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81.
Carlos Alberto da Silva Silvia de Oliveira Santos Carlos Alexandre Borges Garcia Gabriela Cugler de Pontes 《人类与生态风险评估》2020,26(3):695-712
AbstractArsenic, cadmium, lead, and mercury in fish is the result of long-term biomagnification in the food chain and is of public concern, due to the toxicity they engender. The objective of this research was to determine the concentrations of arsenic, cadmium, lead, and mercury in 13 species of marine fish broadly commercialized in Aracaju, SE, Brazil and to evaluate the risks of fish consumption associated with these trace elements, using the Target Hazard Quotient (THQ). As, Cd, and Pb levels were measured with inductively coupled plasma mass spectrometry (ICP-MS), and mercury was analyzed via cold vapor atomic absorption spectrometry. The results indicate a large variability in concentrations for arsenic (0.07–2.03?mg kg–1) and mercury (0.01–1.44?mg kg–1), associated with the animal dietary category. Cadmium (0.04–0.19?mg kg–1) and lead (<0.01–0.45?mg kg–1), on the other hand showed a mild variability. None of the evaluated specimens had As, Cd, and Pb THQ values higher than 1. The THQ values for mercury were higher but indicated no consumption risk, except for amberjack, and snook fish. Overall THQ indicates lower risk of consumption in fish that are at the base of the food chain, than in those that are top predators. 相似文献
82.
Saraiva DG Fournier GF Martins TF Leal KP Vieira FN Câmara EM Costa CG Onofrio VC Barros-Battesti DM Guglielmone AA Labruna MB 《Experimental & applied acarology》2012,58(2):159-166
From June 2005 to November 2010, 43 small mammals encompassing 6 species of Didelphimorphia, 8 species of Rodentia, and 1 species of Lagomorpha were found parasitized by ticks in the state of Minas Gerais, southeastern Brazil. Nine tick species, in total 186 specimens, were identified as follows: Amblyomma cajennense (larvae and nymphs) on opossums and rodents; Amblyomma ovale (nymphs) on rodents; Amblyomma parvum (nymphs) on rodents; Amblyomma coelebs (nymphs) on opossums; Amblyomma dubitatum (nymph) on opossums; Ixodes amarali (females, nymphs, and larvae) on opossums and rodents; Ixodes loricatus (male, females, nymph) on opossums; Ixodes schulzei (female) on rodents; and Haemaphysalis leporispalustris (female) on rabbits. Most of the tick-host associations found in the present study have never been recorded in the literature; those include three new host records for I. amarali, four for A. cajennense, one for A. dubitatum, two for A. ovale, and one for A. coelebs. In addition, we provide the first record of A. coelebs in the state of Minas Gerais. 相似文献
83.
Manihot alterniflora and M. elongata spp. nov. (Euphorbiaceae) and the rediscovery of M. quinquefolia in Caatinga (semiarid) vegetation in Brazil 下载免费PDF全文
Márcio Lacerda Lopes Martins Paulo Cezar Lemos de Carvalho Carlos Alberto da Silva Ledo André Márcio Amorim 《Nordic Journal of Botany》2018,36(3)
We describe and illustrate two species of Manihot that occur in Caatinga (semiarid) vegetation in Brazil and redescribe and lectotypify M. quinquefolia Pohl, which was only known from a single collection made by J. E. B. Pohl in 1827. Manihot elongata P.Carvalho & M.Martins is widely distributed and Manihot alterniflora P.Carvalho & M.Martins is endangered because of its small populations and restricted area of occurrence. We establish M. quinquefolia as the only species of Manihot in the Caatinga with compound leaves. An identification key is provided for the 13 species of Manihot present in the Caatinga. 相似文献
84.
