High velocity mechanical injection of foreign DNA into fish oocytes

High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.     

Genomic fingerprinting: new possibilities in determining the species identity of Brucella

The genomes of various Brucella species were shown to contain hypervariable sequences which can be detected by the M13 DNA. It has been demonstrated that the method of DNA fingerprinting opens up new possibilities in differentiation and identification of Brucellosis agents.