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71.
Deyze Alencar Soares Marilia Barros Oliveira Adriane Feijó Evangelista Emerson José Venancio Rosangela Vieira Andrade Maria Sueli Soares Felipe Silvana Petrofeza 《Memórias do Instituto Oswaldo Cruz》2013,108(6):808-811
Phospholipase is an important virulence factor for pathogenic fungi. In this
study, we demonstrate the following: (i) the Paracoccidioides
brasiliensis pld gene is preferentially expressed in mycelium
cells, (ii) the plb1 gene is mostly up-regulated by infection
after 6 h of co-infection of MH-S cells or during BALB/c mice
lung infection, (iii) during lung infection, plb1,
plc and pld gene expression are
significantly increased 6-48 h post-infection compared to 56 days after
infection, strongly suggesting that phospholipases play a role in the early
events of infection, but not during the chronic stages of pulmonary infection by
P. brasiliensis. 相似文献
72.
Ramiro M. Murata Luciana S. Branco-de-Almeida Eliane M. Franco Regiane Yatsuda Marcelo H. dos Santos Severino M. de Alencar 《Biofouling》2013,29(7):865-872
7-Epiclusianone (7-epi), a novel naturally occurring compound isolated from Rheedia brasiliensis, effectively inhibits the synthesis of exopolymers and biofilm formation by Streptococcus mutans. In the present study, the ability of 7-epi, alone or in combination with fluoride (F), to disrupt biofilm development and pathogenicity of S. mutans in vivo was examined using a rodent model of dental caries. Treatment (twice-daily, 60s exposure) with 7-epi, alone or in combination with 125 ppm F, resulted in biofilms with less biomass and fewer insoluble glucans than did those treated with vehicle-control, and they also displayed significant cariostatic effects in vivo (p < 0.05). The combination 7-epi + 125 ppm F was as effective as 250 ppm F (positive-control) in reducing the development of both smooth- and sulcal-caries. No histopathological alterations were observed in the animals after the experimental period. The data show that 7-epiclusianone is a novel and effective antibiofilm/anticaries agent, which may enhance the cariostatic properties of fluoride. 相似文献
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75.
Oliveira Daelen Resende Lopes Ana Claudia Alencar Pereira Rafaela Andrade Cardoso Patricia Gomes Duarte Whasley Ferreira 《Annals of microbiology》2019,69(13):1361-1372
This work aimed to assess the probiotic potential of different Kluyveromyces lactis strains isolated from Canastra cheese and to produce a fermented cheese whey beverage added to beetroot juice using the selected strain. Kluyveromyces lactis strains were tested for their resistance to the passage through the simulated gastrointestinal tract, adhesion properties, and functional effects such as inhibition of enteric pathogens, short-chain fatty acids (SCFA) production, and β-galactosidase activity. The selected strain was used to produce a fermented cheese whey beverage added to beetroot juice in different proportions. The produced beverages were characterized using HPLC for sugars, Folin-Ciocalteu for total phenolic content, DPPH for antioxidant activity, and GC-MS for volatiles compounds. Except B51, all strains showed viability above 75% after exposure to the simulated gastric and duodenal juices. The aggregation rates were above 84% in 24 h. Only B9 and C16 strains presented hydrophobicity above 60%. The highest B9 β-galactosidase activities were 2.17 U/g and 2.21 U/g for pH 7 and 9, respectively. The B9 SCFA profile was similar to that found for Saccharomyces bourllardi. The fermented cheese whey beverages presented phenolic content ranging from 102.75 to 291.61 μg EAG/mL and inhibition of DPPH ranging from 38.69 to 81.02% after 21 days of storage, besides being lactose free. Esters and acetates were the most abundant compounds. Kluyveromyces lactis B9 presented interesting results as a potential probiotic yeast. The produced beverages allowed the delivery of K. lactis B9 through innovative product with functional properties. 相似文献
76.
Maria José Couto Oliveira Fernando do Couto Motta Marilda M Siqueira Paola Cristina Resende Priscilla da Silva Born Thiago Moreno L Souza Milene Mesquita Maria de Lourdes Aguiar Oliveira Sharon Carney Wyller Alencar de Mello Vera Magalh?es 《Memórias do Instituto Oswaldo Cruz》2014,109(7):912-917
After the World Health Organization officially declared the end of the first pandemic
of the XXI century in August 2010, the influenza A(H1N1)pdm09 virus has been
disseminated in the human population. In spite of its sustained circulation, very
little on phylogenetic data or oseltamivir (OST) resistance is available for the
virus in equatorial regions of South America. In order to shed more light on this
topic, we analysed the haemagglutinin (HA) and neuraminidase (NA) genes of influenza
A(H1N1)pdm09 positive samples collected during the pandemic period in the Pernambuco
(PE), a northeastern Brazilian state. Complete HA sequences were compared and amino
acid changes were related to clinical outcome. In addition, the H275Y substitution in
NA, associated with OST resistance, was investigated by pyrosequencing. Samples from
PE were grouped in phylogenetic clades 6 and 7, being clustered together with
sequences from South and Southeast Brazil. The D222N/G HA gene mutation, associated
with severity, was found in one deceased patient that was pregnant. Additionally, the
HA mutation K308E, which appeared in Brazil in 2010 and was only detected worldwide
the following year, was identified in samples from hospitalised cases. The resistance
marker H275Y was not identified in samples tested. However, broader studies are
needed to establish the real frequency of resistance in this Brazilian region. 相似文献
77.
