全文获取类型
收费全文 | 817篇 |
免费 | 58篇 |
专业分类
875篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 21篇 |
2020年 | 13篇 |
2019年 | 20篇 |
2018年 | 16篇 |
2017年 | 17篇 |
2016年 | 20篇 |
2015年 | 34篇 |
2014年 | 45篇 |
2013年 | 46篇 |
2012年 | 66篇 |
2011年 | 71篇 |
2010年 | 38篇 |
2009年 | 25篇 |
2008年 | 51篇 |
2007年 | 55篇 |
2006年 | 38篇 |
2005年 | 29篇 |
2004年 | 45篇 |
2003年 | 25篇 |
2002年 | 28篇 |
2001年 | 10篇 |
2000年 | 10篇 |
1999年 | 9篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 4篇 |
1994年 | 2篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 11篇 |
1981年 | 6篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1969年 | 3篇 |
1960年 | 3篇 |
1931年 | 1篇 |
排序方式: 共有875条查询结果,搜索用时 7 毫秒
831.
Nicholas J. Reichart Zackary J. Jay Viola Krukenberg Albert E. Parker Rachel L. Spietz Roland Hatzenpichler 《The ISME journal》2020,14(11):2851
Metagenomic studies have revolutionized our understanding of the metabolic potential of uncultured microorganisms in various ecosystems. However, many of these genomic predictions have yet to be experimentally tested, and the functional expression of genomic potential often remains unaddressed. In order to obtain a more thorough understanding of cell physiology, novel techniques capable of testing microbial metabolism under close to in situ conditions must be developed. Here, we provide a benchmark study to demonstrate that bioorthogonal non-canonical amino acid tagging (BONCAT) in combination with fluorescence-activated cell sorting (FACS) and 16S rRNA gene sequencing can be used to identify anabolically active members of a microbial community incubated in the presence of various growth substrates or under changing physicochemical conditions. We applied this approach to a hot spring sediment microbiome from Yellowstone National Park (Wyoming, USA) and identified several microbes that changed their activity levels in response to substrate addition, including uncultured members of the phyla Thaumarchaeota, Acidobacteria, and Fervidibacteria. Because shifts in activity in response to substrate amendment or headspace changes are indicative of microbial preferences for particular growth conditions, results from this and future BONCAT-FACS studies could inform the development of cultivation media to specifically enrich uncultured microbes. Most importantly, BONCAT-FACS is capable of providing information on the physiology of uncultured organisms at as close to in situ conditions as experimentally possible.Subject terms: Environmental microbiology, Microbial communities, Microbial ecology, Archaea 相似文献
832.
833.
834.
835.
836.
We describe a simple procedure to measure precisely the tip diameter of glass micropipets. This procedure can improve the precision of microinjection and some electrophysiological measurements due to their extreme sensitivity to the pipet's exact geometric dimensions. The technique is nondestructive and readily carried out as a brief, extra step in these applications. Digital measurements of the threshold pressures for gas bubbling from (gas filled) pipets submerged in methanol were used to calculate the corresponding inner tip diameters using the LaPlace equation. Direct measurements of the inner tip diameters (from 0.2-5.0 mu) using scanning electron microscopy confirmed the validity of this indirect, simple procedure. 相似文献
837.
838.
Performance of a Rapid Whole Blood Test for Helicobacter pylori in Primary Care: A German Multicenter Study 总被引:1,自引:0,他引:1
839.
840.
R E Viola R W Shaw S C Ransom J J Villafranca 《Archives of biochemistry and biophysics》1983,220(1):106-115
The interaction of solvent water protons with the bound paramagnetic metal ions of beef heart cytochrome c oxidase has been examined. The observed proton relaxation rates of enzyme solutions had a negative temperature dependence, indicating a rapid exchange between solvent protons in the coordination sphere of the metal ions and bulk solvent. An analysis of the dependence of the proton relaxation rate on the observation frequency indicated that the correlation time, which modulates the interaction between solvent protons and the unpaired electrons on the metal ions, is due to the electron spin relaxation time of the heme irons of cytochrome c oxidase. This means that at least one of the hemes is exposed to solvent. The proton relaxation rate of the oxidized enzyme was found to be sensitive to changes in ionic strength and to changes in the spin states of the metal ions. Heme a3 was found to be relatively inaccessible to bulk solvent. Partial reduction of the enzyme caused a slight increase in the relaxation rate, which may be due to a change in the antiferromagnetic coupling between two of the bound paramagnetic centers. Further reduction resulted in a decreased relaxation rate, and the fully reduced enzyme was no longer sensitive to changes in ionic strength. The binding of cytochrome c to cytochrome c oxidase had little effect on the proton relaxation rates of oxidized cytochrome oxidase indicating that cytochrome c binding has little effect on solvent accessibility to the metal ion sites. 相似文献