全文获取类型
收费全文 | 297篇 |
免费 | 26篇 |
出版年
2021年 | 5篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 11篇 |
2015年 | 10篇 |
2014年 | 12篇 |
2013年 | 16篇 |
2012年 | 18篇 |
2011年 | 13篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 15篇 |
2006年 | 10篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 8篇 |
2002年 | 2篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 11篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 7篇 |
1988年 | 10篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1982年 | 3篇 |
1979年 | 6篇 |
1978年 | 6篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1973年 | 5篇 |
1971年 | 4篇 |
1970年 | 6篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1967年 | 5篇 |
1966年 | 5篇 |
1965年 | 3篇 |
排序方式: 共有323条查询结果,搜索用时 31 毫秒
121.
122.
Applicability of Aeration and Delayed Addition of Selenite to the Isolation of Salmonellae 总被引:3,自引:2,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed. 相似文献
123.
124.
125.
126.
127.
Bennett Dyke Timothy B. Gage Patricia L. Alford Brent Swenson Sarah Williams-Blangero 《American journal of primatology》1995,37(1):25-37
Mortality statistics from three captive populations of chimpanzees (Pan troglodytes) were combined to generate standard model life tables for each sex in this species. The model is compared to an estimate of survivorship of a group of wild animals, and is applied to an incomplete data set to illustrate how the model may be used to extend estimates of mortality statistics to missing older ages. © 1995 Wiley-Liss, Inc. 相似文献
128.
129.
130.
Quantitative immunohistochemistry of synaptophysin in human neocortex: an alternative method to estimate density of presynaptic terminals in paraffin sections 总被引:15,自引:0,他引:15
E Masliah R D Terry M Alford R DeTeresa 《The journal of histochemistry and cytochemistry》1990,38(6):837-844
Currently available specific synaptic markers have made it possible to estimate the synaptic density by immunochemical techniques. In the present study we labeled the neocortical presynaptic terminals in histological sections of human autopsy tissue with a monoclonal antibody against synaptophysin. The characteristic granular neuropil reaction was quantified by measuring the average optical density (OD) in the different layers of the parietal cortex with the aid of image analysis equipment. The raw neuropil OD was corrected by subtracting the OD of the white matter in the same section. Our study showed that consistent microdensitometric results can be obtained on 5-microns paraffin sections from specimens with less than 8 hr of post-mortem time before fixation, incubated with 5 micrograms/ml of anti-synaptophysin. The corrected OD measurements were slightly larger in neocortical layers II, III, and V than in layers I, IV, and VI, but the differences were not statistically significant. In area 17, layer IV was denser than the others. We conclude that with certain precautions this method can be used to measure relative amounts of synaptophysin-like immunoreactivity and to infer the density of presynaptic boutons in human situations and in animal models. 相似文献