Isabella Coimbra Magda Maruza Maria de Fátima Pessoa Milit?o Albuquerque Joanna D’Arc Lyra Batista Maria Cynthia Braga Líbia Vilela Moura Demócrito Barros Miranda-Filho Ulisses Ramos Montarroyos Heloísa Ramos Lacerda Laura Cunha Rodrigues Ricardo Arraes de Alencar Ximenes 《PloS one》2014,9(4)
Background
The challenge of diagnosing smear-negative pulmonary TB (tuberculosis) in people living with HIV justifies the use of instruments other than the smear test for diagnosing the disease. Considering the clinical-radiological similarities of TB amongst HIV-infected adults and children, the proposal of this study was to assess the accuracy of a scoring system used to diagnose smear-negative pulmonary TB in children and adolescents, in HIV-infected adults suspected of having smear-negative pulmonary TB.Methods
A Phase III validation study aiming to assess the diagnostic accuracy of a scoring system for diagnosing smear-negative pulmonary TB in HIV-infected adults. The study assessed sensitivity, specificity, positive and negative likelihood ratios, and positive and negative predictive values of the scoring system. Three versions of the scoring system were tested.Results
From a cohort of 2,382 (HIV-infected adults), 1276 were investigated and 128 were diagnosed with pulmonary TB. Variables associated with the diagnosis of TB were: coughing, weight loss, fever, malnutrition, chest X-ray, and positive tuberculin test. The best diagnostic performance occurred with the scoring system with new scores, with sensitivity = 81.2% (95%-CI 74.5% –88%), specificity = 78% (75.6% –80.4%), PPV = 29.2% (24.5% –33.9%) and NPV = 97.4% (96.4% –98.4%), LR+ = 3.7 (3.4–4.0) and LR− = 0.24 (0.2–0.4).Conclusion
The proposed scoring system (with new scores) presented a good capacity for discriminating patients who did not have pulmonary TB, in the studied population. Further studies are necessary in order to validate it, thus permitting the assessment of its use in diagnosing smear-negative pulmonary TB in HIV-infected adults. 相似文献78.
Ricardo Arraes de Alencar Ximenes Celina Maria Turchi Martelli Marcos Amaku Ana Marli C. Sartori Patricia Coelho de Soárez Hillegonda Maria Dutilh Novaes Leila Maria Moreira Beltr?o Pereira Regina Célia Moreira Gerusa Maria Figueiredo Raymundo Soares de Azevedo for the Hepatitis Study Group 《PloS one》2014,9(5)
Background
This study aimed to identify the transmission pattern of hepatitis A (HA) infection based on a primary dataset from the Brazilian National Hepatitis Survey in a pre-vaccination context. The national survey conducted in urban areas disclosed two epidemiological scenarios with low and intermediate HA endemicity.Methods
A catalytic model of HA transmission was built based on a national seroprevalence survey (2005 to 2009). The seroprevalence data from 7,062 individuals aged 5–69 years from all the Brazilian macro-regions were included. We built up three models: fully homogeneous mixing model, with constant contact pattern; the highly assortative model and the highly assortative model with the additional component accounting for contacts with infected food/water. Curves of prevalence, force of infection (FOI) and the number of new infections with 99% confidence intervals (CIs) were compared between the intermediate (North, Northeast, Midwest and Federal District) and low (South and Southeast) endemicity areas. A contour plot was also constructed.Results
The anti- HAV IgG seroprevalence was 68.8% (95% CI, 64.8%–72.5%) and 33.7% (95% CI, 32.4%–35.1%) for the intermediate and low endemicity areas, respectively, according to the field data analysis. The models showed that a higher force of infection was identified in the 10- to 19-year-old age cohort (∼9,000 infected individuals per year per 100,000 susceptible persons) in the intermediate endemicity area, whereas a higher force of infection occurred in the 15- to 29-year-old age cohort (∼6,000 infected individuals per year per 100,000 susceptible persons) for the other macro-regions.Conclusion
Our findings support the shift of Brazil toward intermediate and low endemicity levels with the shift of the risk of infection to older age groups. These estimates of HA force of infection stratified by age and endemicity levels are useful information to characterize the pre-vaccination scenario in Brazil. 相似文献79.
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