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971.
High salinity is an environmental factor that inhibits plant growth and development, leading to large losses in crop yields.
We report here that mutations in SIZ1 or PHO2, which cause more accumulation of phosphate compared with the wild type, enhance tolerance to salt stress. The siz1 and pho2 mutations reduce the uptake and accumulation of Na+. These mutations are also able to suppress the Na+ hypersensitivity of the sos3-1 mutant, and genetic analyses suggest that SIZ1 and SOS3 or PHO2 and SOS3 have an additive effect on the response to salt stress. Furthermore, the siz1 mutation cannot suppress the Li+ hypersensitivity of the sos3-1 mutant. These results indicate that the phosphate-accumulating mutants siz1 and pho2 reduce the uptake and accumulation of Na+, leading to enhanced salt tolerance, and that, genetically, SIZ1 and PHO2 are likely independent of SOS3-dependent salt signaling. 相似文献
972.
We have investigated the local and regional scale genetic structure of Siberian primrose (Primula nutans) populations in Northern Europe. The genetic diversity and structure of fifteen populations sampled from the Bothnian Bay
in Finland, the Barents Sea in Norway and the White Sea in Russia were assessed using eleven microsatellite markers. We investigated
the distribution of genetic variation within and between populations, and studied the local genetic structure using spatial
autocorrelation analysis. We found very low genetic and allelic diversity in the Bothnian Bay and Barents Sea populations,
and only slightly higher in the White Sea population. The level of genetic differentiation between the regions was very high,
whereas differentiation between the populations within the regions was moderate. We found no spatial structuring of populations
in any region suggesting efficient dispersal on a local scale. Clonal reproduction seemed to have no effect on genetic structure. 相似文献
973.
Nino Asatiani Tamar Kartvelishvili Marina Abuladze Lali Asanishvili Nelly Sapojnikova 《Biological trace element research》2011,142(3):388-397
The changes in glutathione-dependent cycle enzymes and catalase activities under Cr(VI)-induced oxidative stress were investigated
in two distinct cell lines: L-41−human epithelial-like cells and HLF−fetal human diploid lung fibroblasts, which differ in
tissue origin, proliferation, and antioxidant enzymes activities. The chromium concentrations from 1 to 5 μM cause nontoxic
effects and activate antioxidant enzymes to overcome oxidative stress. In spite of some differences in the endogenous antioxidant
activities, both cell lines reveal the same range of toxic concentrations (20–30 μM). The irreversible inhibition of glutathione-dependent
antioxidant enzymes develops under toxic concentrations and serves as a marker of toxicity. The endogenous antioxidant activity
influences time-dependent expression of Cr(VI) toxicity and the dynamics of antioxidant enzymes activity under nontoxic conditions.
The cell antioxidant defense system is an important marker of the cell adaptive capacity under nontoxic and toxic conditions. 相似文献
974.
Park SJ Park BJ Jung MY Kim SJ Chae JC Roh Y Forwick M Yoon HI Rhee SK 《Microbial ecology》2011,62(3):537-548
Increases in global temperatures have been shown to enhance glacier melting in the Arctic region. Here, we have evaluated
the effects of meltwater runoff on the microbial communities of coastal marine sediment located along a transect of Temelfjorden,
in Svalbard. As close to the glacier front, the sediment properties were clearly influenced by deglaciation. Denaturing gradient
gel electrophoresis profiles showed that the sediment microbial communities of the stations of glacier front (stations 188–178)
were distinguishable from that of outer fjord region (station 176). Canonical correspondence analysis indicated that total
carbon and calcium carbonate in sediment and chlorophyll a in bottom water were key factors driving the change of microbial
communities. Analysis of 16S rRNA gene clone libraries suggested that microbial diversity was higher within the glacier–proximal
zone (station 188) directly affected by the runoffs than in the outer fjord region. While the crenarchaeotal group I.1a dominated
at station 176 (62%), Marine Benthic Group-B and other Crenarchaeota groups were proportionally abundant. With regard to the
bacterial community, alpha-Proteobacteria and Flavobacteria lineages prevailed (60%) at station 188, whereas delta-Proteobacteria (largely sulfate-reducers) predominated (32%) at station 176. Considering no clone sequences related to sulfate-reducers,
station 188 may be more oxic compared to station 176. The distance-wise compositional variation in the microbial communities
is attributable to their adaptations to the sediment environments which are differentially affected by melting glaciers. 相似文献
975.
