全文获取类型
收费全文 | 368篇 |
免费 | 27篇 |
出版年
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 14篇 |
2020年 | 5篇 |
2019年 | 9篇 |
2018年 | 13篇 |
2017年 | 12篇 |
2016年 | 18篇 |
2015年 | 20篇 |
2014年 | 27篇 |
2013年 | 24篇 |
2012年 | 41篇 |
2011年 | 23篇 |
2010年 | 16篇 |
2009年 | 13篇 |
2008年 | 28篇 |
2007年 | 21篇 |
2006年 | 31篇 |
2005年 | 13篇 |
2004年 | 4篇 |
2003年 | 14篇 |
2002年 | 16篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有395条查询结果,搜索用时 15 毫秒
391.
Worrawat Promden Alisa S. Vangnai Piamsook Pongsawasdi Osao Adachi Kazunobu Matsushita & Hirohide Toyama 《FEMS microbiology letters》2008,280(2):203-209
Pseudomonas putida HK5 produces three different quinoprotein alcohol dehydrogenases: ADH-I, ADH-IIB and ADH-IIG. Gene organization of qedA , the gene for ADH-I, and other 10 genes in the cluster was related to the genome sequences of five other Pseudomonas strains. Insertion mutations in either qedA , exaE or agmR eliminated ADH-I activity, although the mutants were still able to grow on ethanol but more slowly than the wild-type strain. Mutant analysis demonstrated the requirement of agmR and exaE in ADH-I expression, and the tentative involvement of agmR , but not exaE , in the induction of ADH-IIB and ADH-IIG activities. 相似文献
392.
This paper documents the Penicillium species associated with the naturally occurring plants and animals in the Windmill Islands, East Antarctica. An attempt has
been made to establish the species associations between the Penicillia and the mosses and birds. The species found are discussed
briefly in terms of taxonomy and biological significance, and a new species, Penicillium antarcticum, is described.
Accepted: 27 July 1998 相似文献
393.
394.
Izabela Sokolowska Armand G. Ngounou WetieUrmi Roy Alisa G. WoodsCostel C. Darie 《Biochimica et Biophysica Acta - Proteins and Proteomics》2013,1834(8):1474-1483
We used a targeted proteomics approach to investigate whether introduction of new N-linked glycosylation sites in a chimeric protein influence the glycosylation of the existing glycosylation sites. To accomplish our goals, we over-expressed and purified a chimeric construct that contained the Fc region of the IgG fused to the exons 7 & 8 of mouse ZP3 (IgG-Fc-ZP3E7 protein). Immunoglobulin heavy chain (IgG-HC protein) was used as control. We then analyzed the IgG-HC and IgG-Fc-ZP3E7 proteins by liquid chromatography-tandem mass spectrometry (LC–MS/MS) and by Western blotting (WB). We concluded that in control experiments, the glycosylation site was occupied as expected. However, in the IgG-Fc-ZP3E7 protein, we concluded that only one out of three NXS/T glycosylation sites is occupied by N-linked oligosaccharides. We also concluded that in the IgG-Fc-ZP3E7 protein, upon introduction of additional potential NXS/T glycosylation sites within its sequence, the original NST/S glycosylation site from the Fc region of the IgG-Fc-ZP3E7 protein is no longer glycosylated. The biomedical significance of our findings is discussed. 相似文献
395.
Mikhaylina Alisa O. Nikonova Ekaterina Y. Kostareva Olga S. Piendl Wolfgang Erlacher Matthias Tishchenko Svetlana V. 《Biochemistry. Biokhimii?a》2021,86(4):397-408
Biochemistry (Moscow) - Ribosomal protein L1 is a conserved two-domain protein that is involved in formation of the L1 stalk of the large ribosomal subunit. When there are no free binding sites... 相似文献