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排序方式: 共有446条查询结果,搜索用时 15 毫秒
441.
Raveendran M Harris RA Milosavljevic A Johnson Z Shelledy W Cameron J Rogers J 《Genomics》2006,88(6):706-710
Identification of polymorphic microsatellite loci in nonhuman primates is useful for various biomedical and evolutionary studies of these species. Prior methods for identifying microsatellites in nonhuman primates are inefficient. We describe a new strategy for marker development that uses the available whole genome sequence for rhesus macaques. Fifty-four novel rhesus-derived microsatellites were genotyped in large pedigrees of rhesus monkeys. Linkage analysis was used to place 51 of these loci into the existing rhesus linkage map. In addition, we find that microsatellites identified this way are polymorphic in other Old World monkeys such as baboons. This approach to marker development is more efficient than previous methods and produces polymorphisms with known locations in the rhesus genome assembly. Finally, we propose a nomenclature system that can be used for rhesus-derived microsatellites genotyped in any species or for novel loci derived from the genome sequence of any nonhuman primate. 相似文献
442.
Radoslav Aleksandrov Anton Dotchev Ina Poser Dragomir Krastev Georgi Georgiev Greta Panova Yordan Babukov Georgi Danovski Teodora Dyankova Lars Hubatsch Aneliya Ivanova Aleksandar Atemin Marina N. Nedelcheva-Veleva Susanne Hasse Mihail Sarov Frank Buchholz Anthony A. Hyman Stephan W. Grill Stoyno S. Stoynov 《Molecular cell》2018,69(6):1046-1061.e5
443.
Ivan Spasojevi Aleksandar Baji Katarina Jovanovi Mihajlo Spasi Pavle Andjus 《Carbohydrate research》2009,344(13):1676-1681
Astroglial cells represent the main line of defence against oxidative damage related to neurodegeneration. Therefore, protection of astroglia from an excess of reactive oxygen species could represent an important target of the treatment of such conditions. The aim of our study was to compare the abilities of glucose and fructose, the two monosaccharides used in diet and infusion, to protect C6 cells from hydrogen peroxide (H2O2)-mediated oxidative stress. It was observed using confocal microscopy with fluorescent labels and the MTT test that fructose prevents changes of oxidative status of the cells exposed to H2O2 and preserves their viability. Even more pronounced protective effects were observed for fructose 1,6-bis(phosphate). We propose that fructose and its intracellular forms prevent H2O2 from participating in the Fenton reaction via iron sequestration. As fructose and fructose 1,6-bis(phosphate) are able to pass the blood–brain barrier, they could provide antioxidative protection of nervous tissue in vivo. So, in contrast to the well-known negative effects of frequent consumption of fructose under physiological conditions, acute infusion or ingestion of fructose or fructose 1,6-bis(phosphate) could be of benefit in the cytoprotective therapy of neurodegenerative disorders related to oxidative stress. 相似文献
444.
Irena Miler Mihailo D. Rabasovic Marija Aleksic Aleksandar J. Krmpot Andjelika Kalezic Aleksandra Jankovic Bato Korac Aleksandra Korac 《Journal of biophotonics》2021,14(3):e202000362
Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) on 5 μm thick skin paraffin sections from 15-day-, 1-month- and 21-month-old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization-resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the β coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label-free method to previously fixed samples. 相似文献
445.
Aleksandar Vjetica Melvin Brard Gaowen Liu Laura Merlini Pedro Junior Nkosi Sophie G. Martin 《PLoS biology》2021,19(1)
To ensure genome stability, sexually reproducing organisms require that mating brings together exactly 2 haploid gametes and that meiosis occurs only in diploid zygotes. In the fission yeast Schizosaccharomyces pombe, fertilization triggers the Mei3-Pat1-Mei2 signaling cascade, which represses subsequent mating and initiates meiosis. Here, we establish a degron system to specifically degrade proteins postfusion and demonstrate that mating blocks not only safeguard zygote ploidy but also prevent lysis caused by aberrant fusion attempts. Using long-term imaging and flow-cytometry approaches, we identify previously unrecognized and independent roles for Mei3 and Mei2 in zygotes. We show that Mei3 promotes premeiotic S-phase independently of Mei2 and that cell cycle progression is both necessary and sufficient to reduce zygotic mating behaviors. Mei2 not only imposes the meiotic program and promotes the meiotic cycle, but also blocks mating behaviors independently of Mei3 and cell cycle progression. Thus, we find that fungi preserve zygote ploidy and survival by at least 2 mechanisms where the zygotic fate imposed by Mei2 and the cell cycle reentry triggered by Mei3 synergize to prevent zygotic mating.During sexual reproduction, fertilization must happen between exactly two gametes to ensure genome stability. This study shows that two mechanisms – establishment of zygotic fate and re-entry to the cell cycle – combine to prevent fission yeast zygotes fusing with further gametes. 相似文献
446.