全文获取类型
收费全文 | 6127篇 |
免费 | 599篇 |
国内免费 | 3篇 |
出版年
2023年 | 39篇 |
2022年 | 91篇 |
2021年 | 215篇 |
2020年 | 105篇 |
2019年 | 158篇 |
2018年 | 196篇 |
2017年 | 150篇 |
2016年 | 235篇 |
2015年 | 417篇 |
2014年 | 365篇 |
2013年 | 396篇 |
2012年 | 540篇 |
2011年 | 502篇 |
2010年 | 307篇 |
2009年 | 213篇 |
2008年 | 343篇 |
2007年 | 349篇 |
2006年 | 313篇 |
2005年 | 281篇 |
2004年 | 256篇 |
2003年 | 237篇 |
2002年 | 214篇 |
2001年 | 55篇 |
2000年 | 46篇 |
1999年 | 69篇 |
1998年 | 56篇 |
1997年 | 31篇 |
1996年 | 21篇 |
1995年 | 21篇 |
1994年 | 22篇 |
1993年 | 21篇 |
1992年 | 36篇 |
1991年 | 29篇 |
1990年 | 21篇 |
1989年 | 16篇 |
1988年 | 28篇 |
1987年 | 30篇 |
1986年 | 30篇 |
1985年 | 29篇 |
1984年 | 12篇 |
1983年 | 23篇 |
1982年 | 13篇 |
1980年 | 16篇 |
1979年 | 15篇 |
1978年 | 21篇 |
1977年 | 14篇 |
1975年 | 13篇 |
1974年 | 13篇 |
1973年 | 13篇 |
1970年 | 12篇 |
排序方式: 共有6729条查询结果,搜索用时 46 毫秒
81.
Isolation and identification of the conidial germination factor of Neurospora crassa. 总被引:9,自引:5,他引:4 下载免费PDF全文
The germination-essential substance (germination factor [GF]) that is lost from conidia of Neurospora crassa on exposure to solutions of low water activity has been isolated and identified as a group of iron-transport compounds, or siderochromes. The principal siderochrome of conidia is ferricrocin, a cyclic hexapeptide. A closely related substance, ferrichrome C, is tentatively identified as a minor constituent. The same substances are also present in extracts of mycelium along with small amounts of a third siderochrome, which has not been identified. The GF activity of culture filtrates is due to coprogen, the only siderochrome previously identified with N. crassa. 相似文献
82.
Madritsch Silvia Bomers Svenja Posekany Alexandra Burg Agnes Birke Rebekka Emerstorfer Florian Turetschek Reinhard Otte Sandra Eigner Herbert Sehr Eva M. 《Plant molecular biology》2020,104(4-5):379-380
Plant Molecular Biology - In the above mentioned publication, part of Fig. 6B was distorted (extra diagonal lines appeared). The original article has been corrected and the proper version... 相似文献
83.
Alexandra A. Anderson Emma S. Child Aarathi Prasad Lucy M. Elphick David J. Mann 《Journal of cellular physiology》2010,225(3):638-645
D‐type cyclins predominantly regulate progression through the cell cycle by their interactions with cyclin‐dependent kinases (cdks). Here, we show that stimulating mitogenesis of Swiss 3T3 cells with phorbol esters or forskolin can induce divergent responses in the expression levels, localization and activation state of cyclin D1 and cyclin D3. Phorbol ester‐mediated protein kinase C stimulation induces S phase entry which is dependent on MAPK activation and increases the levels and activation of cyclin D1, whereas forskolin‐mediated cAMP‐dependent protein kinase A stimulation induces mitogenesis that is independent of MAPK, but dependent upon mTor and specifically increases the level and activation of cyclin D3. These findings uncover additional levels of complexity in the regulation of the cell cycle at the level of the D‐type cyclins and thus may have important therapeutic implications in cancers where specific D‐cyclins are overexpressed. J. Cell. Physiol. 225: 638–645, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
84.
Andreas Ritter Mourad Sanhaji Alexandra Friemel Susanne Roth Udo Rolle Frank Louwen Juping Yuan 《Cell cycle (Georgetown, Tex.)》2015,14(23):3755-3767
Mitotic centromere-associated kinesin (MCAK) is the best characterized member of the kinesin-13 family and plays important roles in microtubule dynamics during mitosis. Its activity and subcellular localization is tightly regulated by an orchestra of mitotic kinases, such as Aurora B. It is well known that serine 196 of MCAK is the major phosphorylation site of Aurora B in Xenopus leavis extracts and that this phosphorylation regulates its catalytic activity and subcellular localization. In the current study, we have addressed the conserved phosphorylation site serine 192 in human MCAK to characterize its function in more depth in human cancer cells. Our data confirm that S192 is the major phosphorylation site of Aurora B in human MCAK and that this phosphorylation has crucial roles in regulating its catalytic activity and localization at the kinetochore/centromere region in mitosis. Interfering with this phosphorylation leads to a delayed progression through prometa- and metaphase associated with mitotic defects in chromosome alignment and segregation. We show further that MCAK is involved in directional migration and invasion of tumor cells, and interestingly, interference with the S192 phosphorylation affects this capability of MCAK. These data provide the first molecular explanation for clinical observation, where an overexpression of MCAK was associated with lymphatic invasion and lymph node metastasis in gastric and colorectal cancer patients. 相似文献
85.
86.
E.R. Berman J. Horowitz N. Segal S. Fisher L. Feeney-Burns 《Biochimica et Biophysica Acta (BBA)/General Subjects》1980,630(1):36-46
The kinetic properties and subcellular distribution of an esterifying enzyme in the pigment epithelium of bovine retina have been studied using both [1-3H]retinol and [3H]retinol bound to cellular retinol-binding protein as substrates. The most active esterifying fraction in pigment epithelial cell preparations was the microsomes, but the lysosome plus mitochondria fraction also showed some activity, probably due to endoplasmic reticulum present as an impurity. The microsomal enzyme showed optimum activity at pH 7.5, and the reaction was linear up to 30 μg protein and for the first 10–15 min. The apparent Km values were 16.6 · 10?6 and 5.5 · 10?6 M for [3H]retinol and bound [3H]retinol, respectively. This is the first time that retinol bound to cellular retinol-binding protein has been shown to undergo metabolic stransformation. The microsomal esterifying activity was destroyed by boiling for 1 min, or after freezing for 2 months. No clear requirement for ATP, CoA or fatty acid could be demonstrated.Of all the other tissues examined under the same experimental conditions as those used for the pigment epithelium, onlt intestine showed measurable activity. With larger amounts of tissue protein and longer incubation periods, activity was also detectable in microsomes of liver, testis and retina 相似文献
87.
88.
89.
90.