976.
Guillaume Gayet Cyril Eraud Maurice Benmergui Joël Broyer François Mesleard Hervé Fritz Matthieu Guillemain 《European Journal of Wildlife Research》2011,57(5):1051-1056
A number of native and exotic animal species show dramatic population increases in terms of both numbers and geographic range.
Understanding the habitat selection processes behind such increases is crucial to implement adequate management measures.
Mute swan (Cygnus olor) populations have experienced a tremendous demographic and geographic expansion in Western Europe during the twentieth century,
colonizing a wide variety of aquatic habitats. We aimed at assessing how swans select nesting sites during the pre-laying
and laying periods on medium to large fishponds (from 10 to 50 ha) in Eastern France, while accounting for detectability biases
and testing for the effects of fishpond spatial configuration, vegetation resources, human disturbance and habitat management.
Our results demonstrate that the mute swan is a non-selective species regarding its nesting habitat among such fishponds,
using these independently from the parameters considered although fishpond characteristics varied. Although mute swan is one
of the least cryptic Anatidae, owing to its white colour and large size, detection of breeding pairs remained imperfect for
each over several sampling occasions. However, because we repeated the sampling sessions, detection of swan pairs by the end
of the monitoring period was as high as 0.94. These results are consistent with previous assertions that the mute swan is
a species of high ecological plasticity, which may partly explain its recent colonization rates. Given that even swan breeding
events were imperfectly detected on each occasion, we highlight the fact that most studies of breeding ducks (which are more
cryptic) would be considerably improved by better considering detection biases. 相似文献
977.
Mai Kanke Kohei Nishimura Masato Kanemaki Tatsuo Kakimoto Tatsuro S Takahashi Takuro Nakagawa Hisao Masukata 《BMC cell biology》2011,12(1):8
Background
Inducible inactivation of a protein is a powerful approach for analysis of its function within cells. Fission yeast is a useful model for studying the fundamental mechanisms such as chromosome maintenance and cell cycle. However, previously published strategies for protein-depletion are successful only for some proteins in some specific conditions and still do not achieve efficient depletion to cause acute phenotypes such as immediate cell cycle arrest. The aim of this work was to construct a useful and powerful protein-depletion system in Shizosaccaromyces pombe. 相似文献978.
Matthew J Christmas Julia C Jones Anna Olsson Ola Wallerman Ignas Bunikis Marcin Kierczak Valentina Peona Kaitlyn M Whitley Tuuli Larva Alexander Suh Nicole E Miller-Struttmann Jennifer C Geib Matthew T Webster 《Molecular biology and evolution》2021,38(8):3126
Evidence is accumulating that gene flow commonly occurs between recently diverged species, despite the existence of barriers to gene flow in their genomes. However, we still know little about what regions of the genome become barriers to gene flow and how such barriers form. Here, we compare genetic differentiation across the genomes of bumblebee species living in sympatry and allopatry to reveal the potential impact of gene flow during species divergence and uncover genetic barrier loci. We first compared the genomes of the alpine bumblebee Bombus sylvicola and a previously unidentified sister species living in sympatry in the Rocky Mountains, revealing prominent islands of elevated genetic divergence in the genome that colocalize with centromeres and regions of low recombination. This same pattern is observed between the genomes of another pair of closely related species living in allopatry (B. bifarius and B. vancouverensis). Strikingly however, the genomic islands exhibit significantly elevated absolute divergence (dXY) in the sympatric, but not the allopatric, comparison indicating that they contain loci that have acted as barriers to historical gene flow in sympatry. Our results suggest that intrinsic barriers to gene flow between species may often accumulate in regions of low recombination and near centromeres through processes such as genetic hitchhiking, and that divergence in these regions is accentuated in the presence of gene flow. 相似文献
979.
