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11.
Bovine-heart mitochondrial complex III was purified in the presence of Triton X-100, and the size and shape of the resulting protein-surfactant-phospholipid mixed micelles were investigated by laser light-scattering. The protein appears to be present in the form of a dimer, irrespective of temperature (between 25 and 40 degrees C) and protein concentration (between 0.5 and 5 mg/ml). The molecular weight of the micelle increases with temperature from 600 000 (25 degrees C) to 692 000 (40 degrees C). The variation of the solvent second virial coefficient in this temperature range suggests that, with increasing temperature, some of the free surfactant molecules become integrated in the mixed micelles. The average quadratic radius of gyration of these is of 42 +/- 5 nm, corresponding in our case to an ellipsoidal shape. 相似文献
12.
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14.
M. R. Sarić 《Plant and Soil》1987,99(1):197-209
Summary The paper discusses the problems of genetic aspects of plant mineral nutrition in the light of the results presented at the
First and Second Symposia on ‘Genetic Aspects of Plant Mineral Nutrition’ organized in Beograd in 1982 and Madison in 1985,
respectively. On the basis of the results, future directions of research are discussed. The papers deal with the concentration
and content of mineral nutrients in different genotypes, physiological and biochemical aspects of the genetic specificity
of plant mineral nutrition, relations between plant genotypes and nitrogen fixing micro-organism strains, as well as with
some related problems which have been investigated to a lesser extent. Particular attention is paid to papers and problems
referring to genetic and breeding research work linked with genetic aspects of plant mineral nutrition as well as the possibilities
of developing new cultivars requiring certain soil and mineral nutrition conditions for their cultivation. 相似文献
15.
The Xenopus laevis genome was screened for putative DNA-binding gene products by using the 'finger' region of the Drosophila gene Krüppel as a probe. The one gene detected, named Xfin, codes for a protein with 37 finger domains that comprise nearly 90% of the protein. In the light of studies by Rhodes and Klug (Cell, 46, 123-132, 1986), these data suggest that the Xfin protein has the capacity to bind an unusually large stretch (185 bases) of DNA. The Xfin gene is expressed as a maternal and zygotic mRNA that undergoes extensive polyadenylation changes during early development. The Xfin mRNA expression pattern and the potential DNA binding activity of the protein point to the possibility that the Xfin gene may have a role in controlling gene activity during early embryonic development. 相似文献
16.
Elžbieta Milewska-Pawliczuk 《Biologia Plantarum》1987,29(4):295-298
Six thousand anthers ofSecale cereale cv. Dańkowskie Zlote and inbred lines L214 L215 and L258c/5, in the uninuclear microspore were cultured over a period of
three months on Murashige and Skoog nutrient medium supplemented with IAA (1–2μg1−1), kinetin (0.2 to (1.0μg1−1) and 2,4-D (0.5–2.0μg1−1). First divisions of microspore were observed after 7 days. In the course of 10 weeks, 4 albino tic embryos at the cotyledon
stage were observed that died away in the course of further culture. The course of androgenesis was regular in inbred lines
and irregular in rye cv. Dańkowskie Ziote. The efficiency of androgenesis and the amount of observed globular structures in
anthers were also dependent on the genetic potency of the material. Inbred lines did not show any greater viability of embryos. 相似文献
17.
Mapping of the gene for anti-müllerian hormone to the short arm of human chromosome 19 总被引:3,自引:0,他引:3
O Cohen-Haguenauer J Y Picard M G Mattéi S Serero V C Nguyen M F de Tand D Guerrier M C Hors-Cayla N Josso J Frézal 《Cytogenetics and cell genetics》1987,44(1):2-6
The gene coding for human anti-Müllerian hormone (AMH) was localized to subbands p13.2----p13.3 on chromosome 19, using in situ hybridization and Southern blot analysis of a panel of man-mouse and man-hamster somatic cell hybrids. 相似文献
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Aleš Vančura Ivana Vančurová Jan Kopecký Jaroslav Maršálek Daniel Cikánek Gabriela Basařová Vladimir Křišťan 《Archives of microbiology》1989,151(6):537-540
Synthesis of threonine dehydratase in Streptomyces fradiae was positively influenced by valine and negatively by isoleucine. However, these two amino acids had no effect on the activity of this enzyme. Synthesis of threonine dehydratase in -aminobutyrate resistant mutants of S. fradiae was pronouncedly less sensitive to the positive effect of valine and this change in regulation led to valine overproduction. Synthesis of acetohydroxy acid synthase is regulated in a similar manner to that of threonine dehydratase, however a lower level of expression was detected in -aminobutyrate resistant mutants. And again, no effect of branched-chain amino acids on acetohydroxy acid synthase activity was observed. It follows that in S. fradiae synthesis of threonine dehydratase is the main regulatory mechanism governing production and the mutual ratio of synthesized valine and isoleucine.Abbreviations -AB
-aminobutyrate
- AHAS
acetohydroxy acid synthase
- -KB
-ketobutyrate
- MNNG
N-methyl-N-nitro-N-nitrosoguanidine
- TD
threonine dehydratase
- Trans. B.
transaminase of branched-chain amino acids
- VDH
valine dehydrogenase 相似文献
20.
In the lichen genusUsnea different species ofTrebouxia-phycobionts as well as different haustorial types are known. The isolated and cultivated phycobiont ofUsnea longissima
Ach. was studied by light- and electron microscopy and resembles in cytomorphological details the type ofTrebouxia impressa
Ahmad. In addition to simple wall-to-wall contacts between the symbiotic components also intraparietal (=intrawall-)haustoria could be observed as the normal interaction type.
Frau Prof. Dr.Elisabeth Tschermak-Woess zu ihrem 70. Geburtstag gewidmet. 相似文献