全文获取类型
收费全文 | 22314篇 |
免费 | 2077篇 |
国内免费 | 13篇 |
专业分类
24404篇 |
出版年
2023年 | 123篇 |
2022年 | 315篇 |
2021年 | 588篇 |
2020年 | 272篇 |
2019年 | 392篇 |
2018年 | 450篇 |
2017年 | 355篇 |
2016年 | 615篇 |
2015年 | 1018篇 |
2014年 | 1063篇 |
2013年 | 1458篇 |
2012年 | 1676篇 |
2011年 | 1639篇 |
2010年 | 1018篇 |
2009年 | 882篇 |
2008年 | 1272篇 |
2007年 | 1262篇 |
2006年 | 1140篇 |
2005年 | 1078篇 |
2004年 | 967篇 |
2003年 | 912篇 |
2002年 | 867篇 |
2001年 | 254篇 |
2000年 | 208篇 |
1999年 | 227篇 |
1998年 | 194篇 |
1997年 | 161篇 |
1996年 | 130篇 |
1995年 | 121篇 |
1994年 | 128篇 |
1993年 | 134篇 |
1992年 | 159篇 |
1991年 | 137篇 |
1990年 | 139篇 |
1989年 | 125篇 |
1988年 | 115篇 |
1987年 | 122篇 |
1986年 | 121篇 |
1985年 | 140篇 |
1984年 | 150篇 |
1983年 | 127篇 |
1982年 | 130篇 |
1981年 | 125篇 |
1980年 | 122篇 |
1979年 | 114篇 |
1978年 | 83篇 |
1977年 | 86篇 |
1976年 | 88篇 |
1975年 | 91篇 |
1973年 | 82篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
91.
92.
Bacteria able to use at least one of 13 ionic alkylphosphonates of O-alkyl or O,O-dialkyl alkylphosphonates as phosphorus sources were isolated from sewage and soil. Four of these isolates used 2-aminoethylphosphonic acid (AEP) as a sole carbon, nitrogen, and phosphorus source. None of the other phosphonates served as a carbon source for the organisms. One isolate, identified as Pseudomonas putida, grew with AEP as its sole carbon, nitrogen, and phosphorus source and released nearly all of the organic phosphorus as orthophosphate and 72% of the AEP nitrogen as ammonium. This is the first demonstration of utilization of a phosphonoalkyl moiety as a sole carbon source. Cell-free extracts of P. putida contained an inducible enzyme system that required pyruvate and pyridoxal phosphate to release orthophosphate from AEP; acetaldehyde was tentatively identified as a second product. Phosphite inhibited the enzyme system. 相似文献
93.
94.
95.
R. McN. Alexander 《Journal of Zoology》1983,201(3):363-376
Pachyornis , which is extinct, was a huge bird with massively thick leg bones. These have been measured, to assess their strength. A mathematical model is used to calculate the forces that must have acted on them, when Pachyornis ran. Hence it is shown that the stresses likely to have acted on them are similar in magnitude to the stresses experienced (in stenuous activities) by bones of modem birds and mammals. The femur and tarsometatanus, however, seem disproportionately strong in comparison with the tibiotanus. 相似文献
96.
Abstract Some strains of motile Aeromonas produce lectin-like adhesins, whose activity can be inhibited by d -mannose. Such strains can co-agglutinate with some strains of Salmonella . Whether or not co-agglutination occurs is dependent upon both the properties of the Aeromonas adhesin and the structure of the Salmonella lipopolysaccharide (LPS). These studies have enabled new structural information for Salmonella LPS to be deduced and have confirmed previous studies regarding the nature of the Aeromonas adhesin binding site. It is possible that the observed in vitro co-agglutination between Aeromonas and Salmonella is a reflection of an in vivo situation which could modify the virulence of either or both bacteria. 相似文献
97.
Characterization of the calmodulin binding domain of neuromodulin. Functional significance of serine 41 and phenylalanine 42. 总被引:14,自引:0,他引:14
E R Chapman D Au K A Alexander T A Nicolson D R Storm 《The Journal of biological chemistry》1991,266(1):207-213
Neuromodulin (also designated P-57, GAP-43, B-50) is a major presynaptic substrate for protein kinase C. Phosphorylation of neuromodulin decreases its affinity for calmodulin, suggesting that neuromodulin may function to bind and concentrate calmodulin at specific sites within neurons, releasing calmodulin locally in response to phosphorylation by protein kinase C (Alexander, K. A., Cimler, B. M., Meier, K. E., and Storm, D. R. (1987) J. Biol. Chem. 262, 6108-6113). In the present study, we have constructed and characterized several mutant neuromodulins to demonstrate that the amino acid sequence 39-56 is required for calmodulin binding, and that this domain contains the sole in vitro protein kinase C phosphorylation site at serine 41. We also demonstrate that the adjacent phenylalanine 42, interacts hydrophobically with calmodulin. These hydrophobic interactions may be disrupted by the introduction of negative charge at serine 41, and thereby regulate the neuromodulin/calmodulin binding interactions. The sensitivity of the neuromodulin/calmodulin binding interaction to negative charge at serine 41 was determined by substitution of serine 41 with an aspartate or an asparagine residue. The asparagine mutant retained its affinity for calmodulin-Sepharose while the aspartate mutant did not adsorb to calmodulin-Sepharose. We conclude that protein kinase C phosphorylation of neuromodulin abolishes calmodulin binding by introducing negative charges within the calmodulin binding domain at a position adjacent to the phenylalanine. 相似文献
98.
