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151.
? Premise of the study: Seedling establishment and early survivorship are crucial steps for the regeneration of plant populations because both have long-lasting effects on plant population dynamics. For species recruiting through facilitation, species-specific facilitative effects might affect early fitness, an overlooked aspect in studies of facilitation considering groups of nurse species. ? Methods: We experimentally evaluated the roles of 10 nurse species and open space on the early performance of the columnar cactus Neobuxbaumia mezcalaensis. We measured establishment, survivorship, and growth of individuals over 3 years. Moreover, to study an extended period of the ontogeny of the interaction between this cactus and its nurse plants, we also monitored survivorship and growth rates of individuals between 3 to 12 cm tall during a 3-year period. ? Key results: Neobuxbaumia mezcalaensis performance varied significantly among nurse species, and only six yielded positive effects on early fitness. Densely canopied plants were the best nurses for this cactus. However, even among densely canopied species, some produced negative effects on the early fitness of N. mezcalaensis, indicating that similar nurse plants may elicit either facilitative or interference effects on beneficiary species. ? Conclusions: Our results emphasize the importance of species-specific facilitative interactions in the crucial early stages in the life cycle of N. mezcalaensis and how different nurse species modify the effect of seed-rain and contribute significantly to the population dynamics of the species. 相似文献
152.
Lucia Peluso Cristiana de Luca Silvia Bozza Antonio Leonardi Gloria Giovannini Alfonso Lavorgna Gaetano De Rosa Massimo Mascolo Loredana Ortega De Luna Maria Rosaria Catania Luigina Romani Fabio Rossano 《BMC microbiology》2010,10(1):1-11
Background
Porcine reproductive and respiratory syndrome (PRRS) has now been widely recognized as an economically important disease. The objective of this study was to compare the molecular and biological characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) field isolates in China to those of the modified live virus (MLV) PRRS vaccine and its parent strain (ATCC VR2332).Results
Five genes (GP2, GP3, GP4, GP5 and NSP2) of seven isolates of PRRSV from China, designated LS-4, HM-1, HQ-5, HQ-6, GC-2, GCH-3 and ST-7/2008, were sequenced and analyzed. Phylogenetic analyses based on the nucleotide sequence of the ORF2-5 and NSP2 showed that the seven Chinese isolates belonged to the same genetic subgroup and were related to the North American PRRSV genotype. Comparative analysis with the relevant sequences of another Chinese isolate (BJ-4) and North American (VR2332 and MLV) viruses revealed that these isolates have 80.8-92.9% homology with VR-2332, and 81.3-98.8% identity with MLV and 80.7-92.9% with BJ-4. All Nsp2 nonstructural protein of these seven isolates exhibited variations (a 29 amino acids deletion) in comparison with other North American PRRSV isolates. Therefore, these isolates were novel strain with unique amino acid composition. However, they all share more than 97% identity with other highly pathogenic Chinese PRRSV strains. Additionally, there are extensive amino acid (aa) mutations in the GP5 protein and the Nsp2 protein when compared with the previous isolates.Conclusions
These results might be useful to study the genetic diversity of PRRSV in China and to track the infection sources as well as for vaccines development. 相似文献153.
154.
