017年 | 82篇 |
2016年 | 107篇 |
2015年 | 160篇 |
2014年 | 184篇 |
2013年 | 221篇 |
2012年 | 235篇 |
2011年 | 225篇 |
2010年 | 163篇 |
2009年 | 140篇 |
2008年 | 219篇 |
2007年 | 201篇 |
2006年 | 171篇 |
2005年 | 170篇 |
2004年 | 168篇 |
2003年 | 157篇 |
2002年 | 173篇 |
2001年 | 137篇 |
2000年 | 117篇 |
1999年 | 105篇 |
1998年 | 64篇 |
1997年 | 53篇 |
1996年 | 61篇 |
1995年 | 64篇 |
1994年 | 52篇 |
1993年 | 47篇 |
1992年 | 73篇 |
1991年 | 90篇 |
1990年 | 79篇 |
1989年 | 73篇 |
1988年 | 67篇 |
1987年 | 67篇 |
1986年 | 58篇 |
1985年 | 59篇 |
1984年 | 52篇 |
1983年 | 50篇 |
1982年 | 39篇 |
1981年 | 48篇 |
1980年 | 43篇 |
1979年 | 45篇 |
1978年 | 48篇 |
1977年 | 41篇 |
1976年 | 42篇 |
1975年 | 55篇 |
1974年 | 65篇 |
1971年 | 52篇 |
1970年 | 39篇 |
排序方式: 共有5472条查询结果,搜索用时 15 毫秒
991.
Melinda J. Griffiths Robert P. van Hille Susan T. L. Harrison 《Applied microbiology and biotechnology》2014,98(5):2345-2356
Chlorella vulgaris accumulates lipid under nitrogen limitation, but at the expense of biomass productivity. Due to this tradeoff, improved lipid productivity may be compromised, despite higher lipid content. To determine the optimal degree of nitrogen limitation for lipid productivity, batch cultures of C. vulgaris were grown at different nitrate concentrations. The growth rate, lipid content, lipid productivity and biochemical and elemental composition of the cultures were monitored for 20 days. A starting nitrate concentration of 170 mg L?1 provided the optimal tradeoff between biomass and lipid production under the experimental conditions. Volumetric lipid yield (in milligram lipid per liter algal culture) was more than double that under nitrogen-replete conditions. Interpolation of the data indicated that the highest volumetric lipid concentration and lipid productivity would occur at nitrate concentrations of 305 and 241 mg L?1, respectively. There was a strong correlation between the nitrogen content of the cells and the pigment, protein and lipid content, as well as biomass and lipid productivity. Knowledge of the relationships between cell nitrogen content, growth, and cell composition assists in the prediction of the nitrogen regime required for optimal productivity in batch or continuous culture. In addition to enhancing lipid productivity, nitrogen limitation improves the lipid profile for biodiesel production and reduces the requirement for nitrogen fertilizers, resulting in cost and energy savings and a reduction in the environmental burden of the process. 相似文献
992.
Leonard G. Forgan Nicholas P. L. Tuckey Denham G. Cook Alistair R. Jerrett 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2014,184(4):437-447
Although spiny rock lobster (Jasus edwardsii) is a wholly sub-littoral species, they show a considerable ability to survive prolonged emersion, a fact exploited during the commercial export of this species. Yet, despite this remarkable hardiness, basic information on how this species responds physiologically to emersion is somewhat lacking. Using flow-through respirometry and electrophysiological techniques, we identified that J. edwardsii undergoes marked physiological changes during rest, emersion and recovery over a broad range of temperatures (3.7–17.8 °C). Under resting conditions, routine metabolic rates (RMR) were 22.57 ± 2.39, 9.69 ± 0.55 and 8.09 ± 0.27 mL O2 h?1, average heart rates (Hr) were 54.72 ± 4.46, 37.68 ± 2.86 and 29.67 ± 0.59 BPM, and ventilation frequencies were 83.71 ± 5.86, 45.34 ± 2.91 and 41.62 ± 0.65 BPM at 15.0, 7.5 and 3.7 °C, respectively. Notably, the surgical implantation of electrodes elevated RMR compared with non-surgical treatments. In surgery and non-surgery groups, Q 10 was calculated to be ca. 3.0. Upon emersion, rate of oxygen consumption and Hr decreased below resting rates in a temperature-dependent manner, but, along with rate of CO2 production, increased steadily during 24-h emersion. Ventilation frequencies upon emersion showed a contrasting response and increased significantly above resting rates. When returned to flow-through sea water for recovery, elevated respiration rates provided clear evidence of an O2 debt, and near-complete recovery was observed after 17 h at both 15.0 and 7.5 °C, but close to no debt was recovered at 3.7 °C. In addition, J. edwardsii was observed to undergo marked diurnal and periodic ventilation cycles, characterised by synchronous changes in RMR, Hr and ventilation frequency. 相似文献
993.
