全文获取类型
收费全文 | 6482篇 |
免费 | 452篇 |
专业分类
6934篇 |
出版年
2023年 | 53篇 |
2022年 | 89篇 |
2021年 | 202篇 |
2020年 | 168篇 |
2019年 | 173篇 |
2018年 | 246篇 |
2017年 | 241篇 |
2016年 | 301篇 |
2015年 | 331篇 |
2014年 | 397篇 |
2013年 | 461篇 |
2012年 | 491篇 |
2011年 | 459篇 |
2010年 | 301篇 |
2009年 | 251篇 |
2008年 | 355篇 |
2007年 | 328篇 |
2006年 | 310篇 |
2005年 | 247篇 |
2004年 | 228篇 |
2003年 | 172篇 |
2002年 | 204篇 |
2001年 | 121篇 |
2000年 | 135篇 |
1999年 | 83篇 |
1998年 | 56篇 |
1997年 | 31篇 |
1996年 | 27篇 |
1995年 | 44篇 |
1994年 | 16篇 |
1993年 | 17篇 |
1992年 | 39篇 |
1991年 | 31篇 |
1990年 | 20篇 |
1989年 | 20篇 |
1988年 | 32篇 |
1987年 | 26篇 |
1986年 | 16篇 |
1985年 | 21篇 |
1984年 | 19篇 |
1983年 | 13篇 |
1982年 | 17篇 |
1981年 | 11篇 |
1976年 | 10篇 |
1975年 | 9篇 |
1974年 | 10篇 |
1973年 | 11篇 |
1972年 | 9篇 |
1971年 | 10篇 |
1968年 | 8篇 |
排序方式: 共有6934条查询结果,搜索用时 15 毫秒
71.
Uedson Pereira Jacobina Marcelo Ricardo Vicari Pablo Ariel Martinez Marcelo de Bello Cioffi Luiz Antonio Carlos Bertollo Wagner Franco Molina 《Helgoland Marine Research》2013,67(3):499-506
Fish of the genus Selene, known as lookdowns or moonfish, are one of the most morphologically derived groups of the family Carangidae, whose phylogenetic relationships are still largely unknown. In this study, we discuss karyoevolutionary aspects of three representatives of this genus from the Western Atlantic: Selene brownii (2n = 48; FN = 48), Selene setapinnis (2n = 46; FN = 48), and Selene vomer (2n = 48; FN = 50). Their body patterns were also investigated and compared to one another and in relation to two other species of different genera. Two mechanisms of karyotypic evolution seem to have acted in the diversification of this genus, namely pericentric inversions and centric fusions. Mapping of rDNA sequences showed that chromosome pairs bearing 5S rDNA sites are similar, whereas those bearing 18 rDNA sites are morphologically distinct while apparently also exhibiting interspecies synteny. Although the nucleolar organizer-bearing chromosomes are extremely efficient cytotaxonomic markers among Selene species, others cytogenetic patterns of these species are relatively conserved. Hybridization with telomeric probes (TTAGGG)n did not exhibit interstitial telomeric sites (ITS), especially in S. setapinnis, where, along with a reduction in diploid number, a large metacentric pair derived from centric fusion is present. Data obtained by geometric morphometrics enable a clear morphological distinction among the three species, as well as in relation to two other species of the genus Caranx and Oligoplites. Data obtained suggest that morphologic evolution in Selene species was primarily dissociated from visible changes that occurred at the chromosomal level. 相似文献
72.
Sandro L. Pereira Mário Gr?os Ana Sofia Rodrigues Sandra I. Anjo Rui A. Carvalho Paulo J. Oliveira Ernest Arenas Jo?o Ramalho-Santos 《PloS one》2013,8(12)
The mitochondrion is emerging as a key organelle in stem cell biology, acting as a regulator of stem cell pluripotency and differentiation. In this study we sought to understand the effect of mitochondrial complex III inhibition during neuronal differentiation of mouse embryonic stem cells. When exposed to antimycin A, a specific complex III inhibitor, embryonic stem cells failed to differentiate into dopaminergic neurons, maintaining high Oct4 levels even when subjected to a specific differentiation protocol. Mitochondrial inhibition affected distinct populations of cells present in culture, inducing cell loss in differentiated cells, but not inducing apoptosis in mouse embryonic stem cells. A reduction in overall proliferation rate was observed, corresponding to a slight arrest in S phase. Moreover, antimycin A treatment induced a consistent increase in HIF-1α protein levels. The present work demonstrates that mitochondrial metabolism is critical for neuronal differentiation and emphasizes that modulation of mitochondrial functions through pharmacological approaches can be useful in the context of controlling stem cell maintenance/differentiation. 相似文献
73.