Influence of the margin vegetation on the conservation of aphid biological control in apple orchards
Luan Alberto Odorizzi Santos Marlice Botelho Costa Claire Lavigne Odair Aparecido Fernandes Armin Bischoff Pierre Franck 《Journal of Insect Conservation》2018,22(3-4):465-474
The influence of three margin strip treatments (wildflower strips, grass strips and spontaneous vegetation) adjacent to apple orchards on the biological control of Dysaphis plantaginea Passerini (Hemiptera: Aphididae) was compared during two consecutive years. The wildflower strips provided the highest amount of floral resources. Within the margin strips, hoverflies responded positively to higher resource provisioning whereas ladybird abundance did not differ between strip treatments. Within the orchards, the presence of parasitoids, hoverflies, and ladybirds in aphid colonies and the predation of sentinel aphids were not significantly affected by the adjacent strip treatments. The number of natural enemies observed in aphid colonies was mainly driven by aphid number. Aphid numbers were higher close to the margin strips suggesting that aphid colonization from orchard edges may counteract the positive effect of wildflower strips on natural enemy abundance and on a reduction of aphid infestation. The results confirm the positive influence of floral resource provisioning by wildflower strips on the conservation of aphid natural enemies, but also suggest that effects of wildflower strips on aphid regulation inside orchards are not very strong compared with spontaneous vegetation naturally occurring in the margins. 相似文献
85.
Claudio Napoli Alberto Zullo Antonietta Picascia Teresa Infante Francesco Paolo Mancini 《Journal of cellular biochemistry》2013,114(1):7-20
In recent years, the diagnosis of cardiovascular disease (CVD) has increased its potential, also thanks to mass spectrometry (MS) proteomics. Modern MS proteomics tools permit analyzing a variety of biological samples, ranging from single cells to tissues and body fluids, like plasma and urine. This approach enhances the search for informative biomarkers in biological samples from apparently healthy individuals or patients, thus allowing an earlier and more precise diagnosis and a deeper comprehension of pathogenesis, development and outcome of CVD to further reduce the enormous burden of this disease on public health. In fact, many differences in protein expression between CVD‐affected and healthy subjects have been detected, but only a few of them have been useful to establish clinical biomarkers because they did not pass the verification and validation tests. For a concrete clinical support of MS proteomics to CVD, it is, therefore, necessary to: ameliorate the resolution, sensitivity, specificity, throughput, precision, and accuracy of MS platform components; standardize procedures for sample collection, preparation, and analysis; lower the costs of the analyses; reduce the time of biomarker verification and validation. At the same time, it will be fundamental, for the future perspectives of proteomics in clinical trials, to define the normal protein maps and the global patterns of normal protein levels, as well as those specific for the different expressions of CVD. J. Cell. Biochem. 114: 7–20, 2012. © 2012 Wiley Periodicals, Inc. 相似文献
86.
Rosa Sorrentino Carlo Iannicola Sandro Costanzi Alberto Chersi Roberto Tosi 《Immunogenetics》1991,33(2):118-123
DNA molecules derived from three alleles of the HLA-DRB3 locus and differing from each other at several nucleotide sites were denatured and cross-hybridized. Each allelic combination was found to generate a pair of heteroduplexes of different mobility. Their retardation as compared to homoduplexes was proportional to the number of mismatches. In each heteroduplexes pair the component possessing the highest number of Pyr-Pyr oppositions was the most retarded. The results are those predicted by a theoretical model implying a correlation between base-pair opening and bending of the DNA double helix. These observations introduce a new HLA typing method at the genomic level and indicate an experimental approach to the analysis of the superhelical DNA conformation as related to different types of base oppositions. 相似文献
87.
88.
Vdo C Souza KB Schlemmer CB Noal JA Jaques CE Zimmermann CA Leal J Fleck EA Casali VM Morsch MR Schetinger DB Leal 《Parasitology international》2012,61(4):690-696
Trypanosoma cruzi infection triggers a chronic inflammatory process in human host and purinergic system ecto-enzymes play an important role in modulating the inflammatory and immune responses. In this study, it was investigated ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and ecto-adenosine deaminase (E-ADA; EC 3.5.4.4) activities in lymphocytes from patients with indeterminate form of Chagas' disease (IFCD). Twenty-five IFCD patients and 25 healthy subjects (control group) were selected. The peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Adenine nucleotides and adenosine levels were determined in serum by HPLC and the E-NTPDase1 expression in lymphocytes by Western blot analysis. E-NTPDase (ATP and ADP as substrates) and E-ADA (adenosine as substrate) activities were decreased in lymphocytes from IFCD patients (P<0.05 and P<0.01, respectively), while the E-NTPDase1 expression presented no changes in these patients. Serum ATP levels showed to be decreased (P<0.05) and both AMP (P<0.01) and adenosine (P<0.001) levels were increased in the IFCD group. The enzymatic alterations observed are in agreement with the immune response against T. cruzi infection in IFCD patients, since the decreased extracellular ATP and the increased adenosine levels trigger a Th2 anti-inflammatory response, which it is associated to adaptation of host to parasite, preventing clinical progress of disease. 相似文献
89.
