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91.
Peggy CR Godschalk Mathijs P Bergman Raymond FJ Gorkink Guus Simons Nicole van den Braak Albert J Lastovica Hubert P Endtz Henri A Verbrugh Alex van Belkum 《BMC microbiology》2006,6(1):32-13
Background
Campylobacter jejuni is the predominant cause of antecedent infection in post-infectious neuropathies such as the Guillain-Barré (GBS) and Miller Fisher syndromes (MFS). GBS and MFS are probably induced by molecular mimicry between human gangliosides and bacterial lipo-oligosaccharides (LOS). This study describes a new C. jejuni-specific high-throughput AFLP (htAFLP) approach for detection and identification of DNA polymorphism, in general, and of putative GBS/MFS-markers, in particular. 相似文献92.
Omenn GS States DJ Adamski M Blackwell TW Menon R Hermjakob H Apweiler R Haab BB Simpson RJ Eddes JS Kapp EA Moritz RL Chan DW Rai AJ Admon A Aebersold R Eng J Hancock WS Hefta SA Meyer H Paik YK Yoo JS Ping P Pounds J Adkins J Qian X Wang R Wasinger V Wu CY Zhao X Zeng R Archakov A Tsugita A Beer I Pandey A Pisano M Andrews P Tammen H Speicher DW Hanash SM 《Proteomics》2005,5(13):3226-3245
HUPO initiated the Plasma Proteome Project (PPP) in 2002. Its pilot phase has (1) evaluated advantages and limitations of many depletion, fractionation, and MS technology platforms; (2) compared PPP reference specimens of human serum and EDTA, heparin, and citrate-anti-coagulated plasma; and (3) created a publicly-available knowledge base (www.bioinformatics.med.umich.edu/hupo/ppp; www.ebi.ac.uk/pride). Thirty-five participating laboratories in 13 countries submitted datasets. Working groups addressed (a) specimen stability and protein concentrations; (b) protein identifications from 18 MS/MS datasets; (c) independent analyses from raw MS-MS spectra; (d) search engine performance, subproteome analyses, and biological insights; (e) antibody arrays; and (f) direct MS/SELDI analyses. MS-MS datasets had 15 710 different International Protein Index (IPI) protein IDs; our integration algorithm applied to multiple matches of peptide sequences yielded 9504 IPI proteins identified with one or more peptides and 3020 proteins identified with two or more peptides (the Core Dataset). These proteins have been characterized with Gene Ontology, InterPro, Novartis Atlas, OMIM, and immunoassay-based concentration determinations. The database permits examination of many other subsets, such as 1274 proteins identified with three or more peptides. Reverse protein to DNA matching identified proteins for 118 previously unidentified ORFs. We recommend use of plasma instead of serum, with EDTA (or citrate) for anticoagulation. To improve resolution, sensitivity and reproducibility of peptide identifications and protein matches, we recommend combinations of depletion, fractionation, and MS/MS technologies, with explicit criteria for evaluation of spectra, use of search algorithms, and integration of homologous protein matches. This Special Issue of PROTEOMICS presents papers integral to the collaborative analysis plus many reports of supplementary work on various aspects of the PPP workplan. These PPP results on complexity, dynamic range, incomplete sampling, false-positive matches, and integration of diverse datasets for plasma and serum proteins lay a foundation for development and validation of circulating protein biomarkers in health and disease. 相似文献
93.
94.
Alex Paton 《BMJ (Clinical research ed.)》1983,286(6370):1030-1031
95.
Summary The authors show that in certain isolated tissues (cornea of frog, mouse cancer) the mitotic processes continue during at least one hour. They are very strongly stimulated by heat and also by mitogenetic radiation. In the case of the cancer cells new mitoses are promoted in considerable number. A detailed analysis of both energy factors leads to the conclusion that they effect a disturbance of the unstable constellations (structures) of the elementary particles in the cell-body. Thus a certain degree of disorganisation of its plasma seems to stimulate cell-division. This statement is, in the opinion of the authors a proof that cell-division is controlled by a field, the trajectories of the elementary particles of the cell-body being a function of their coordinates relative to the cell-axes.