Daniel Burns Lyndon Brooks Peter Harrison Trish Franklin Wally Franklin David Paton Phil Clapham 《Marine Mammal Science》2014,30(2):562-578
Humpback whales (Megaptera novaeangliae) migrate long distances each year on a return journey from low‐latitude breeding grounds to high‐latitude feeding grounds. Migration is influenced by subtle and complex social behaviors and the assumption that whales transit directly through the migratory corridor off the east coast of Australia requires further investigation. From 2003 to 2005, we followed the movements of 99 individual whales within one migratory cycle from three locations, off Byron Bay during the whales' northern migration and in Hervey Bay and at Ballina during the southern migration. The median sighting interval of whales between Byron Bay and Hervey Bay (n = 26) was 52 d (IQR = 42.5–75.5); between Byron Bay and Ballina (n = 21) was 59 d (IQR = 47.0–70.0); and between Hervey Bay and Ballina (n = 33) was 9 d (8.0–14.0). The overall pattern observed from these resightings suggests that Group E1 humpback whales spend approximately two months in the northern quarter of their range during the austral winter months. Intraseason resightings of whales at Ballina (n = 13, median sighting interval = 7 d) also suggest that some individuals, particularly adult males, may circle back north during their general southward journey along this part of the coast, perhaps in an attempt to increase mating opportunities. 相似文献
994.
John C. Whitney Christina M. Beck Young Ah Goo Alistair B. Russell Brittany N. Harding Justin A. De Leon David A. Cunningham Bao Q. Tran David A. Low David R. Goodlett Christopher S. Hayes Joseph D. Mougous 《Molecular microbiology》2014,92(3):529-542
Bacterial secretion systems often employ molecular chaperones to recognize and facilitate export of their substrates. Recent work demonstrated that a secreted component of the type VI secretion system (T6SS), haemolysin co‐regulated protein (Hcp), binds directly to effectors, enhancing their stability in the bacterial cytoplasm. Herein, we describe a quantitative cellular proteomics screen for T6S substrates that exploits this chaperone‐like quality of Hcp. Application of this approach to the Hcp secretion island I‐encoded T6SS (H1‐T6SS) of Pseudomonas aeruginosa led to the identification of a novel effector protein, termed Tse4 (t ype VI s ecretion e xported 4), subsequently shown to act as a potent intra‐specific H1‐T6SS‐delivered antibacterial toxin. Interestingly, our screen failed to identify two predicted H1‐T6SS effectors, Tse5 and Tse6, which differ from Hcp‐stabilized substrates by the presence of toxin‐associated PAAR‐repeat motifs and genetic linkage to members of the valine‐glycine repeat protein G (vgrG) genes. Genetic studies further distinguished these two groups of effectors: Hcp‐stabilized effectors were found to display redundancy in interbacterial competition with respect to the requirement for the two H1‐T6SS‐exported VgrG proteins, whereas Tse5 and Tse6 delivery strictly required a cognate VgrG. Together, we propose that interaction with either VgrG or Hcp defines distinct pathways for T6S effector export. 相似文献
995.
