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981.
Biros SM Moisan L Mann E Carella A Zhai D Reed JC Rebek J 《Bioorganic & medicinal chemistry letters》2007,17(16):4641-4645
The design and synthesis of alpha-helix peptidomimetics using inverse electron demand Diels-Alder reactions is described. The potency of the resulting pyridazine-based library to disrupt the Bak/Bcl-X(L) interaction was tested using an in vitro fluorescence polarization assay. 相似文献
982.
Review paper on current technologies for decolourisation of textile wastewaters: perspectives for anaerobic biotechnology 总被引:19,自引:0,他引:19
Dyes are natural and xenobiotic compounds that make the world more beautiful through coloured substances. However, the release of coloured wastewaters represents a serious environmental problem and a public health concern. Colour removal, especially from textile wastewaters, has been a big challenge over the last decades, and up to now there is no single and economically attractive treatment that can effectively decolourise dyes. In the passed years, notable achievements were made in the use of biotechnological applications to textile wastewaters not only for colour removal but also for the complete mineralization of dyes. Different microorganisms such as aerobic and anaerobic bacteria, fungi and actinomycetes have been found to catalyse dye decolourisation. Moreover, promising results were obtained in accelerating dye decolourisation by adding mediating compounds and/or changing process conditions to high temperatures. This paper provides a critical review on the current technologies available for decolourisation of textile wastewaters and it suggests effective and economically attractive alternatives. 相似文献
983.
Gomes A Fernandes E Silva AM Santos CM Pinto DC Cavaleiro JA Lima JL 《Bioorganic & medicinal chemistry》2007,15(18):6027-6036
2-Styrylchromones are a small group of naturally occurring chromones, vinylogues of flavones (2-phenylchromones). Natural and synthetic 2-styrylchromones have been tested in different biological systems, showing activities with potential therapeutic applications. In particular, the potential and hitherto understudied antioxidant behavior of these compounds has been raised as a matter of interest. Thus the present work consisted in the study of the in vitro scavenging activities for reactive oxygen species (ROS) and reactive nitrogen species (RNS) of various 2-styrylchromone derivatives and structurally similar flavonoids. Some of the studied 2-styrylchromones proved to be extremely efficient scavengers of the different ROS and RNS, showing, in some cases, IC(50)s under 1 microM. The hydroxylation pattern of 2-styrylchromones, especially in the B-ring but also in the A ring, modulates the activity of these compounds, the catecholic derivatives being the most effective scavengers. The styryl pattern also contributes to their observed outstanding antioxidant activity. In conclusion, the scavenging activities for ROS/RNS of 2-styrylchromone derivatives, here shown for the first time, provide novel and most promising compounds to be applied as antioxidants. 相似文献
984.
985.
Gil-Brusola A Pemán J Santos M Salavert M Lacruz J Gobernado M 《Revista iberoamericana de micología》2007,24(4):312-316
We report a case of disseminated histoplasmosis in a 33-year old Ecuadorian patient with AIDS and a CD4 lymphocyte count of 39 cells/microl. He presented with prolonged fever and cough, was diagnosed with hemophagocytic syndrome and multiple organ failure and died 18 days after admission. Histoplasma capsulatum was isolated post-mortem from bone marrow biopsy and blood culture. In a literature review we found 22 published cases of disseminated histoplasmosis in patients with AIDS in Spain since 1988. All but two were men under 50 years old. Nineteen had been born or had lived in endemic areas. The diagnosis of histoplasmosis was established by culture of bone marrow biopsy in 10 cases. Itraconazole was introduced as a second drug after amphotericin B in ten of the thirteen patients who survived. 相似文献
986.
Human TH17 lymphocytes promote blood-brain barrier disruption and central nervous system inflammation 总被引:23,自引:0,他引:23
Kebir H Kreymborg K Ifergan I Dodelet-Devillers A Cayrol R Bernard M Giuliani F Arbour N Becher B Prat A 《Nature medicine》2007,13(10):1173-1175
T(H)17 lymphocytes appear to be essential in the pathogenesis of numerous inflammatory diseases. We demonstrate here the expression of IL-17 and IL-22 receptors on blood-brain barrier endothelial cells (BBB-ECs) in multiple sclerosis lesions, and show that IL-17 and IL-22 disrupt BBB tight junctions in vitro and in vivo. Furthermore, T(H)17 lymphocytes transmigrate efficiently across BBB-ECs, highly express granzyme B, kill human neurons and promote central nervous system inflammation through CD4+ lymphocyte recruitment. 相似文献
987.
Coelho LG Bastos EM Resende CC Paula e Silva CM Sanches BS de Castro FJ Moretzsohn LD Vieira WL Trindade OR 《Helicobacter》2007,12(5):572-574
Recent in vitro studies suggest that propolis and some of its phenolic components are able to inhibit Helicobacter pylori growth. To date, there are no clinical studies. AIMS: To evaluate the effect of Brazilian green propolis on H. pylori-infected individuals. PATIENTS AND METHODS: Eighteen (11 females, 7 males, mean age 47 years) participants were included. Before treatment, all participants were submitted to gastroscopy, and H. pylori infection was confirmed by histology, urease test, and (13)C-urea breath test (UBT). Participants with UBT showing a delta over baseline (DOB) value higher than 4 per thousand were considered positive for H. pylori infection. Twenty drops from an alcoholic preparation of Brazilian green propolis were administered three times a day for 7 days. Clinical evaluation and UBT were performed at 1-3 days and at 40 days after the end of therapy to evaluate H. pylori suppression or eradication, respectively. RESULTS: All participants took all medication and completed the study. Eighty-three percent of the subjects did not succeed in suppressing or eradicating H. pylori. Two participants reached partial suppression after treatment, but became positive again at UBT performed 40 days after treatment. Another participant presented negative at UBT 40 days after treatment, not confirmed by a second UBT performed 100 days after treatment. CONCLUSIONS: Brazilian green propolis used in popular dose showed minimal effect on H. pylori infection. Larger studies with longer duration, larger dose, and different frequency of administration of propolis extract should be undertaken to define its role on H. pylori therapy. 相似文献
988.
