Production of Specific Agglutinins for Mycobacteria in Peritoneal Fluid of Mice

Specific agglutinins were produced in ascitic fluid of mice by intraperitoneal injection of certain mycobacteria suspended in Freund's incomplete adjuvant.     

Der Einfluß von Metachromasie und Photodekomposition auf die quantitative Feulgen-DNS Fluoreszenzcytophotometrie

Zusammenfassung Zur quantitativen Bestimmung der Feulgen-DNS-Mengen wurden Leberzellkerne mit Acriflavin fluorochromiert. Durch fluoreszenzmikrospektrographische Untersuchung konnte gezeigt werden, daß die Meßergebnisse nicht durch Metachromasie beeinträchtigt werden. Bei der Photodekomposition kommt es zu keiner Verschiebung der relativen spektralen Intensitätsverteilung. Die Histogramme der Feulgen-DNS-Mengen von di- und tetraploiden Leberzellen zeigen die exakte Einhaltung der Ploidiestufen und eine geringe Streuung der Meßwerte.

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Influence of metachromasia and photofading on the quantitative Feulgen-Dna fluorescence cytophotometry

Summary For the purpose of quantitative determination of Feulgen-DNA liver nuclei had been stained with fluorescent Schiff-type dye Acriflavin according to Böhm and Sprenger (1968). Fluorescence microspectrophotometric investigations revealed that the quantitative measuring results are not falsified by metachromasia and that photofading does not cause a shift in the relative spectral distribution of fluorescence intensity.The Feulgen-DNA histograms of di- and tetraploid liver nuclei confirm the accuracy of quantitative measurements by exact reproduction of ploidy classes and low variation range in each class.

     

Fluorescence cytophotometry: a valuable method for the quantitative determination of nuclear feulgen-DNA

Summary A cytofluorometric apparatus with incident illumination for fluorescence excitation is described here.Cytophotometric fluorescence measurements (UV- and blue excitation) of acriflavine-acridine yellow-, coriphosphine- and pararosaniline-Schiff stained di-, tetra- and octoploid liver nuclei, leucocytes and sperms (Feulgen reaction) were found to agree with the absorbance data obtained from the same slide by means of the integrating microdensitometer.The stoichiometry of the fluorescence emission is discussed in detail. It is emphasized that not a linear, but an exponential relationship exists between the emitted fluorescence intensity and the concentration of the fluorescent substance.Cytofluorometry of Feulgen-stained nuclei has proved to be as reliable and fast as the absorbance scanning measurements at the intergrating microdensitometer.     

Katabolismus von 4′,6-dihydroxyauron in pflanzlichen zellsuspensionskulturen

Cell suspension cultures of Glycine max, Phaseolus aureus, Cicer arietinum and Petroselinum hortense were shown to catabolize (α - ¹⁴C) - 4′,6-dihydroxyaurone as measured by ¹⁴CO₂ production and isolation of (¹⁴C)-p-hydroxybenzoic acid. Aurone catabolism in plants is thus comparable with the degradation of chalcones flavanones and flavonols because in all cases the B-ring is liberated as a substituted benzoic acid.     

Physicochemical modifications of lysosomal hydrolases during intracellular transport

1. The following fractions were prepared from rat kidney and characterized ultrastructurally, biochemically and enzymically: (a) an ordinary rough microsomal (RM(1)) fraction; (b) a special rough microsomal (RM(2)) fraction enriched seven- to nine-fold in acid hydrolases over the homogenate; (c) a smooth microsomal (SM) fraction; (d) a Golgi (GM) fraction enriched 2.5-fold in acid hydrolases and 10-, 15- and 20-fold in sialyltransferase, N-acetyl-lactosamine synthetase and galactosyltransferase respectively; (e) a lysosomal (L) fraction enriched 15- to 23-fold in acid hydrolases. The frequency of Golgi sacs and tubules seen in the electron microscope and the specific activity of the three glycosyltransferases in these fractions increased in the order: RM(2)相似文献   

Das Vorkommen von Bacteriochlorophyll aP und aGg in Stämmen aller Arten der Rhodospirillaceae

Summary The bacteriochlorophylls a of 60 strains belonging to 13 different species of the purple nonsulfur bacteria (Rhodospirillaceae) were studied with respect to the nature of the esterifying alcohol. The new bacteriochlorophyll a_Gg containing all-trans-geranylgeraniol is the main bacteriochlorophyll in all strains of Rhodospirillum rubrum. Rhodospirillum photometricum contains the new and the classical bacteriochlorophyll a_P (phytol as esterifying alcohol) in nearly equal amounts. The strains of all other species contain the classical bacteriochlorophyll a_P.