全文获取类型
收费全文 | 255篇 |
免费 | 31篇 |
出版年
2023年 | 2篇 |
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 9篇 |
2017年 | 16篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 20篇 |
2013年 | 13篇 |
2012年 | 20篇 |
2011年 | 25篇 |
2010年 | 13篇 |
2009年 | 13篇 |
2008年 | 19篇 |
2007年 | 10篇 |
2006年 | 11篇 |
2005年 | 11篇 |
2004年 | 12篇 |
2003年 | 7篇 |
2002年 | 13篇 |
2001年 | 7篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1972年 | 2篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1967年 | 1篇 |
排序方式: 共有286条查询结果,搜索用时 15 毫秒
261.
262.
263.
264.
265.
266.
267.
268.
Angélica E. Ramírez-Rodríguez Bernardo Bañuelos-Hernández Mariano J. García-Soto Dania G. Govea-Alonso Sergio Rosales-Mendoza M. Catalina Alfaro de la Torre 《International journal of phytoremediation》2019,21(7):617-623
AbstractArsenic contamination of groundwater is a significant problem in countries like Mexico, where San Luis Potosi is among the regions registering severe levels of it. Bioremediation with microalgae capable to absorb and metabolize metals or metalloids like arsenic reduces their toxicity and is a cost-effective approach compared to physical–chemical processes. We evaluated the capability of Chlamydomonas reinhardtii to remove arsenate and compared it with an acr3-modified recombinant strain, which we produced by transforming the wild-type strain with Agrobacterium tumefaciens using the construct pARR1 including a synthetic, optimized acr3 gene from Pteris vittata, a hyper-accumulator of arsenic. We monitored the growth of both strains in media with arsenate, containing a standard or a 10-fold decreased amount of phosphate. Comparing both strains in media initially with 0.5, 1, and 1.5?mg/L of arsenate, the acr3-modified strain removed 1.5 to 3 times more arsenic than the wild-type strain. Moreover, the arsenic uptake rate increased 1.2 to 2.3 times when growing the acr3-modified strain in media with decreased phosphate, while the uptake rate for the wild-type strain scarcely changed under the same conditions. These results confirm the expression of the acr3 gene in C. reinhardtii and its potential application to remove arsenic. 相似文献
269.
Felipe Bastida David J. Eldridge Sebastin Abades Fernando D. Alfaro Antonio Gallardo Laura García‐Velzquez Carlos García Stephen C. Hart Cecilia A. Prez Fernanda Santos Pankaj Trivedi Mark A. Williams Manuel Delgado‐Baquerizo 《Molecular ecology》2020,29(4):752-761
Unlike plants and vertebrates, the ecological preferences, and potential vulnerabilities of soil invertebrates to environmental change, remain poorly understood in terrestrial ecosystems globally. We conducted a cross‐biome survey including 83 locations across six continents to advance our understanding of the ecological preferences and vulnerabilities of the diversity of dominant and functionally important soil invertebrate taxa, including nematodes, arachnids and rotifers. The diversity of invertebrates was analyzed through amplicon sequencing. Vegetation and climate drove the diversity and dominant taxa of soil invertebrates. Our results suggest that declines in forest cover and plant diversity, and reductions in plant production associated with increases in aridity, can result in reductions of the diversity of soil invertebrates in a drier and more managed world. We further developed global atlases of the diversity of these important soil invertebrates, which were cross‐validated using an independent database. Our study advances the current knowledge of the ecological preferences and vulnerabilities of the diversity and presence of functionally important soil invertebrates in soils from across the globe. This information is fundamental for improving and prioritizing conservation efforts of soil genetic resources and management policies. 相似文献
270.
R. Eduardo Palma Dusan Boric‐Bargetto J. Pablo Jayat David A. Flores Horacio Zeballos Víctor Pacheco Ricardo A. Cancino Fernando D. Alfaro Enrique Rodríguez‐Serrano Ulyses F. J. Pardiñas 《Zoologica scripta》2014,43(3):217-234
The mouse opossums of the genus Thylamys constitute a group of species mainly adapted to open xeric‐like habitats and restricted to the southern portion of South America. We used molecular data (mitochondrial and nuclear sequences) to evaluate the phylogenetic and biogeographical relationships of all currently known living species of the genus, recognizing a new taxon from the middle and high elevations of the Peruvian Andes and evaluating the phylogenetic structuring within T. pallidior and T. elegans, as well as the validity of T. sponsorius, T. cinderella and T. tatei, and the haplogroups recognized within T. pusillus. Our results confirm the monophyly of the genus and that the Caatinga and the Cerrado inhabitants Thylamys karimii and T. velutinus are the most basal species in the radiation of Thylamys. We also calibrated a molecular clock which hypothesized a time of origin of the genus of about 24 My, with most species differentiating in middle and late Miocene and Plio‐Pleistocene times of South America. 相似文献