Gisbert JP Calvet X Feu F Bory F Cosme A Almela P Santolaria S Aznárez R Castro M Fernández N García-Grávalos R Cañete N Benages A Montoro M Borda F Pérez-Aisa A Piqué JM 《Helicobacter》2007,12(4):279-286
AIM: To evaluate the effect of Helicobacter pylori eradication on ulcer bleeding recurrence in a prospective, long-term study including more than 400 patients. METHODS: Patients with peptic ulcer bleeding were prospectively included. H. pylori infection was confirmed by rapid urease test, histology or (13)C-urea breath test. Several eradication regimens were used. Ranitidine 150 mg was administered daily until eradication was confirmed by breath test 8 weeks after completing eradication therapy. Patients with therapy failure received a second or third course of therapy. Patients with eradication success did not receive maintenance anti-ulcer therapy, and were controlled yearly with a repeated breath test. RESULTS: Four hundred and twenty-two patients were followed up for at least 12 months, with a total of 906 patient-years of follow up. Mean age was 59 years, and 35% were previous nonsteroidal anti-inflammatory drug (NSAID) users. Sixty-nine percent had duodenal, 24% gastric, and 7% pyloric ulcer. Recurrence of bleeding was demonstrated in two patients at 1 year (incidence: 0.22% per patient-year of follow up), which occurred after NSAID use in both cases. CONCLUSION: Peptic ulcer rebleeding does not occur in patients with complicated ulcers after H. pylori eradication. Maintenance anti-ulcer (antisecretory) therapy is not necessary if eradication is achieved. 相似文献
989.
Santos X Arenas C Llorente GA Ruiz X 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2007,147(1):165-172
Reptiles are typical capital breeders that fuel reproduction by the use of lipids stored in fat bodies. However, little is known about the origin (exogenous or endogenous) of egg protein. We have examined the origin of egg protein by means of the analysis of protein content of liver and carcass (skeletal muscle) of the oviparous snake Natrix maura throughout an annual cycle. We have also measured monthly variation of the digestive-content mass and the ovarian mass. Results showed that protein in liver peaked during vitellogenesis according to the role of the liver in the synthesis of vitellogenin. Partial correlations showed the path of protein from the prey (digestive-content) to the liver, and finally to the ovaries, as well as an inverse relation between carcass protein and ovarian mass. Carcass muscle is the only body part that may act as a potential reserve for endogenous protein, although we did not find significant variation in carcass protein during female reproduction. As females with large follicles did not stop foraging activity, we assumed that egg protein was derived from the diet as partial correlations indicated. Our results suggest that N. maura is a capital breeder for lipids and tend to be income breeder for protein. This conclusion contrasts with that observed in capital breeders for which egg protein was derived from muscle. We discuss the idea that flexibility in the origin of egg protein could affect the body condition in post-reproductive females. 相似文献
990.
Liang AM Claret E Ouled-Diaf J Jean A Vogel D Light DR Jones SW Guilford WJ Parkinson JF Snider RM 《Journal of biomolecular screening》2007,12(4):536-545
Leukotriene A4 (LTA4) hydrolase catalyzes a rate-limiting final biosynthetic step of leukotriene B4 (LTB4), a potent lipid chemotactic agent and proinflammatory mediator. LTB4 has been implicated in the pathogenesis of various acute and chronic inflammatory diseases, and thus LTA4 hydrolase is regarded as an attractive therapeutic target for anti-inflammation. To facilitate identification and optimization of LTA4 hydrolase inhibitors, a specific and efficient assay to quantify LTB4 is essential. This article describes the development of a novel 384-well homogeneous time-resolved fluorescence assay for LTB4 (LTB4 HTRF assay) and its application to establish an HTRF-based LTA4 hydrolase assay for lead optimization. This LTB4 HTRF assay is based on competitive inhibition and was established by optimizing the reagent concentration, buffer composition, incubation time, and assay miniaturization. The optimized assay is sensitive, selective, and robust, with a Z' factor of 0.89 and a subnanomolar detection limit for LTB4. By coupling this LTB4 HTRF assay to the LTA4 hydrolase reaction, an HTRF-based LTA4 hydrolase assay was established and validated. Using a test set of 16 LTA4 hydrolase inhibitors, a good correlation was found between the IC50 values obtained using LTB4 HTRF with those determined using the LTB enzyme-linked immunoassay (R = 0.84). The HTRF-based LTA4 hydrolase assay was shown to be an efficient and suitable assay for determining compound potency and library screening to guide the development of potent inhibitors of LTA4 hydrolase. 相似文献