Major changes consistent with the fingerprint of global warming have been reported for nearly every ecosystem on earth. Recently, studies have moved beyond correlation-based inference to demonstrate mechanistic links between warming and biological responses, particularly in regions experiencing rapid change. However, the assessment of climate change impacts and development of adaptation options that humans can undertake are at the earliest stages, particularly for marine systems. Here, we use trends in ocean temperature to characterize regions that can act as natural laboratories or focal points for early learning. These discrete marine ‘hotspots’, where ocean warming is fastest, were identified based on 50 years of historical sea surface temperature data. Persistence of these hotspots into the future was evaluated using global climate models. This analysis provides insights and a starting point for scientists aiming to identify key regions of concern with regard to ocean warming, and illustrates a potential approach for considering additional physical drivers of change such as ocean pH or oxygenation. We found that some hotspot regions were of particular concern due to other non-climate stressors. For instance, many of the marine hotspots occur where human dependence on marine resources is greatest, such as south-east Asia and western Africa, and are therefore of critical consideration in the context of food security. Intensive study and development of comprehensive inter-disciplinary networks based on the hotspot regions identified here will allow earliest testing of management and adaptation pathways, facilitating rapid global learning and implementation of adaptation options to cope with future change. 相似文献
996.
Sergey Ivanov Maria J. Harrison 《The Plant journal : for cell and molecular biology》2014,80(6):1151-1163
Medicago truncatula is widely used for analyses of arbuscular mycorrhizal (AM) symbiosis and nodulation. To complement the genetic and genomic resources that exist for this species, we generated fluorescent protein fusions that label the nucleus, endoplasmic reticulum, Golgi apparatus, trans‐Golgi network, plasma membrane, apoplast, late endosome/multivesicular bodies (MVB), transitory late endosome/ tonoplast, tonoplast, plastids, mitochondria, peroxisomes, autophagosomes, plasmodesmata, actin, microtubules, periarbuscular membrane (PAM) and periarbuscular apoplastic space (PAS) and expressed them from the constitutive AtUBQ10 promoter and the AM symbiosis‐specific MtBCP1 promoter. All marker constructs showed the expected expression patterns and sub‐cellular locations in M. truncatula root cells. As a demonstration of their utility, we used several markers to investigate AM symbiosis where root cells undergo major cellular alterations to accommodate their fungal endosymbiont. We demonstrate that changes in the position and size of the nuclei occur prior to hyphal entry into the cortical cells and do not require DELLA signaling. Changes in the cytoskeleton, tonoplast and plastids also occur in the colonized cells and in contrast to previous studies, we show that stromulated plastids are abundant in cells with developing and mature arbuscules, while lens‐shaped plastids occur in cells with degenerating arbuscules. Arbuscule development and secretion of the PAM creates a periarbuscular apoplastic compartment which has been assumed to be continuous with apoplast of the cell. However, fluorescent markers secreted to the periarbuscular apoplast challenge this assumption. This marker resource will facilitate cell biology studies of AM symbiosis, as well as other aspects of legume biology. 相似文献
997.
Mark J. Statham James Murdoch Jan Janecka Keith B. Aubry Ceiridwen J. Edwards Carl D. Soulsbury Oliver Berry Zhenghuan Wang David Harrison Malcolm Pearch Louise Tomsett Judith Chupasko Benjamin N. Sacks 《Molecular ecology》2014,23(19):4813-4830
Widely distributed taxa provide an opportunity to compare biogeographic responses to climatic fluctuations on multiple continents and to investigate speciation. We conducted the most geographically and genomically comprehensive study to date of the red fox (Vulpes vulpes), the world's most widely distributed wild terrestrial carnivore. Analyses of 697 bp of mitochondrial sequence in ~1000 individuals suggested an ancient Middle Eastern origin for all extant red foxes and a 400 kya (SD = 139 kya) origin of the primary North American (Nearctic) clade. Demographic analyses indicated a major expansion in Eurasia during the last glaciation (~50 kya), coinciding with a previously described secondary transfer of a single matriline (Holarctic) to North America. In contrast, North American matrilines (including the transferred portion of Holarctic clade) exhibited no signatures of expansion until the end of the Pleistocene (~12 kya). Analyses of 11 autosomal loci from a subset of foxes supported the colonization time frame suggested by mtDNA (and the fossil record) but, in contrast, reflected no detectable secondary transfer, resulting in the most fundamental genomic division of red foxes at the Bering Strait. Endemic continental Y‐chromosome clades further supported this pattern. Thus, intercontinental genomic exchange was overall very limited, consistent with long‐term reproductive isolation since the initial colonization of North America. Based on continental divergence times in other carnivoran species pairs, our findings support a model of peripatric speciation and are consistent with the previous classification of the North American red fox as a distinct species, V. fulva. 相似文献
998.
999.
1000.