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61.
62.
To assess the effect of conformational mobility on receptor activity, the β-phenyl substituent of dopamine D(1) agonist ligands of the phenylbenzazepine class, (±)-6,6a,7,8,9,13b-hexahydro-5H-benzo[d]naphtho[2,1-b]azepine-11,12-diol (8), and its oxygen and sulfur bioisosteres 9 and 10, respectively, were synthesized as conformationally-restricted analogs of SKF38393, a dopamine D(1)-selective partial agonist. Compounds trans-8b, 9, and 10 showed binding affinity comparable to that of SKF38393, but functionally, they displayed only very weak agonist activity. These results suggest that the conformationally-restricted structure of the analogs cannot adopt a binding orientation that is necessary for agonist activity. 相似文献
63.
Najla Trabelsi Hanen Falleh Ines Jallali Alia Ben Daly Hafedh Hajlaoui Abderrazek Smaoui Chedly Abdelly Riadh Ksouri 《Acta Physiologiae Plantarum》2012,34(1):87-96
Changes in phenolic composition and biological activities were investigated in different Limoniastrum monopetalum L. organs. For that, 80% aqueous acetone extracts were used to estimate total phenolic contents and their antioxidant activities
were evaluated using DPPH· and O2·− radical scavenging activities and reducing power. The efficiency of organ extracts was tested against human pathogen strains.
Ultimately, acid hydrolysis of all organs was subjected to RP-HPLC for phenolic identification. Results showed that flower
extracts exhibited the highest polyphenol (65.42 GAE/g DW) and flavonoid (35.36 CE/g DW) contents. Stems were enriched in
condensed tannin content (21.4 mg CE/g DW) and displayed the best antiradical activities and the highest reducing power. Besides,
stem and gall extracts showed the highest efficiency against pathogenic bacteria as compared with those of flower. Concerning
the antifungal test, a slight activity was found in gall extracts. The RP-HPLC showed a difference in phenolic compounds that
varied as function of organ. In fact, the major phenolic compound varied as function of organ. Results suggest that L. monopetalum could be a promising source of biomolecules for therapeutic and nutraceutical industries and the difference between organs
may be related to their physiological role. 相似文献
64.
Enhancement of the tolerance of
Arabidopsis
to high temperatures by genetic engineering of the synthesis of glycinebetaine 总被引:6,自引:1,他引:5
Alia Hidenori Hayashi Atsushi Sakamoto Norio Murata 《The Plant journal : for cell and molecular biology》1998,16(2):155-161
Arabidopsis thaliana was transformed with the codA gene for choline oxidase from Arthrobacter globiformis under control of the 35S RNA promoter of cauliflower mosaic virus. As a result, high levels of glycinebetaine accumulated in the seeds of transformed plants. Transformation with the codA gene significantly enhanced the tolerance to high temperatures during the imbibition and germination of seeds, as well as during growth of young seedlings. The extent of enhancement of the tolerance to high temperature was correlated with levels of choline oxidase expressed and of glycinebetine accumulated in the transformed plants. The induction of homologues of heat shock protein 70 at high temperature was less conspicuous in the transformed plants than in the wild-type plants, suggesting that the transformation alleviated the high-temperature stress. 相似文献
65.
Immunohistochemical observations on tubulin and alpha actinin during mitosis of cultured fibroblasts
N Arena G Vergani R Madeddu G Satta L Deiana E E Alia 《Bollettino della Società italiana di biologia sperimentale》1982,58(23):1552-1557
In this work we studied alpha-actinin and tubulin sites in rabbit fibroblasts in culture. Antibodies anti-alpha-actinin were used for indirect PAP-reaction while antibodies anti-tubulin were used for indirect immunofluorescence method. The observations were carried out by light microscopy, phase-contrast and interference-contrast microscopy, with regard to actinin, and by fluorescence microscopy, with regard to tubulin. During the early mitotic phase, alpha-actinin is localized all over the cell membrane of the fibroblasts, forming a sort strong protective cap, while during diacinesis it forms only rings, localizing below the cell membrane and the philopodia. Thus tubulin forms the bundle fibres during mitotic phases. 相似文献
66.