Qayyum R Snively BM Ziv E Nalls MA Liu Y Tang W Yanek LR Lange L Evans MK Ganesh S Austin MA Lettre G Becker DM Zonderman AB Singleton AB Harris TB Mohler ER Logsdon BA Kooperberg C Folsom AR Wilson JG Becker LC Reiner AP 《PLoS genetics》2012,8(3):e1002491
Several genetic variants associated with platelet count and mean platelet volume (MPV) were recently reported in people of European ancestry. In this meta-analysis of 7 genome-wide association studies (GWAS) enrolling African Americans, our aim was to identify novel genetic variants associated with platelet count and MPV. For all cohorts, GWAS analysis was performed using additive models after adjusting for age, sex, and population stratification. For both platelet phenotypes, meta-analyses were conducted using inverse-variance weighted fixed-effect models. Platelet aggregation assays in whole blood were performed in the participants of the GeneSTAR cohort. Genetic variants in ten independent regions were associated with platelet count (N?=?16,388) with p<5×10(-8) of which 5 have not been associated with platelet count in previous GWAS. The novel genetic variants associated with platelet count were in the following regions (the most significant SNP, closest gene, and p-value): 6p22 (rs12526480, LRRC16A, p?=?9.1×10(-9)), 7q11 (rs13236689, CD36, p?=?2.8×10(-9)), 10q21 (rs7896518, JMJD1C, p?=?2.3×10(-12)), 11q13 (rs477895, BAD, p?=?4.9×10(-8)), and 20q13 (rs151361, SLMO2, p?=?9.4×10(-9)). Three of these loci (10q21, 11q13, and 20q13) were replicated in European Americans (N?=?14,909) and one (11q13) in Hispanic Americans (N?=?3,462). For MPV (N?=?4,531), genetic variants in 3 regions were significant at p<5×10(-8), two of which were also associated with platelet count. Previously reported regions that were also significant in this study were 6p21, 6q23, 7q22, 12q24, and 19p13 for platelet count and 7q22, 17q11, and 19p13 for MPV. The most significant SNP in 1 region was also associated with ADP-induced maximal platelet aggregation in whole blood (12q24). Thus through a meta-analysis of GWAS enrolling African Americans, we have identified 5 novel regions associated with platelet count of which 3 were replicated in other ethnic groups. In addition, we also found one region associated with platelet aggregation that may play a potential role in atherothrombosis. 相似文献
980.
Chen H Hayashi G Lai OY Dilthey A Kuebler PJ Wong TV Martin MP Fernandez Vina MA McVean G Wabl M Leslie KS Maurer T Martin JN Deeks SG Carrington M Bowcock AM Nixon DF Liao W 《PLoS genetics》2012,8(2):e1002514
An important paradigm in evolutionary genetics is that of a delicate balance between genetic variants that favorably boost host control of infection but which may unfavorably increase susceptibility to autoimmune disease. Here, we investigated whether patients with psoriasis, a common immune-mediated disease of the skin, are enriched for genetic variants that limit the ability of HIV-1 virus to replicate after infection. We analyzed the HLA class I and class II alleles of 1,727 Caucasian psoriasis cases and 3,581 controls and found that psoriasis patients are significantly more likely than controls to have gene variants that are protective against HIV-1 disease. This includes several HLA class I alleles associated with HIV-1 control; amino acid residues at HLA-B positions 67, 70, and 97 that mediate HIV-1 peptide binding; and the deletion polymorphism rs67384697 associated with high surface expression of HLA-C. We also found that the compound genotype KIR3DS1 plus HLA-B Bw4-80I, which respectively encode a natural killer cell activating receptor and its putative ligand, significantly increased psoriasis susceptibility. This compound genotype has also been associated with delay of progression to AIDS. Together, our results suggest that genetic variants that contribute to anti-viral immunity may predispose to the development of psoriasis. 相似文献