全文获取类型
收费全文 | 298篇 |
免费 | 25篇 |
专业分类
323篇 |
出版年
2021年 | 5篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 10篇 |
2014年 | 12篇 |
2013年 | 16篇 |
2012年 | 18篇 |
2011年 | 13篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 15篇 |
2006年 | 10篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 8篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 7篇 |
1988年 | 10篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1982年 | 3篇 |
1979年 | 6篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1970年 | 6篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1967年 | 5篇 |
1966年 | 5篇 |
1965年 | 3篇 |
排序方式: 共有323条查询结果,搜索用时 15 毫秒
71.
J. Brian Alford 《Hydrobiologia》2014,738(1):129-146
This study determined if a spatial hierarchy existed with respect to reach-scale habitat, subwatershed-scale, and watershed-scale geomorphology and land use stressors on fish assemblages in southeastern U.S. coastal plain streams. During May–October 2009–2012, fishes were sampled by seine, and habitat was assessed at 50 reaches in the Lake Pontchartrain Basin (USA). Using partial redundancy analysis (pRDA) a variance decomposition procedure was used to partial out influences of confounding covariables at each spatial scale. Reach-scale habitat had the strongest association with the assemblage. Stream width, depth, aquatic vegetation and human debris cover, rapid habitat assessment score, and large woody debris volume were the most important variables. At subwatershed and watershed scales, natural and anthropogenic characteristics were important, including elevation, gradient, watershed area, wetland cover, stream density, road, dam and oil/gas well densities. Six species were associated most strongly with the watershed variables, compared to reach- and subwatershed-scale variables. These species had more “r-selected” life-history strategies (e.g., smaller eggs, shorter life spans, multiple broods, longer spawning season, and trophic generalists). In contrast, most species that were associated strongest with reach-scale variables exhibited more “k-selected” life-history traits (e.g., larger eggs, longer life spans, shorter spawning season, single brood, and trophic specialists). 相似文献
72.
73.
The protein synthetic activities of membrane-bound and free hepatic ribosomes isolated from intact rats fed ad libitum, and normal rats subjected to food restriction to match that of hypophysectomised (Hx) rats were compared to the in vitro protein synthetic capacity of hepatic ribosomes isolated from Hx rats. Hypophysectomy resulted in decreased protein synthetic ability of bound ribosomes, whether protein synthesis was directed by endogenous messenger RNA (mRNA) (p less than 0.05) or by polyuridylic acid (polyU) (p less than 0.01). In contrast, the protein synthetic activity of free hepatic ribosomes from Hx rats was reduced when protein synthesis was directed by endogenous mRNA (p less than 0.05) but, when polyU was substituted as the messenger, the protein synthetic activity of these free ribosomes was equal to that of control rats. On the other hand the effects of food restriction on hepatic ribosomal function could be clearly differentiated from the effects observed following hypophysectomy. Thus, the reduced protein synthetic activity of hepatic bound ribosomes isolated from food restricted normal rats was not demonstrable, when polyU was used to direct protein synthesis. Further, food restriction had no effect on the protein synthetic activity of free hepatic ribosomes, and this was true when protein synthesis was directed by either endogenous or artificial messenger. It is concluded that hypophysectomy reduces the protein synthetic ability of both bound and free hepatic ribosomes, and this change of ribosomal function of Hx rats cannot be attributed to their decreased food intake. 相似文献
74.
