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排序方式: 共有1100条查询结果,搜索用时 203 毫秒
991.
Sgrelli A Mencacci A Fiorio M Orlandi C Baldelli F De Socio GV 《The new microbiologica》2012,35(2):245-247
We describe a case of a 66-year-old immunocompetent man affected by Achromobacter denitrificans renal abscess related to renal stones. The patient was treated successfully with meropenem 1 g three times daily for 60 days. To our knowledge, this is the first ever case reported of Achromobacter denitrificans renal abscess. 相似文献
992.
M Bonomi E Somigliana C Cacciatore M Busnelli R Rossetti S Bonetti A Paffoni D Mari G Ragni L Persani;the Italian Network for the study of Ovarian Dysfunctions 《PloS one》2012,7(8):e42423
Primary ovarian insufficiency (POI) is a critical fertility defect characterized by an anticipated and silent impairment of the follicular reserve, but its pathogenesis is largely unexplained. The frequent maternal inheritance of POI together with a remarkable dependence of ovarian folliculogenesis upon mitochondrial biogenesis and bioenergetics suggested the possible involvement of a generalized mitochondrial defect. Here, we verified the existence of a significant correlation between blood and ovarian mitochondrial DNA (mtDNA) content in a group of women undergoing ovarian hyperstimulation (OH), and then aimed to verify whether mtDNA content was significantly altered in the blood cells of POI women. We recruited 101 women with an impaired ovarian reserve: 59 women with premature ovarian failure (POF) and 42 poor responders (PR) to OH. A Taqman copy number assay revealed a significant mtDNA depletion (P<0.001) in both POF and PR women in comparison with 43 women of similar age and intact ovarian reserve, or 53 very old women with a previous physiological menopause. No pathogenic variations in the mitochondrial DNA polymerase γ (POLG) gene were detected in 57 POF or PR women with low blood mtDNA content. In conclusion, blood cell mtDNA depletion is a frequent finding among women with premature ovarian aging, suggesting that a still undetermined but generalized mitochondrial defect may frequently predispose to POI which could then be considered a form of anticipated aging in which the ovarian defect may represent the first manifestation. The determination of mtDNA content in blood may become an useful tool for the POI risk prediction. 相似文献
993.
Marverti G Guaitoli G Ligabue A Frassineti C Monti MG Lombardi P Costi MP 《Amino acids》2012,42(2-3):641-653
Acquired resistance to cisplatin (cDDP) is a multifactorial process that represents one of the main problems in ovarian cancer therapy. Distamycin A is a minor groove DNA binder whose toxicity has limited its use and prompted the synthesis of derivatives such as NAX001 and NAX002, which have a carbamoyl moiety and different numbers of pyrrolamidine groups. Their interaction with a B-DNA model and with an extended-TATA box model, [Polyd(AT)], was investigated using isothermal titration calorimetry (ITC) to better understand their mechanism of interaction with DNA and therefore better explain their cellular effects. Distamycin A interactions with Dickerson and Poly[d(AT)(6)] oligonucleotides show a different thermodynamic with respect to NAX002. The bulkier distamycin A analogue shows a non optimal binding to DNA due to its additional pyrrolamidine group. Cellular assays performed on cDDP-sensitive and -resistant cells showed that these compounds, distamycin A in particular, affect the expression of folate cycle enzymes even at cellular level. The optimal interaction of distamycin A with DNA may account for the down-regulation of both dihydrofolate reductase (DHFR) and thymidylate synthase (TS) and the up-regulation of spermidine/spermine N1-acetyltransferase (SSAT) caused by this compound. These effects seem differently modulated by the cDDP-resistance phenotype. NAX002 which presents a lower affinity to DNA and slightly affected these enzymes, showed a synergic inhibition profile in combination with cDDP. In addition, their combination with cDDP or polyamine analogues increased cell sensitivity to the drugs suggesting that these interactions may have potential for development in the treatment of ovarian carcinoma. 相似文献
994.