Rafaela T. P. Sant'Anna Emily V. Monteiro Juliano R. T. Pereira Roberto B. Faria 《PloS one》2013,8(12)
This work presents a new clock reaction based on ozone, iodine, and chlorate that differs from the known chlorate-iodine clock reaction because it does not require UV light. The induction period for this new clock reaction depends inversely on the initial concentrations of ozone, chlorate, and perchloric acid but is independent of the initial iodine concentration. The proposed mechanism considers the reaction of ozone and iodide to form HOI, which is a key species for producing non-linear autocatalytic behavior. The novelty of this system lies in the presence of ozone, whose participation has never been observed in complex systems such as clock or oscillating reactions. Thus, the autocatalysis demonstrated in this new clock reaction should open the possibility for a new family of oscillating reactions. 相似文献
74.
Anderson Messias Rodrigues Marcus de Melo Teixeira G. Sybren de Hoog Tania Maria Pacheco Schubach Sandro Antonio Pereira Geisa Ferreira Fernandes Leila Maria Lopes Bezerra Maria Sueli Felipe Zoilo Pires de Camargo 《PLoS neglected tropical diseases》2013,7(6)
Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. In order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1α) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Paraná (PR, n = 4), São Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. The genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. The occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil. 相似文献
75.
Stéphane Grison Gaëlle Favé Matthieu Maillot Line Manens Olivia Delissen Eric Blanchardon Nathalie Banzet Catherine Defoort Romain Bott Isabelle Dublineau Jocelyne Aigueperse Patrick Gourmelon Jean-Charles Martin Maâmar Souidi 《Metabolomics : Official journal of the Metabolomic Society》2013,9(6):1168-1180
Because uranium is a natural element present in the earth’s crust, the population may be chronically exposed to low doses of it through drinking water. Additionally, the military and civil uses of uranium can also lead to environmental dispersion that can result in high or low doses of acute or chronic exposure. Recent experimental data suggest this might lead to relatively innocuous biological reactions. The aim of this study was to assess the biological changes in rats caused by ingestion of natural uranium in drinking water with a mean daily intake of 2.7 mg/kg for 9 months and to identify potential biomarkers related to such a contamination. Subsequently, we observed no pathology and standard clinical tests were unable to distinguish between treated and untreated animals. Conversely, LC–MS metabolomics identified urine as an appropriate biofluid for discriminating the experimental groups. Of the 1,376 features detected in urine, the most discriminant were metabolites involved in tryptophan, nicotinate, and nicotinamide metabolic pathways. In particular, N-methylnicotinamide, which was found at a level seven times higher in untreated than in contaminated rats, had the greatest discriminating power. These novel results establish a proof of principle for using metabolomics to address chronic low-dose uranium contamination. They open interesting perspectives for understanding the underlying biological mechanisms and designing a diagnostic test of exposure. 相似文献
76.
Objectives
It is unclear whether sedentary behaviour, and the domain in which it occurs, is related to body mass index (BMI) change. We aim to elucidate whether sedentary behaviour is prospectively related to BMI change using markers from three domains (leisure, work and commuting).Methods
Among employed 1958 British birth cohort members (n = 6,562), we analysed whether TV-viewing, work sitting (six categories: 0 h/d to >4 h/d) and motorised commuting (at 45 y) were related to BMI (at 45 y and 50 y) and BMI change 45–50 y, after adjusting for lifestyle and socioeconomic factors.Results
Per category higher TV-viewing, 45 y and 50 y BMI were higher by 0.69 kg/m2 (95% CI: 0.59,0.80) and 0.75 kg/m2 (0.64,0.86) respectively. A category higher TV-viewing was associated with 0.11 kg/m2 (0.06,0.17) increased BMI 45–50 y, attenuating to 0.06 kg/m2 (0.01,0.12) after adjustment. There was no trend for work sitting with 45 y or 50 y BMI, nor, after adjustment, for BMI change. However, those sitting 2–3 h/d had greater BMI gain by 0.33 kg/m2 (0.10,0.56) compared to those sitting 0–1 h/d. Associations between TV-viewing and BMI change were independent of work sitting. Motorised commuting was associated with 45 y, but not 50 y BMI or change.Conclusions
TV-viewing is associated with BMI gain in mid-adulthood; evidence is weaker for other sedentary behaviours. 相似文献77.