Résumé Les auteurs démontrent que dans certains tissus survivants (la cornée des yeux de grenouille et le cancer de souris) les mitoses continuent leur processus d'évolution durant au moins une heure. Par l'application de chaleur aussi bien que par l'irradiation mitogénétique on peut accélérer beaucoup le rhythme des mitoses en cours d'évolution et provoquer (dans les cas de cancer seulement) l'apparition d'un nombre considérable de nouvelles mitoses. L'analyse de l'action de ces deux facteurs d'énergie aboutit à la conclusion qu'il ne peut s'agir que d'un dérangement des constellations instables des éléments du corps cellulaire. On peut en déduire qu'un certain degré de désorganisation du plasme est favorable aux divisions cellulaires. En poursuivant et en développant cette conception, les auteurs arrivent à la conception du champ cellulaire de division, définissant les trajectoires des particules élémentaires comme fonction de ses coordonnés relativement aux axes des cellules.相似文献
96.
A trypsin and chymotrypsin inhibitor was partially purified from Bauhenia purpurea seeds and separated from a second inhibitor by Ecteola cellulose chromatography. The factor inhibited bovine trypsin and chymotrypsin as well as pronase trypsin and elastase. It formed a complex with trypsin and with chymotrypsin, but a ternary complex could not be detected. Differences were detected in the effect on trypsin and on chymotrypsin, although one enzyme interfered with the inhibition of the other. The results obtained point to two active centers on the inhibitor for the trypsin and chymotrypsin inhibition such that the one cannot complex with the inhibitor after this inhibitor had complexed with the other. 相似文献
98.
Circumcision is often claimed to be simpler, safer and more cost-effective when performed in the neonatal period as opposed to later in life, with a greater benefit-to-risk ratio. In the first part of this paper, we critically examine the evidence base for these claims, and find that it is not as robust as is commonly assumed. In the second part, we demonstrate that, even if one simply grants these claims for the sake of argument, it still does not follow that neonatal circumcision is ethically permissible absent urgent medical necessity. Based on a careful consideration of the relevant evidence, arguments and counterarguments, we conclude that medically unnecessary penile circumcision—like other medically unnecessary genital procedures, such as ‘cosmetic’ labiaplasty—should not be performed on individuals who are too young (or otherwise unable) to provide meaningful consent to the procedure. 相似文献
99.
Consumption of foods that are high in fat contribute to obesity and metabolism‐related disorders. Dietary lipids are comprised of triglycerides and fatty acids, and the highly palatable taste of dietary fatty acids promotes food consumption, activates reward centers in mammals and underlies hedonic feeding. Despite the central role of dietary fats in the regulation of food intake and the etiology of metabolic diseases, little is known about how fat consumption regulates sleep. The fruit fly, Drosophila melanogaster, provides a powerful model system for the study of sleep and metabolic traits, and flies potently regulate sleep in accordance with food availability. To investigate the effects of dietary fats on sleep regulation, we have supplemented fatty acids into the diet of Drosophila and measured their effects on sleep and activity. We found that flies fed a diet of hexanoic acid, a medium‐chain fatty acid that is a by‐product of yeast fermentation, slept more than flies starved on an agar diet. To assess whether dietary fatty acids regulate sleep through the taste system, we assessed sleep in flies with a mutation in the hexanoic acid receptor Ionotropic receptor 56D, which is required for fatty acid taste perception. We found that these flies also sleep more than agar‐fed flies when fed a hexanoic acid diet, suggesting the sleep promoting effect of hexanoic acid is not dependent on sensory perception. Taken together, these findings provide a platform to investigate the molecular and neural basis for fatty acid‐dependent modulation of sleep. 相似文献