Lang M Sazzini M Calabrese FM Simone D Boattini A Romeo G Luiselli D Attimonelli M Gasparre G 《Human genetics》2012,131(5):757-771
The human genome is constantly subjected to evolutionary forces which shape its architecture. Insertions of mitochondrial
DNA sequences into nuclear genome (NumtS) have been described in several eukaryotic species, including Homo sapiens and other primates. The ongoing process of the generation of NumtS has made them valuable markers in primate phylogenetic
studies, as well as potentially informative loci for reconstructing the genetic history of modern humans. Here, we report
the identification of 53 human-specific NumtS by inspection of the UCSC genome browser, showing that they may be direct insertions
of mitochondrial DNA into the human nuclear DNA after the human-chimpanzee split. In silico analyses allowed us to identify
14 NumtS which are polymorphic in terms of their presence/absence within the human genome in individuals of different ancestry.
The allele frequencies of these polymorphic NumtS were calculated for 1000 Genomes Project sequence data from 13 populations
worldwide, and principal components analysis and hierarchical clustering methods allowed the detection of strong signals of
geographical structure related to the genetic diversity of these loci. All identified polymorphic human-specific NumtS together
with a tandemly duplicated NumtS have also been validated by PCR amplification on a panel of 60 samples belonging to five
native populations worldwide, confirming the expected NumtS variability. On the basis of these findings, we have succeeded
in depicting the landscape of variation of a series of NumtS in several ethnic groups, making an advance in their identification
as useful markers in the study on human population genetics. 相似文献
995.
996.
Inga Bauer Alessia Grozio Denise Lasigliè Giovanna Basile Laura Sturla Mirko Magnone Giovanna Sociali Debora Soncini Irene Caffa Alessandro Poggi Gabriele Zoppoli Michele Cea Georg Feldmann Raul Mostoslavsky Alberto Ballestrero Franco Patrone Santina Bruzzone Alessio Nencioni 《The Journal of biological chemistry》2012,287(49):40924-40937
997.
998.
Verderio C Cagnoli C Bergami M Francolini M Schenk U Colombo A Riganti L Frassoni C Zuccaro E Danglot L Wilhelm C Galli T Canossa M Matteoli M 《Biology of the cell / under the auspices of the European Cell Biology Organization》2012,104(4):213-228
Background information
ATP is the main transmitter stored and released from astrocytes under physiological and pathological conditions. Morphological and functional evidence suggest that besides secretory granules, secretory lysosomes release ATP. However, the molecular mechanisms involved in astrocytic lysosome fusion remain still unknown.Results
In the present study, we identify tetanus neurotoxin‐insensitive vesicle‐associated membrane protein (TI‐VAMP, also called VAMP7) as the vesicular SNARE which mediates secretory lysosome exocytosis, contributing to release of both ATP and cathepsin B from glial cells. We also demonstrate that fusion of secretory lysosomes is triggered by slow and locally restricted calcium elevations, distinct from calcium spikes which induce the fusion of glutamate‐containing clear vesicles. Downregulation of TI‐VAMP/VAMP7 expression inhibited the fusion of ATP‐storing vesicles and ATP‐mediated calcium wave propagation. TI‐VAMP/VAMP7 downregulation also significantly reduced secretion of cathepsin B from glioma.Conclusions
Given that sustained ATP release from glia upon injury greatly contributes to secondary brain damage and cathepsin B plays a critical role in glioma dissemination, TI‐VAMP silencing can represent a novel strategy to control lysosome fusion in pathological conditions. 相似文献999.
Bennett CE Burnett DA Greenlee WJ Knutson CE Korakas P Li C Tulshian D Wu WL Bertorelli R Fredduzzi S Grilli M Lozza G Reggiani A Veltri A 《Bioorganic & medicinal chemistry letters》2012,22(4):1575-1578
A series of fused tricyclic mGluR1 antagonists containing a pyridone ring were synthesized. In vitro, these antagonists were potent against both human and rat isozymes, as well as selective for inhibiting mGluR1 over mGluR5. When dosed orally, several examples were active in vivo in a rat SNL test. 相似文献
1000.