全文获取类型
收费全文 | 17774篇 |
免费 | 1168篇 |
国内免费 | 6篇 |
专业分类
18948篇 |
出版年
2023年 | 111篇 |
2022年 | 149篇 |
2021年 | 332篇 |
2020年 | 212篇 |
2019年 | 280篇 |
2018年 | 394篇 |
2017年 | 351篇 |
2016年 | 532篇 |
2015年 | 816篇 |
2014年 | 797篇 |
2013年 | 1042篇 |
2012年 | 1470篇 |
2011年 | 1943篇 |
2010年 | 1039篇 |
2009年 | 986篇 |
2008年 | 937篇 |
2007年 | 888篇 |
2006年 | 844篇 |
2005年 | 741篇 |
2004年 | 709篇 |
2003年 | 657篇 |
2002年 | 586篇 |
2001年 | 242篇 |
2000年 | 191篇 |
1999年 | 188篇 |
1998年 | 132篇 |
1997年 | 94篇 |
1996年 | 73篇 |
1995年 | 83篇 |
1994年 | 65篇 |
1993年 | 61篇 |
1992年 | 102篇 |
1991年 | 80篇 |
1990年 | 96篇 |
1989年 | 104篇 |
1988年 | 91篇 |
1987年 | 63篇 |
1986年 | 87篇 |
1985年 | 98篇 |
1983年 | 62篇 |
1982年 | 55篇 |
1981年 | 57篇 |
1979年 | 85篇 |
1978年 | 54篇 |
1974年 | 80篇 |
1973年 | 81篇 |
1970年 | 71篇 |
1969年 | 65篇 |
1968年 | 61篇 |
1967年 | 59篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
11.
Daneen Schaeffer Filipa Pereira Reis Sean J. Johnson Cec��lia Maria Arraiano Ambro van Hoof 《Nucleic acids research》2012,40(18):9298-9307
The 10-subunit RNA exosome is involved in a large number of diverse RNA processing and degradation events in eukaryotes. These reactions are carried out by the single catalytic subunit, Rrp44p/Dis3p, which is composed of three parts that are conserved throughout eukaryotes. The exosome is named for the 3′ to 5′ exoribonuclease activity provided by a large C-terminal region of the Rrp44p subunit that resembles other exoribonucleases. Rrp44p also contains an endoribonuclease domain. Finally, the very N-terminus of Rrp44p contains three Cys residues (CR3 motif) that are conserved in many eukaryotes but have no known function. These three conserved Cys residues cluster with a previously unrecognized conserved His residue in what resembles a metal-ion-binding site. Genetic and biochemical data show that this CR3 motif affects both endo- and exonuclease activity in vivo and both the nuclear and cytoplasmic exosome, as well as the ability of Rrp44p to associate with the other exosome subunits. These data provide the first direct evidence that the exosome-Rrp44p interaction is functionally important and also provides a molecular explanation for the functional defects when the conserved Cys residues are mutated. 相似文献
12.
Drahomíra Kenov Lucie oltysov Michal Pravenec Marie-Pierre Moisan W. Theodore Kurtz Vladimír Ken 《Journal of Experimental Animal Science》2000,41(1-2)
The SHR-Lx congenic strain carrying a differential segment of chromosome 8 of BN and PD origin was recently shown to exhibit a significant decrease in blood pressure as compared to the SHR strain. There were two positional candidate genes for blood pressure control mapped to the differential segment: the rat kidney epithelial potassium channel gene (Kcnj1) and brain dopamine receptor 2 gene (Drd2). Bot these genes were separated into SHR.BN-RNO8 congenic substrains. In this communication, we are presenting the assignment of two further putative candidate genes, which might be involved in blood pressure control to the BN/PD differential segment of the SHR-Lx congenic strain. These are: the gene coding for smooth muscle cell specific protein 22 (Sm22) defined by the D8Mcw1 marker and neuronal nicotinic acetylcholine receptor gene cluster, defined by the D8Bord1 marker. Moreover, the glutamate receptor gene Grik4 which also maps to the differential segment of the SHR-Lx should be taken into account. The genetic separation of all these putative candidate genes of blood pressure control is being performed by recombinations and subsequent selection using (SHR×SHR-Lx) intercross population. 相似文献
13.
14.
F. Šrámek 《Biologia Plantarum》1999,42(4):582-582
15.
Rita Padányi Yuning Xiong Géza Antalffy Krisztina Lór Katalin Pászty Emanuel E. Strehler ágnes Enyedi 《The Journal of biological chemistry》2010,285(41):31704-31712
The membrane localization of the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) in polarized cells is determined by alternative splicing; the PMCA2w/b splice variant shows apical localization, whereas the PMCA2z/b and PMCA2x/b variants are mostly basolateral. We previously reported that PMCA2b interacts with the PDZ protein Na+/H+ exchanger regulatory factor 2 (NHERF2), but the role of this interaction for the specific membrane localization of PMCA2 is not known. Here we show that co-expression of NHERF2 greatly enhanced the apical localization of GFP-tagged PMCA2w/b in polarized Madin-Darby canine kidney cells. GFP-PMCA2z/b was also redirected to the apical membrane by NHERF2, whereas GFP-PMCA2x/b remained exclusively basolateral. In the presence of NHERF2, GFP-PMCA2w/b co-localized with the actin-binding protein ezrin even after disruption of the actin cytoskeleton by cytochalasin D or latrunculin B. Surface biotinylation and fluorescence recovery after photobleaching experiments demonstrated that NHERF2-mediated anchorage to the actin cytoskeleton reduced internalization and lateral mobility of the pump. Our results show that the specific interaction with NHERF2 enhances the apical concentration of PMCA2w/b by anchoring the pump to the apical membrane cytoskeleton. The data also suggest that the x/b splice form of PMCA2 contains a dominant lateral targeting signal, whereas the targeting and localization of the z/b form are more flexible and not fully determined by intrinsic sequence features. 相似文献
16.
Photoelectric signals from dried oriented purple membranes of Halobacterium halobium 总被引:9,自引:7,他引:2 下载免费PDF全文
In dried oriented samples of purple membrane isolated from Halobacterium halobium, the photoelectric activity decreases and the light adaptation vanishes when the water content of the sample is lowered. In the photocycle the first steps of the proton movement were accelerated with decreasing humidity, while the last steps of the photocycle could not be observed. From the analysis of the photoelectric signal we conclude that at low humidities the protons move forward in the L decay and return to their original place during M decay. 相似文献
17.
18.
19.
20.
Hana Votavová Vladimír Gut Karel Bláha Jaroslav Šponar 《International journal of biological macromolecules》1982,4(6):341-346
DNA complexes with polypeptides (Lys-Ala-Ala)1)] and (Lys-Ala-Ala)34 have been studied using the methods of thermal melting and circular dichroism. Derivative melting curves of (Lys-Ala-Ala)10 DNA differed substantially from those of (Lys-Ala-Ala)34 prepared either by salt gradient dialysis or by direct mixing. Melting curves of the former complex were unimodal or bimodal with Tm increasing continuously withn input lysin-to-DNA phosphate ratio (r); those of the latter complex consisted of three separate transitions with Tm values almost independent of r. Complete reversibility of binding in the (Lys-Ala-Ala)10-DNA system but a slow redistribution of (Lys-Ala-Ala)34 on DNA at low temperature were found in the redistribution experiments Much faster redistribution from denatured to native DNA occurs at the temperature of melting, contributing to the unusual trimodal melting pattern. Circular dichroism curves are very similar for both complexes and indicate little change of DNA conformation upon polypeptide binding. 相似文献