全文获取类型
收费全文 | 5188篇 |
免费 | 411篇 |
国内免费 | 1篇 |
出版年
2024年 | 5篇 |
2023年 | 35篇 |
2022年 | 81篇 |
2021年 | 185篇 |
2020年 | 115篇 |
2019年 | 156篇 |
2018年 | 149篇 |
2017年 | 157篇 |
2016年 | 220篇 |
2015年 | 329篇 |
2014年 | 325篇 |
2013年 | 387篇 |
2012年 | 448篇 |
2011年 | 448篇 |
2010年 | 264篇 |
2009年 | 239篇 |
2008年 | 300篇 |
2007年 | 299篇 |
2006年 | 283篇 |
2005年 | 247篇 |
2004年 | 195篇 |
2003年 | 176篇 |
2002年 | 154篇 |
2001年 | 49篇 |
2000年 | 38篇 |
1999年 | 43篇 |
1998年 | 39篇 |
1997年 | 10篇 |
1996年 | 12篇 |
1995年 | 9篇 |
1994年 | 17篇 |
1993年 | 7篇 |
1992年 | 13篇 |
1991年 | 19篇 |
1990年 | 10篇 |
1989年 | 13篇 |
1988年 | 10篇 |
1987年 | 8篇 |
1986年 | 12篇 |
1985年 | 9篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 9篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1975年 | 9篇 |
1974年 | 6篇 |
1971年 | 7篇 |
1970年 | 3篇 |
排序方式: 共有5600条查询结果,搜索用时 31 毫秒
991.
992.
Alexandre Esadze Catherine A. Kemme Anatoly B. Kolomeisky Junji Iwahara 《Nucleic acids research》2014,42(11):7039-7046
993.
Cristina D. Castilhos Jamila MezzaliraMariana P.S. Goldim Vanessa V. DaitxCristina da S. Garcia Carla V. AndradeAna C. Breier Jaqueline CéAlexandre S. Mello Janice C. Coelho 《Gene》2014
This study aimed to determine the enzymatic activity in dried blood samples collected on filter paper (DBS) for the diagnosis of the following diseases: Fabry, Pompe, Mucopolysaccharidosis type I (MPS I) and Mucopolysaccharosis type VI (MPS VI). DBS was used for high risk patientscreening, according to clinical suspicion. Plasma, leukocytes and cultured fibroblasts were used to confirm the diagnosis when necessary. Among the 529 DBS samples sent to the laboratory, 164 had abnormal results. Confirmatory materials of 73 individuals were rerouted. The frequency of diagnosis for lysosomal storage disorders was 5.9%. DBS is an alternative screening technique used in high risk populations, which should lead to earlier diagnosis for lysosomal storage disorders (LSDs), help patients get treatment sooner and improve the outcome of the disease. 相似文献
994.
Lo?c Etienne Fran?ois Rousset Bernard Godelle Alexandre Courtiol 《Proceedings. Biological sciences / The Royal Society》2014,281(1785)
Most theoretical research in sexual selection has focused on indirect selection. However, empirical studies have not strongly supported indirect selection. A well-established finding is that direct benefits and costs exert a strong influence on the evolution of mate choice. We present an analytical model in which unilateral mate choice evolves solely by direct sexual selection on choosiness. We show this is sufficient to generate the evolution of all possible levels of choosiness, because of the fundamental trade-off between mating rate and mating benefits. We further identify the relative searching time (RST, i.e. the proportion of lifetime devoted to searching for mates) as a predictor of the effect of any variable affecting the mating rate on the evolution of choosiness. We show that the RST: (i) allows one to make predictions about the evolution of choosiness across a wide variety of mating systems; (ii) encompasses all alternative variables proposed thus far to explain the evolution of choosiness by direct sexual selection; and (iii) can be empirically used to infer qualitative differences in choosiness. 相似文献
995.
Jean-Pierre Rospars Alexandre Grémiaux David Jarriault Antoine Chaffiol Christelle Monsempes Nina Deisig Sylvia Anton Philippe Lucas Dominique Martinez 《PLoS computational biology》2014,10(12)
In the olfactory system of male moths, a specialized subset of neurons detects and processes the main component of the sex pheromone emitted by females. It is composed of several thousand first-order olfactory receptor neurons (ORNs), all expressing the same pheromone receptor, that contact synaptically a few tens of second-order projection neurons (PNs) within a single restricted brain area. The functional simplicity of this system makes it a favorable model for studying the factors that contribute to its exquisite sensitivity and speed. Sensory information—primarily the identity and intensity of the stimulus—is encoded as the firing rate of the action potentials, and possibly as the latency of the neuron response. We found that over all their dynamic range, PNs respond with a shorter latency and a higher firing rate than most ORNs. Modelling showed that the increased sensitivity of PNs can be explained by the ORN-to-PN convergent architecture alone, whereas their faster response also requires cell-to-cell heterogeneity of the ORN population. So, far from being detrimental to signal detection, the ORN heterogeneity is exploited by PNs, and results in two different schemes of population coding based either on the response of a few extreme neurons (latency) or on the average response of many (firing rate). Moreover, ORN-to-PN transformations are linear for latency and nonlinear for firing rate, suggesting that latency could be involved in concentration-invariant coding of the pheromone blend and that sensitivity at low concentrations is achieved at the expense of precise encoding at high concentrations. 相似文献
996.