Alberto Anzures-Dadda Ellen Andresen María Luisa Martínez Robert H. Manson 《International journal of primatology》2011,32(3):634-651
The disappearance of frugivorous primates in fragmented forests can potentially lower the rates of seed dispersal and recruitment
of endozoochorous tree species, thus altering plant community structure. We quantified seedling density for 7 tree species
that are common in the feces of mantled howlers (Alouatta palliata) in 6 rain forest fragments in northern Chiapas, Mexico. Howlers were present in 3 of the fragments and absent in the other
3. We compared seedling density in primate sleeping sites in inhabited fragments with control sites, which were structurally
similar to sleeping sites but where we did not find monkey feces, in both inhabited and uninhabited fragments. For each tree
species, we determined the relationship between seedling density and the local density of seeds and adult trees. In fragments
where howlers were present, seedling density for 4 of the focal tree species (Brosimum alicastrum, Dialium guianense, Manilkara zapota, and Nectandra ambigens) was greater in sleeping sites than in control sites found in the same fragments. Moreover, seedling density of Dialium guianense was greater in the control sites of fragments inhabited by howlers than in fragments where this primate is absent. Seedling
density of these 4 species correlates positively with seed density on the forest floor; however, we observed no correlations
between seedling density and the density of adult trees. Our results suggest that the diversity of the seedling community
of tree species dispersed by howlers may decline in fragments where this seed disperser is absent. These findings, together
with the fact that only 5% of the study region is currently covered by forest and 81% of the forest remnants are uninhabited
by mantled howlers, suggest that the potential long-term recovery of associated populations of tropical tree species dispersed
by this primate species is highly uncertain. Conservation and restoration efforts should be aimed at restoring or replacing
the ecological role played by this important seed disperser in the region. 相似文献
90.
Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis 总被引:2,自引:0,他引:2
Cassatella MA Pereira-da-Silva G da Silva GP Tinazzi I Facchetti F Scapini P Calzetti F Tamassia N Wei P Nardelli B Roschke V Vecchi A Mantovani A Bambara LM Edwards SW Carletto A 《Journal of immunology (Baltimore, Md. : 1950)》2007,178(11):7325-7333
TNF-like cytokine (TL1A) is a newly identified member of the TNF superfamily of ligands that is important for T cell costimulation and Th1 polarization. However, despite increasing information about its functions, very little is known about expression of TL1A in normal or pathological states. In this study, we report that mononuclear phagocytes appear to be a major source of TL1A in rheumatoid arthritis (RA), as revealed by their strong TL1A expression in either synovial fluids or synovial tissue of rheumatoid factor (RF)-seropositive RA patients, but not RF-/RA patients. Accordingly, in vitro experiments revealed that human monocytes express and release significant amounts of soluble TL1A when stimulated with insoluble immune complexes (IC), polyethylene glycol precipitates from the serum of RF+/RA patients, or with insoluble ICs purified from RA synovial fluids. Monocyte-derived soluble TL1A was biologically active as determined by its capacity to induce apoptosis of the human erythroleukemic cell line TF-1, as well as to cooperate with IL-12 and IL-18 in inducing the production of IFN-gamma by CD4(+) T cells. Because RA is a chronic inflammatory disease with autoimmune etiology, in which ICs, autoantibodies (including RF), and various cytokines contribute to its pathology, our data suggest that TL1A could be involved in its pathogenesis and contribute to the severity of RA disease that is typical of RF+/RA patients. 相似文献