Cantu A Ortega-S JA Mosqueda J Garcia-Vazquez Z Henke SE George JE 《Journal of wildlife diseases》2007,43(3):504-507
The suitability of white-tailed deer (Odocoileus virginianus) as hosts for the cattle ticks Rhipicephalus (Boophilus) microplus and Rhipicephalus (Boophilus) annulatus, has been well documented. These ticks have a wide host range, and both transmit Babesia bovis and Babesia bigemina, the agents responsible for bovine babesiosis. Although this disease and its vectors have been eradicated from the United States and some states in northern Mexico, it still is a problem in other Mexican states. It is not known if wild cervids like white-tailed deer can act as reservoirs for bovine babesiosis. The purpose of this study was to determine if B. bovis and B. bigemina or antibodies against them occur in white-tailed deer in the states of Nuevo Leon and Tamaulipas, Mexico. Twenty blood samples from white-tailed deer from two ranches were collected and tested with a nested polymerase chain reaction (nested PCR) and indirect immunofluorescence antibody test (IFAT) for B. bovis and B. bigemina. Eleven samples were positive for B. bigemina and four for B. bovis by nested PCR; amplicon sequences were identical to those reported in GenBank for B. bovis (Rap 1) and B. bigemina. Results of the IFA test showed the presence of specific antibodies in serum samples. This is the first report of the presence of B. bovis and B. bigemina in white-tailed deer using these techniques and underscores the importance of cervids as possible reservoirs for bovine babesiosis. 相似文献
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Evolution of hydraulic traits in closely related species pairs from Mediterranean and nonMediterranean environments of North America 总被引:2,自引:2,他引:0
Chaparral shrubs in California experience cool, wet winters and hot, dry summers characteristic of mediterranean-type climates; by contrast, morphologically similar close relatives in central Mexico experience summer rainfall. A comparison of closely related species pairs was conducted to examine whether evolutionary divergences in plant hydraulic conductivity were associated with contrasting seasonality of precipitation. Six species pairs in Santa Barbara, California and Tehuacan, Mexico were chosen to test for repeated directional divergences across the habitat contrast. Additionally, evolutionary correlations were examined using phylogenetically independent contrasts (PICs) among a suite of hydraulic traits, including stem- and leaf-specific conductivity, resistance to embolism, wood density, inverse Huber value, and minimum seasonal water potential. Leaf-specific conductivity was generally higher in California, but for most hydraulic traits the species pairs exhibited varied evolutionary trajectories across the climate contrast. A significant correlation was found between divergences in xylem resistance to embolism and minimum seasonal water potential, but no evolutionary trade-off was found between resistance and stem conductivity. Higher leaf-specific conductivity may be adaptive in California, where soil and atmospheric droughts coincide during summer months. This response is consistent with a hydraulic strategy of high leaf water supply under high evaporative demand to prevent excessive drops in water potential. 相似文献
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Mutations in HOXD13 underlie syndactyly type V and a novel brachydactyly-syndactyly syndrome
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Zhao X Sun M Zhao J Leyva JA Zhu H Yang W Zeng X Ao Y Liu Q Liu G Lo WH Jabs EW Amzel LM Shan X Zhang X 《American journal of human genetics》2007,80(2):361-371
HOXD13, the homeobox-containing gene located at the most 5' end of the HOXD cluster, plays a critical role in limb development. It has been shown that mutations in human HOXD13 can give rise to limb malformations, with variable expressivity and a wide spectrum of clinical manifestations. Polyalanine expansions in HOXD13 cause synpolydactyly, whereas amino acid substitutions in the homeodomain are associated with brachydactyly types D and E. We describe two large Han Chinese families with different limb malformations, one with syndactyly type V and the other with limb features overlapping brachydactyly types A4, D, and E and mild syndactyly of toes 2 and 3. Two-point linkage analysis showed LOD scores >3 (theta =0) for markers within and/or flanking the HOXD13 locus in both families. In the family with syndactyly type V, we identified a missense mutation in the HOXD13 homeodomain, c.950A-->G (p.Q317R), which leads to substitution of the highly conserved glutamine that is important for DNA-binding specificity and affinity. In the family with complex brachydactyly and syndactyly, we detected a deletion of 21 bp in the imperfect GCN (where N denotes A, C, G, or T) triplet-containing exon 1 of HOXD13, which results in a polyalanine contraction of seven residues. Moreover, we found that the mutant HOXD13 with the p.Q317R substitution was unable to transactivate the human EPHA7 promoter. Molecular modeling data supported these experimental results. The calculated interactions energies were in agreement with the measured changes of the activity. Our data established the link between HOXD13 and two additional limb phenotypes--syndactyly type V and brachydactyly type A4--and demonstrated that a polyalanine contraction in HOXD13, most likely, led to other digital anomalies but not to synpolydactyly. We suggest the term "HOXD13 limb morphopathies" for the spectrum of limb disorders caused by HOXD13 mutations. 相似文献
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