997.
Cristina Baldauf Maísa Ciampi-Guillardi Thaísa Jacinto Aguirra Christiane Erondina Corrêa Flavio Antonio Maës dos Santos Anete Pereira de Souza Alexandre Magno Sebbenn 《Conservation Genetics》2014,15(5):1073-1083
We assessed the pollen and seed dispersal patterns, genetic diversity, inbreeding and spatial genetic structure of Himatanthus drasticus (Apocynaceae), a tree native to the Brazilian Savanna (Cerrado) that is heavily exploited for its medicinal latex. The study was conducted in the Araripe National Forest, Ceará State, Brazil. Within a one-hectare plot, samples were collected from all adult trees, adult trees located in the immediate vicinity of the plot, and seedlings. All sampled individuals were mapped and genotyped using microsatellite markers. High levels of polymorphism and significant levels of inbreeding were found, which indicates that self-fertilisation and mating among relatives occur in this population. Both the adults and seedlings had significant spatial genetic structure up to ~40 m and our results confirmed the occurrence of isolation by distance. Pollen and seeds were dispersed over short distances and immigration of pollen and seeds into the plot was estimated at 13 and 9 %, respectively. Taking into consideration the degree of inbreeding, relatedness, intrapopulation spatial genetic structure and pollen dispersal distance, we recommend collecting seeds from a large number of trees spaced at least 150 m apart to avoid collecting seeds from related individuals and an overlap of pollen pools among seed trees. 相似文献
998.
999.
A. E. Ruiz‐Contreras K. Carrillo‐Sánchez I. Ortega‐Mora M. A. Barrera‐Tlapa T. V. Román‐López C. B. Rosas‐Escobar L. Flores‐Barrera U. Caballero‐Sánchez Z. Muñoz‐Torres S. Romero‐Hidalgo S. Hernández‐Morales J. A. González‐Barrios F. Vadillo‐Ortega M. Méndez‐Díaz R. Aguilar‐Roblero O. Prospéro‐García 《Genes, Brain & Behavior》2014,13(2):173-178
Individual differences in cognitive performance are partly dependent, on genetic polymporhisms. One of the single‐nucleotide polymorphisms (SNP) of the CNR1 gene, which codes for cannabinoid receptor 1 (CB1R), is the rs2180619, located in a regulatory region of this gene (6q14–q15). The alleles of the rs2180619 are A > G; the G allele has been associated with addiction and high levels of anxiety (when the G allele interacts with the SS genotype of the 5‐HTTLPR gene). However, GG genotype is observed also in healthy subjects. Considering G allele as risk for ‘psychopathological conditions’, it is possible that GG healthy subjects do not be addicted or anxious, but would have reduced performance, compared to AA subjects, in attentional control and working memory processing. One hundred and sixty‐four healthy young Mexican‐Mestizo subjects (100 women and 64, men; mean age: 22.86 years, SD=2.72) participated in this study, solving a task where attentional control and working memory were required. GG subjects, compared to AA subjects showed: (1) a general lower performance in the task (P = 0.02); (2) lower performance only when a high load of information was held in working memory (P = 0.02); and (3) a higher vulnerability to distractors (P = 0.03). Our results suggest that, although the performance of GG subjects was at normal levels, a lower efficiency of the endocannabinoid system, probably due to a lowered expression of CB1R, produced a reduction in the performance of these subjects when attentional control and working memory processing is challenged . 相似文献
1000.
Maria das Neves Paiva-Cardoso Francisco Morinha Paulo Barros Hélia Vale-Gonçalves Ana Cláudia Coelho Lisete Fernandes Paulo Travassos Ana Sofia Faria Estela Bastos Mário Santos João Alexandre Cabral 《European Journal of Wildlife Research》2014,60(4):645-649
The psychrophilic fungus Pseudogymnoascus destructans (formerly known as Geomyces destructans) is considered the etiological agent of white-nose disease (WND), an emerging disease which affects bats during their hibernation period. This disease is clinically characterized by the growth of a white fungus on muzzle, ears, and wings’ membranes of affected bats. This infection caused the death of several million bats in North America. Conversely, European bats show no evidence of significant mortality occurrences associated with P. destructans colonization. This fungus has been isolated from bats in at least 15 European countries since 2008, but was never before reported in the Iberian Peninsula. This study describes the first case report of P. destructans colonization in bats from Portugal. We isolated P. destructans from three hibernating Myotis blythii (lesser mouse-eared bat) with visual signs of P. destructans colonization, during a routine visit to a mine located in the Trás-os-Montes region, Northern Portugal. M. blythii is one of the rarest bat species in Europe, classified as critically endangered in Portugal. P. destructans was obtained from at least three different parts of the body of each specimen analyzed. The identification of the respective fungal isolates was based on the macroscopic and microscopic characterization of the cultures and confirmed by PCR-based analysis. All nucleotide sequences obtained showed 100 % identity with previous data reported for P. destructans. This new finding improves the current knowledge about the European distribution of P. destructans, which is of great interest for forthcoming studies on the fungus dispersion and impact among bat populations at regional and/or global level. 相似文献