Targeted disruption of the tissue inhibitor of metalloproteinases gene increases the invasive behavior of primitive mesenchymal cells derived from embryonic stem cells in vitro. 下载免费PDF全文
The metalloproteinase family of proteolytic enzymes can degrade extracellular matrix and facilitate invasive migration. This class of enzymes is specifically inhibited by the tissue inhibitor of metalloproteinases (TIMP-1). Using homologous recombination, we have disrupted the gene encoding TIMP-1 in pluripotent embryonic stem cells. Because the TIMP-1 gene is X linked and is hemizygous in embryonic stem cells, we have been able to study the effect of this mutation in culture. Using a basement membrane invasion assay, we found that the mutant cells, differentiated in low concentrations of serum with retinoic acid, were more invasive than their normal cell counterparts, and that this was specifically reversed by adding exogenous TIMP-1 protein. The invasive cell population had characteristics of an early population of primitive mesenchymal cells, including expression of vimentin and a transient period of invasiveness from 4-8 d after initiation of differentiation. Therefore, metalloproteinase activity can be rate limiting for cell invasion. 相似文献
99.
Characteristics of spontaneous embryonic loss in 21 mares were compared with those of 52 contemporary mares that maintained pregnancy. Embryonic losses were, in retrospect, grouped according to day of loss and length of the interovulatory interval, respectively, as follows: group 1, less than or equal to day 20 and less than or equal to 30 days (n = 10); group 2, less than or equal to day 20 and greater than 30 days (n = 3); and group 3, greater than day 20 and greater than 30 days (n = 8); ovulation was day 0. Mean diameter of the embryonic vesicle in group 1 was smaller (P less than 0.05) on days 12-18 than in the pregnancy-maintained group, but among the pregnancy-maintained group and the embryonic-loss groups, the mean individual growth rates of vesicles was similar (no significant difference). A more frequent (P less than 0.05) location of the vesicles in the uterine body on day 13 in group 1 was due to a greater proportion of small vesicles and for day 18 was due to a greater incidence of fixation failure. Luteal regression occurred at the expected time in 77% of the mares with loss sooner than day 20. Low concentration of progesterone on days 12, 15 and 18, a detected decrease in diameter of the corpus luteum on days 15 and 18, and an interovulatory interval of less than or equal to 30 days indicated that luteolysis was not prevented by the embryonic vesicle in group 1.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
100.
W K Samson B D Alexander K D Skala F L Huang R J Fulton 《Canadian journal of physiology and pharmacology》1992,70(5):773-778
A variety of neural factors can influence reproductive hormone secretion by neuromodulatory actions within the hypothalamus or neuroendocrine actions within the anterior pituitary gland. Passive immunoneutralization and antagonist administration protocols have suggested physiological roles for a number of these factors; however, both experimental approaches have severe technical limitations. We have developed novel methodology utilizing cytotoxin cell targeting with neuropeptides linked to the toxic A chain of the plant cytotoxin ricin. With this methodology we can target and destroy in vivo or in vitro cells bearing receptors for that peptide. Ricin A chain conjugated to atrial natriuretic peptide (ANP), a neuropeptide known to pharmacologically inhibit luteinizing hormone-releasing hormone (LHRH) release, was injected into the cerebroventricular system of intact, cycling rats and ovariectomized rats. Cytotoxin conjugate treatment significantly lengthened the estrous cycle. In ovariectomized rats the luteinizing hormone surge induced by steroid priming was completely inhibited. LHRH content of the median eminences of these rats was not significantly altered. These data suggest that ANP binding to clearance receptors in the hypothalamus displaces the C-type natriuretic peptide (CNP) from the shared clearance receptor, making more CNP available to inhibit LHRH release. In the absence of cells bearing the clearance receptor all available CNP binds to the ANPR-B receptor and exerts its effect via an inhibitory interneuron, since LHRH fibers are spared by this treatment. 相似文献