排序方式: 共有24条查询结果,搜索用时 500 毫秒
11.
Sujatha Venkataraman Diane K. Birks Ilango Balakrishnan Irina Alimova Peter S. Harris Purvi R. Patel Michael H. Handler Adrian Dubuc Michael D. Taylor Nicholas K. Foreman Rajeev Vibhakar 《The Journal of biological chemistry》2013,288(3):1918-1928
Aberrant expression of microRNAs has been implicated in many cancers. We recently demonstrated differential expression of several microRNAs in medulloblastoma. In this study, the regulation and function of microRNA 218 (miR-218), which is significantly underexpressed in medulloblastoma, was evaluated. Re-expression of miR-218 resulted in a significant decrease in medulloblastoma cell growth, cell colony formation, cell migration, invasion, and tumor sphere size. We used C17.2 neural stem cells as a model to show that increased miR-218 expression results in increased cell differentiation and also decreased malignant transformation when transfected with the oncogene REST. These results suggest that miR-218 acts as a tumor suppressor in medulloblastoma. MicroRNAs function by down-regulating translation of target mRNAs. Targets are determined by imperfect base pairing of the microRNA to the 3′-UTR of the mRNA. To comprehensively identify actual miR-218 targets, medulloblastoma cells overexpressing miR-218 and control cells were subjected to high throughput sequencing of RNA isolated by cross-linking immunoprecipitation, a technique that identifies the mRNAs bound to the RNA-induced silencing complex component protein Argonaute 2. High throughput sequencing of mRNAs identified 618 genes as targets of miR-218 and included both previously validated targets and many targets not predicted computationally. Additional work further confirmed CDK6, RICTOR, and CTSB (cathepsin B) as targets of miR-218 and examined the functional role of one of these targets, CDK6, in medulloblastoma. 相似文献
12.
K. D. Davranov K. A. Gulyamova B. Kh. Alimova N. M. Turapova 《Applied Biochemistry and Microbiology》2000,36(1):19-22
Enzymatic hydrolysis of neutral fat of cotton oil soap stock with a nonspecific lipase produced byOospora lactis F-500 was designed. The culture liquid and a preparation of enzyme obtained by precipitation with isopropanol from a filtrate
of the culture liquid were used. Utilization of cotton oil soap stock as the only source of carbon during cultivation of the
fungus was studied. The rate of hydrolysis of soap stock fat strongly depended on the way of biological conversion of cotton
oil soap stock. The most effective utilization was observed during cultivation of the fungus in the medium containing soap
stock. 相似文献
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A T Astvatsatur'yan Y M Ostrovskii E K Alimova F S Larin Z I Shilova I M Staviskii V U Buko 《Biology bulletin of the Academy of Sciences of the USSR》1979,6(5):643-645
Incubation of tissue of homogenates of peripheral nerves, liver, and subcutaneous adipose tissue of rabbits with [1-14C]palmitic acid and intraperitoneal injection of this preparation into rats, in a dose of 20 muCi per 100 g of weight of the animals, permitted establishment of the presence of enzyme systems of biosynthesis of n-alkanes in the tissues of higher animals. Evidence of the biosynthesis of n-alkanes in the animal organism represents a new aspect of our knowledge of the metabolism of this class of compounds in animal tissues. 相似文献
15.
A. A. Abd El-Rahman O. V. Kozlova S. M. A. El-Shafei F. K. Alimova F. G. Kupriyanova-Ashina 《Microbiology》2014,83(4):344-351
Growth patterns and intracellular Ca2+ concentrations in the mutant strain Aspergillus awamori 66A containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin B. The cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity of sporulation) was dose-dependent. Low concentrations of amphotericin B (2.5 μM) stimulated spore germination: the number of germinating spores was 2–3 times higher than in the control (without the fungicide). At higher amphotericin concentrations (20 μM) spore germination was inhibited. Amphotericin B had a dose-dependent effect on mycelial growth and sporulation intensity on solid Vogel medium. Intracellular Ca2+ concentrations in the presence of amphotericin B were investigated using the luminescence of the photoprotein aequorin. High concentrations of amphotericin B (10 and 20 μM) were shown to cause an instantaneous increase in Ca2+ concentrations compared to the control and lower amphotericin concentration (2.5 μM). Ca2+ concentrations remained elevated throughout the experiment and correlated with the inhibition of mycelial growth and development. 相似文献
16.
Sujatha Venkataraman Irina Alimova Rong Fan Peter Harris Nicholas Foreman Rajeev Vibhakar 《PloS one》2010,5(6)
Background
MicroRNAs (miRNAs) are a class of short non-coding RNAs that regulate cell homeostasis by inhibiting translation or degrading mRNA of target genes, and thereby can act as tumor suppressor genes or oncogenes. The role of microRNAs in medulloblastoma has only recently been addressed. We hypothesized that microRNAs differentially expressed during normal CNS development might be abnormally regulated in medulloblastoma and are functionally important for medulloblastoma cell growth.Methodology and Principal Findings
We examined the expression of microRNAs in medulloblastoma and then investigated the functional role of one specific one, miR-128a, in regulating medulloblastoma cell growth. We found that many microRNAs associated with normal neuronal differentiation are significantly down regulated in medulloblastoma. One of these, miR-128a, inhibits growth of medulloblastoma cells by targeting the Bmi-1 oncogene. In addition, miR-128a alters the intracellular redox state of the tumor cells and promotes cellular senescence.Conclusions and Significance
Here we report the novel regulation of reactive oxygen species (ROS) by microRNA 128a via the specific inhibition of the Bmi-1 oncogene. We demonstrate that miR-128a has growth suppressive activity in medulloblastoma and that this activity is partially mediated by targeting Bmi-1. This data has implications for the modulation of redox states in cancer stem cells, which are thought to be resistant to therapy due to their low ROS states. 相似文献17.
Kh Ia Karimov A S Komarin T E Lysenko L N Davletshina A F Alimova 《Biulleten' eksperimental'no? biologii i meditsiny》1992,114(8):145-148
Histamine impact on lipid peroxidation (LPO) in hepatic and pulmonary microsomes of guinea-pigs sensibilization was studied. It is demonstrated that intensity of chemiluminescence and LPO product content in NADPH-, ascorbat-dependent and spontaneous systems was enhanced in relation to the period of sensibilization process formation and histamine concentration. 相似文献
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Davranov KD Guliamova KA Alimova BKh Turapova NM 《Prikladnaia biokhimiia i mikrobiologiia》2000,36(1):26-29
Enzymatic hydrolysis of neutral fat of cotton oil soap stock with a nonspecific lipase produced by Oospora lactis F-500 was designed. The culture liquid and a preparation of enzyme obtained by precipitation with isopropanol from a filtrate of the culture liquid were used. Utilization of cotton oil soap stock as the only source of carbon during cultivation of the fungus was studied. The rate of hydrolysis of soap stock fat strongly depended on the way of biological conversion of cotton oil soap stock. The most effective utilization was observed during cultivation of the fungus in the medium containing soap stock. 相似文献
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