Zarazvand Farjad Karimi Maryam Moosavian Seyedeh Alia Arabi Leila Badiee Ali Jaafari Mahmoud Reza Mashreghi Mohammad 《International journal of peptide research and therapeutics》2021,27(3):2099-2109
International Journal of Peptide Research and Therapeutics - This study aimed to evaluate the antitumor activity of PEGylated liposomal doxorubicin (Dox) functionalized with TAT peptide through... 相似文献
67.
68.
Extravillous trophoblast (EVT) cells of the human placenta invade the uterine decidua and utero-placental arteries to establish an efficient exchange of key molecules between maternal and fetal blood. Trophoblast invasion is stringently regulated in situ both positively and negatively by a variety of factors at the fetal-maternal interface to maintain a healthy utero-placental homeostasis. One such factor, decorin, a transforming growth factor (TGF)-beta binding, leucine-rich proteoglycan produced by the decidua, negatively regulates EVT proliferation, migration, and invasiveness independent of TGF-beta. We reported that these decorin actions were mediated by its binding to multiple tyrosine kinase receptors, including vascular endothelial growth factor receptor (VEGFR)-2. The present study explores the mechanisms underlying decorin antagonism of VEGF (VEGF-A) stimulation of endovascular differentiation of EVT using our EVT cell line, HTR-8/SVneo. We observe that decorin inhibits VEGF-induced EVT cell migration and endothelial-like tube formation on matrigel. VEGF activates MAPKs (p38 MAPK, MEK3/6, and ERK1/2) in EVT cells, and the activation is blocked in both cases by decorin. Employing selective MAPK inhibitors, we show that both p38 and ERK pathways contribute independently to VEGF-induced EVT migration and capillary-like tube formation. VEGF upregulates the vascular endothelial (VE) markers VE-cadherin and beta-catenin in EVT and endothelial cells, and this upregulation is blocked by decorin and MAPK inhibitors. These results suggest that decorin inhibits VEGF-A stimulation of trophoblast migration and endovascular differentiation by interfering with p38 MAPK and ERK1/2 activation. Thus decorin-mediated dual impediment of endovascular differentiation of the EVT and angiogenesis may have implications for pathogenesis of preeclampsia, a hypoinvasive trophoblast disorder in pregnancy. 相似文献
69.
Effects of lectins on cytoskeleton and morphology or cultured chick embryo fibroblasts 总被引:1,自引:0,他引:1
N Arena M Bodo T Baroni F A Alia L Gaspa E Becchetti 《Cellular and molecular biology, including cyto-enzymology》1990,36(3):317-328
The microfilaments and microtubules of cultured chick embryo skin fibroblasts were studied in the presence of exogenous lectins by an indirect immunofluorescence technique. Lectin treatment induced modifications in the arrangement of myosin, actin and tubulin, determined depolymerization of the proteins and caused changes in cell shape and size. The results suggest that the interaction between lectins and their specific membrane receptors triggers a series of changes in the cytoskeletal pattern via transmembrana as yet unknown mechanisms and that these are responsible for the alterations in cell shape and size. 相似文献
70.
Differential selection pressures caused by environmental disparities lead to populations to become differentiated as they adapt to local environments. In addition, natural selection during the species past can contribute to the observed differentiation. In this study, we examine the geographic variation in a set of four traits related to growth and plant architecture in cork oak (Quercus suber) and investigate to what extent this variation is the result of the effects of ongoing evolution in current environments and the past evolutionary history of the species. Cork oak saplings at the common garden trial exhibited differences in plant architecture associated to cpDNA lineage. Eastern lineages, exhibited the lowest apical dominance and highest branchiness, consistent with the analyses in other cork oak trials. In contrast, patterns linked to the evolutionary past were less evident in height and diameter. These results suggest that selective pressures after cpDNA divergence can have blurred patterns in some traits closely related to fitness, while conserving the past evolutionary imprints in plant architectural traits. Introgressed populations did not show significant differentiation in architecture, which suggests that allele exchanges via hybridization have had a limited effect on population differentiation in cork oak. Finally, populations within lineages also showed differences in growth and architecture. Correlation between population architecture and temperature patterns were observed indicating that environmental factors such as climate also could result crucial in the evolution of plant architecture of cork oak within lineages. 相似文献