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M. V. Stanovova P. A. Erokhov N. G. Gornostaev V. S. Mikhailov Yu. V. Lyupina 《Doklady. Biochemistry and biophysics》2016,471(1):428-430
We investigated functioning of proteasomes and chaperones in Arenicola marina coelomocytes in conditions of lipopolysaccharide-induced inflammation. We observed the increase of chymotrypsin-like proteasome activity in coelomocytes 1 h after induction. Amount of proteasome subunits alpha- and beta-5 types increased as well. We also detected appearance of a new form of Hsp70 chaperone in infected coelomocytes. Our results allow us to consider the changes in proteasome structure and induction of chaperones as principle mechanisms in stress adaptation and defensive reactions development in annelids. 相似文献
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Liquid‐like Ionic Conduction in Solid Lithium and Sodium Monocarba‐closo‐decaborates near or at Room Temperature
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Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen,Legionella pneumophila
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Peter J Stogios Mariya Morar Chitong Rao Rosa Di Leo Elena Evdokimova Mandy Lam Christina Oatway Marianne E Cuff Jerzy Osipiuk Karolina Michalska Boguslaw P Nocek Mikko Taipale Alexei Savchenko Alexander W Ensminger 《Molecular systems biology》2016,12(12)
Pathogens deliver complex arsenals of translocated effector proteins to host cells during infection, but the extent to which these proteins are regulated once inside the eukaryotic cell remains poorly defined. Among all bacterial pathogens, Legionella pneumophila maintains the largest known set of translocated substrates, delivering over 300 proteins to the host cell via its Type IVB, Icm/Dot translocation system. Backed by a few notable examples of effector–effector regulation in L. pneumophila, we sought to define the extent of this phenomenon through a systematic analysis of effector–effector functional interaction. We used Saccharomyces cerevisiae, an established proxy for the eukaryotic host, to query > 108,000 pairwise genetic interactions between two compatible expression libraries of ~330 L. pneumophila‐translocated substrates. While capturing all known examples of effector–effector suppression, we identify fourteen novel translocated substrates that suppress the activity of other bacterial effectors and one pair with synergistic activities. In at least nine instances, this regulation is direct—a hallmark of an emerging class of proteins called metaeffectors, or “effectors of effectors”. Through detailed structural and functional analysis, we show that metaeffector activity derives from a diverse range of mechanisms, shapes evolution, and can be used to reveal important aspects of each cognate effector's function. Metaeffectors, along with other, indirect, forms of effector–effector modulation, may be a common feature of many intracellular pathogens—with unrealized potential to inform our understanding of how pathogens regulate their interactions with the host cell. 相似文献
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V. I. Chikov A. L. Mikhailov O. A. Timofeeva L. A. Khamidullina 《Russian Journal of Plant Physiology》2016,63(1):70-76
Photosynthetic assimilation of 14CO2 was examined in leaves of potato (Solanum tuberosum L.) plants that were grown under direct sunlight and then transferred to 50% irradiance for various periods. The rate of 14CO2 assimilation correlated with light intensity: the photosynthetic rate reduced by 52% after 5-day shading and by 70% after 30-min shading. In all shaded and shade-adapted plants, the sucrose/hexose ratio decreased by a factor of 3.5–4.1; furthermore, the radioactivity of glycolate cycle metabolites and the serine/glycine ratio were lowered. In plants shaded for 5 days or 30 min, the radioactivity of aspartate and malate was higher than at continuous high irradiance, especially in plants shaded for 30 min, whereas a sudden illumination of the shaded plants reduced the radioactivity of these substances. We suppose that low irradiance averted the reentry of glycolate path carbon into the Calvin cycle and redirected this carbon source for the production of four-carbon acids that acidified the apoplast. This acidification activated the apoplastic invertase, which enhanced sucrose hydrolysis and hindered the sucrose export from the leaf. Hydrolysis of sucrose promoted the increase in osmolarity of the apoplastic solution, this increase being stronger at close distances to the stomatal pores where water is intensely evaporated. The increase in osmolarity of extracellular medium led to closing of stomata and the suppression of photosynthesis. 相似文献
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A. K. Dulatov P. S. Krapiva B. D. Lemeshko Yu. V. Mikhailov I. N. Moskalenko I. A. Prokuratov A. N. Selifanov 《Plasma Physics Reports》2016,42(1):38-44
The process of hard X-ray (HXR) generation in plasma focus (PF) chambers was studied experimentally. The radiation was recorded using scintillation detectors with a high time resolution and thermoluminescent detectors in combination with the method of absorbing filters. Time-resolved analysis of the processes of neutron and X-ray generation in PFs is performed. The spectra of HXR emission from PF chambers with deuterium and deuterium–tritium fillings are determined. In experiments with PF chambers filled with a deuterium–tritium mixture, in addition to the HXR pulse with photon energies of up to 200–300 keV, a γ-ray pulse with photon energies of up to 2.5–3.0 MeV is recorded, and a mechanism of its generation is proposed. 相似文献
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Clemens Benjamin J. Weitkamp Laurie Siwicke Kevin Wade Joy Harris Julianne Hess Jon Porter Laurie Parker Keith Sutton Trent Orlov Alexei M. 《Reviews in Fish Biology and Fisheries》2019,29(4):767-788
Reviews in Fish Biology and Fisheries - Pacific lamprey Entosphenus tridentatus has an anadromous life cycle that begins with larvae that filter-feed in freshwater, followed by transformation into... 相似文献
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