Christopher M Rantzau C Chen ZP Snow R Kemp B Alford FP 《American journal of physiology. Endocrinology and metabolism》2006,291(5):E1131-E1140
AMPK plays a central role in influencing fuel usage and selection. The aim of this study was to analyze the impact of low-dose AMP analog 5-aminoimidazole-4-carboxamide-1-beta-d-ribosyl monophosphate (ZMP) on whole body glucose turnover and skeletal muscle (SkM) glucose metabolism. Dogs were restudied after prior 48-h fatty acid oxidation (FA(OX)) blockade by methylpalmoxirate (MP; 5 x 12 hourly 10 mg/kg doses). During the basal equilibrium period (0-150 min), fasting dogs (n = 8) were infused with [3-(3)H]glucose followed by either 2-h saline or AICAR (1.5-2.0 mg x kg(-1) x min(-1)) infusions. SkM was biopsied at completion of each study. On a separate day, the same protocol was undertaken after 48-h in vivo FA(OX) blockade. The AICAR and AICAR + MP studies were repeated in three chronic alloxan-diabetic dogs. AICAR produced a transient fall in plasma glucose and increase in insulin and a small decline in free fatty acid (FFA). Parallel increases in hepatic glucose production (HGP), glucose disappearance (R(d tissue)), and glycolytic flux (GF) occurred, whereas metabolic clearance rate of glucose (MCR(g)) did not change significantly. Intracellular SkM glucose, glucose 6-phosphate, and glycogen were unchanged. Acetyl-CoA carboxylase (ACC approximately pSer(221)) increased by 50%. In the AICAR + MP studies, the metabolic responses were modified: the glucose was lower over 120 min, only minor changes occurred with insulin and FFA, and HGP and R(d tissue) responses were markedly attenuated, but MCR(g) and GF increased significantly. SkM substrates were unchanged, but ACC approximately pSer(221) rose by 80%. Thus low-dose AICAR leads to increases in HGP and SkM glucose uptake, which are modified by prior FA(ox) blockade. 相似文献
75.
Invasive species are widely viewed as unmitigated ecological catastrophes, but the reality is more complex. Theoretically, invasive species could have negligible or even positive effects if they sufficiently reduce the intensity of processes regulating native populations. Understanding such mechanisms is crucial to predicting ultimate ecological impacts. We used a mesocosm experiment to quantify the impact of eggs and larvae of the introduced cane toad (Bufo marinus) on fitness-related traits (number, size and time of emergence of metamorphs) of a native Australian frog species (Opisthodon ornatus). The results depended upon the timing of oviposition of the two taxa, and hence the life-history stages that came into contact. Growth and survival of O. ornatus tadpoles were enhanced when they preceded B. marinus tadpoles into ponds, and reduced when they followed B. marinus tadpoles into ponds, relative to when tadpoles of both species were added to ponds simultaneously. The dominant tadpole-tadpole interaction is competition, and the results are consistent with competitive priority effects. However, these priority effects were reduced or reversed when O. ornatus tadpoles encountered B. marinus eggs. Predation on toxic toad eggs reduced the survival of O. ornatus and B. marinus. The consequent reduction in tadpole densities allowed the remaining O. ornatus tadpoles to grow more rapidly and to metamorphose at larger body sizes (>60% disparity in mean mass). Thus, exposure to B. marinus eggs reduced the number of O. ornatus metamorphs, but increased their body sizes. If the increased size at metamorphosis more than compensates for the reduced survival, the effective reproductive output of native anurans may be increased rather than decreased by the invasive toad. Minor interspecific differences in the seasonal timing of oviposition thus have the potential to massively alter the impact of invasive cane toads on native anurans. 相似文献
76.
Kimotsuki T Niwa N Hicks MN Dunne M Cobbe SM Watanabe MA 《Journal of biological physics》2010,36(3):299-315
Roughly speaking, restitution is the dependence of recovery time of cardiac electrical activity on heart rate. Increased restitution
slope is theorized to be predictive of sudden death after heart injury such as from coronary artery occlusion (ischemia).
Adrenaline analogs are known to increase restitution slope in normal hearts, but their effects in failing hearts are unknown.
Twenty-six rabbits underwent coronary ligation (n = 15) or sham surgery (n = 11) and implantation of a lead in the heart for recording electrocardiograms. Eight weeks later, unanesthetized rabbits
were given 0.25–2.0 ml of 1 μmol/L isoprenaline intravenously, which increased heart rate. Heart rate was quantified by time
between QRS peaks (RR) and heart activity duration by R to T peak time (QTp). Ligated rabbits (n = 6) had lower ejection fraction than sham rabbits (n = 7, p < 0.0001) indicative of heart failure, but similar baseline RR (269 ± 15 vs 292 ± 23 ms, p = 0.07), QTp (104 ± 17 vs 91 ± 9 ms, p = 0.1), and isoprenaline-induced minimum RR (204 ± 11 vs 208 ± 6 ms, p = 0.4). The trajectory of QTp vs TQ plots displayed hysteresis and regions of negative slope. The slope of the positive slope
region was >1 in ligated rabbits (1.27 ± 0.66) and <1 in sham rabbits (0.35 ± 0.14, p = 0.004). The absolute value of the negative slope was greater in ligated rabbits (− 0.81 ± 0.52 vs − 0.35 ± 0.14, p = 0.04). Isoprenaline increased heart rate and slopes of restitution trajectory in failing hearts. The dynamics of restitution
trajectory may hold clues for sudden death in heart failure patients. 相似文献
77.