David N. Gumieiro Bruna P. M. Rafacho Andrea F. Gon?alves Priscila P. Santos Paula S. Azevedo Leonardo A. M. Zornoff Gilberto J. C. Pereira Luiz S. Matsubara Sergio A. R. Paiva Marcos F. Minicucci 《PloS one》2013,8(2)
Introduction
The aim of this study is to evaluate the serum activity of metalloproteinases (MMPs) -2 and -9 as predictors of pressure ulcer (PU), gait status and mortality 6 months after hip fracture.Methods
Eighty-seven patients over the age of 65 admitted to the orthopedic unit from January to December 2010 with hip fracture were prospectively evaluated. Upon admission, patient demographic information, including age, gender and concomitant diseases, was recorded. Blood samples were taken for analysis of MMP -2 and -9 activity by gel zymography and for biochemical examination within the first 72 hours of the patient’s admission, after clinical stabilization. The fracture pattern (neck, trochanteric or subtrochanteric), time from admission to surgery, surgery duration and length of hospital stay were also recorded.Results
Two patients were excluded due to the presence of pathological fractures (related to cancer), and three patients were excluded due to the presence of PU before admission. Eighty-two patients, with a mean age of 80.4 ± 7.3 years, were included in the analysis. Among these patients, 75.6% were female, 59.8% had PU, and 13.4% died 6 months after hip fracture. All patients underwent hip fracture repair. In a univariate analysis, there were no differences in serum MMP activity between hip fracture patients with or without PU. In addition, the multiple logistic regression analysis models, which were adjusted by age, gender, length of hospital stay and C-reactive protein, showed that the pro-MMP-9 complexed with neutrophil gelatinase-associated lipocalin form (130 kDa) was associated with gait status recovery 6 months after hip fracture.Conclusions
In conclusion, serum pro-MMP-9 is a predictor of gait status recovery 6 months after hip fracture. 相似文献78.
da Silva-Carvalho Lidiane Maria Genovez-Oliveira Jhon Lennon de Souza Oliveira Mariana de Oliveira Águida Aparecida de Lima Viviane Moreira Ferreira Ildemar Berto Bruno Pereira 《Systematic parasitology》2020,97(6):847-855
Systematic Parasitology - Isospora parnaitatiaiensis Silva, Rodrigues, Lopes, Berto, Luz, Ferreira & Lopes, 2015 was identified from a new host, the plain antvireo Dysithamnus mentalis... 相似文献
79.
Marcelo F. G. Brito Ayda V. Alcântara Daniela C. O. Rosa Bruno E. Soares 《Zeitschrift fur angewandte Ichthyologie》2020,36(5):753-756
Length-weight relationships (LWR) and length-length relationships (LLR) were provided for eight species inhabiting Northeastern Brazilian coast. Nine transects (5, 15, 30 m deep) between Sergipe and Vaza-Barris rivers mouths were sampled monthly from September 2013 to August 2014. Each sampling was carried out using a shrimp boat equipped with double ring nets (4 m mouth wide, 10 m long and 20 mm mesh size) and trawlings were performed for 15 min. Fishes were measured (standard and total length, 0.1 cm) and weighted (total weight, 0.01 g). We provide LWR and LLR coefficients for these eight species, including new maximum TL for five species, and discuss the similarity of these results with estimates available in Fishbase. 相似文献
80.
Gehring Christoph Zelarayán Marcelo Correa Luz Ronildson Lima Almeida Rosângela Borges Boddey Robert Michael Leite Márcio Fernandes Alves 《Plant and Soil》2020,454(1-2):447-460
Plant and Soil - Aggressive ruderal plant species pose an increasing problem in anthropically degraded lands. They both affect and are affected by other vegetation and by soil fertility in their... 相似文献