Peters RJ Bonnier HJ Bredee JJ 《International journal of cardiovascular interventions》1999,2(3):153-162
In 1996 the Minister of Public Health, Welfare and Sports in The Netherlands published a 'Planning Decree Special Interventions in the Heart'. She requested from the professional organizations guidelines for the indications for interventions in the heart. A working group was formed with representatives from the Dutch professional organizations for cardiology and thoracic surgery, to address this issue for patients with coronary artery disease. The working group confirmed the need to discuss all patients who are considered for either elective or emergency revascularization during a multidisciplinary consultation in (or with) one of the specialized Dutch hospitals. During this meeting of the 'heart team', at least one interventional cardiologist and one thoracic surgeon should be present. There are three possible outcomes of the heart team's consultations for each patient: drug therapy only ('conservative management'), coronary surgery or catheter intervention. For each case, the team should indicate the expected benefit, the risk of the intervention, the urgency and the estimated waiting time. The guidelines presented in this paper address these issues for three patient categories: stable angina pectoris, unstable angina pectoris and acute myocardial infarction. 相似文献
78.
We tested seven hypotheses regarding the mechanisms by which fluctuating asymmetry (FA) originates. We did this by analyzing data on four bilateral characters measured repeatedly during the development of individual domestic fowl. Immediately posthatching, there was substantial directional asymmetry, which rapidly decreased. We detected FA at significant levels in all characters in the majority of our measurements over the remainder of development. We also examined the effects of known environmental stressors (food and density stress) on levels of FA. At the levels we examined, changes in these stressors did not alter the degree of asymmetry we found in fowl. Time series of asymmetry for individuals did not exhibit regular oscillations, as much of the relevant literature predicts. Asymmetry levels reflected the combined effects of developmental noise, which was random in degree and direction, and feedback processes, which decreased asymmetry by altering growth rates on both sides of the body. Our findings best fit the predictions of the residual asymmetry and compensatory growth hypotheses, which suggest that levels of asymmetry reflect only recent growth history. 相似文献
79.
Michael J Christopher Zhi-Ping Chen Christian Rantzau Bruce E Kemp Frank P Alford 《Journal of applied physiology》2003,95(4):1523-1530
The effect of diabetes and exercise on skeletal muscle (SkM) AMP-activated protein kinase (AMPK)alpha1 and -alpha2 activities and site-specific phosphorylation of acetyl-CoA carboxylase was examined in the same six dogs before alloxan (35 mg/kg)-induced diabetes (C) and after 4-5 wk of suboptimally controlled hyperglycemic and hypoinsulinemic diabetes (DHG) in the presence and absence of 300-min phlorizin (50 microg.kg-1.min-1)-induced "normoglycemia" (DNG). In each study, the dog underwent a 150-min [3-3H]glucose infusion period, followed by a 30-min treadmill exercise test (60-70% maximal oxygen capacity) to measure the rate of glucose disposal into peripheral tissues (Rdtissue). SkM biopsies were taken from the thigh (vastus lateralis) before and immediately after exercise. In the C and DHG states, the rise in plasma free fatty acids (FFA) with exercise ( approximately 40%) was similar. In the DNG group, preexercise FFA were significantly higher, but the absolute rise in FFA with exercise was similar. However, the exercise-induced increment in Rdtissue was significantly blunted (by approximately 40-50%) in the DNG group compared with the other states. In SkM, preexercise AMPKalpha1 and -alpha2 activities were significantly elevated (by approximately 60-125%) in both diabetic states, but unlike the C group these activities did not rise further with exercise. Additionally, preexercise acetyl-CoA carboxylase phosphorylation in both diabetic states was elevated by approximately 70-80%, but the increases with exercise were similar to the C group. Preexercise AMPKalpha1 and -alpha2 activities were negatively correlated with Rdtissue during exercise for the combined groups (both P < 0.02). In conclusion, the elevated preexercise SkM AMPKalpha1 and -alpha2 activities contribute to the ongoing basal supply of glucose and fatty acid metabolism in suboptimally controlled hypoinsulinemic diabetic dogs; but whether they also play a permissive role in the metabolic stress response to exercise remains uncertain. 相似文献
80.