全文获取类型
收费全文 | 123746篇 |
免费 | 8355篇 |
国内免费 | 236篇 |
出版年
2023年 | 757篇 |
2022年 | 900篇 |
2021年 | 2065篇 |
2020年 | 1618篇 |
2019年 | 1851篇 |
2018年 | 3329篇 |
2017年 | 2961篇 |
2016年 | 4060篇 |
2015年 | 5495篇 |
2014年 | 5653篇 |
2013年 | 7586篇 |
2012年 | 8812篇 |
2011年 | 8168篇 |
2010年 | 5209篇 |
2009年 | 4420篇 |
2008年 | 6435篇 |
2007年 | 6168篇 |
2006年 | 5885篇 |
2005年 | 5064篇 |
2004年 | 5021篇 |
2003年 | 4513篇 |
2002年 | 4196篇 |
2001年 | 3027篇 |
2000年 | 2912篇 |
1999年 | 2328篇 |
1998年 | 1170篇 |
1997年 | 874篇 |
1996年 | 835篇 |
1995年 | 855篇 |
1994年 | 693篇 |
1993年 | 641篇 |
1992年 | 1352篇 |
1991年 | 1260篇 |
1990年 | 1090篇 |
1989年 | 1114篇 |
1988年 | 1018篇 |
1987年 | 981篇 |
1986年 | 885篇 |
1985年 | 895篇 |
1984年 | 806篇 |
1983年 | 649篇 |
1982年 | 531篇 |
1981年 | 452篇 |
1979年 | 644篇 |
1978年 | 519篇 |
1975年 | 478篇 |
1974年 | 543篇 |
1973年 | 551篇 |
1972年 | 473篇 |
1970年 | 447篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
62.
The production of L-lysine fromDL-α-amino-ε-caprolactam (DL-ACL) by new strains producingL-α-amino-ε-caprolactamase and aminocaprolactam racemase is described. Optimal conditions for hydrolysis ofL-ACL byCryptococcus sp. and for racemization of ACL by cells of a strain isolated in nature and identified asPseudomonas sp. were determined. Synthesis ofL-α-amino-ε-caprolactamase is induced byDL-ACL orL-lysine with the same effectivity. A positive effect of phosphates (potassium salts) on reduction of the induction lag was
detected, the synthesis of this enzyme was found to be repressed by glucose and some possibilities of the reversion of this
repressive effect were demonstrated. Under conditions optimal for the production of both enzymes a quantitative theoretical
conversion of 10 % aqueousDL-ACL toL-lysine by a mixture of native cells in a mass ratio of 1: 2 (producer of ACL-hydrolase to producer of ACL-racemase) occurred
in 8 h at 40 °C and pH 8.0 相似文献
63.
64.
65.
F García J Sánchez J Planas 《Comparative biochemistry and physiology. A, Comparative physiology》1987,87(4):947-950
1. Male quails submitted 20 and 120 days to a low iron diet (7 ppm) were compared to female laying quails, exposed for 30 days to the same low iron regime, in order to compare the response of the iron metabolic control under a single (erythropoiesis) or a doubled (erythropoiesis and egg formation) iron demand. 2. Iron deposit in storage organs, the classical hematology and the intestinal iron absorption were analyzed in these animals. 3. In males, after 120 days, the iron deposits were reduced 50 and 75%, but hematological values (hematocrit and hemoglobin concentration) were normal, although in laying quails, after 30 days, an anemic condition was evident in both blood parameters and iron deposits, provoking an iron deficient erythropoiesis. 4. The enhancement of the intestinal iron uptake, confirms the anemic character of these birds. 相似文献
66.
Xiaozhong Huang Yujuan Shi Hongjin Chen Rongrong Le Xiaohua Gong Ke Xu Qihan Zhu Feixia Shen Zimiao Chen Xuemei Gu Xiaojun Chen Xiong Chen 《Cell death & disease》2020,11(12)
Diabetic nephropathy (DN) as a global health concern is closely related to inflammation and oxidation. Isoliquiritigenin (ISL), a natural flavonoid compound, has been demonstrated to inhibit inflammation in macrophages. Herein, we investigated the effect of ISL in protecting against the injury in STZ-induced type 1 DN and in high glucose-induced NRK-52E cells. In this study, it was revealed that the administration of ISL not only ameliorated renal fibrosis and apoptosis, but also induced the deterioration of renal function in diabetic mice. Mediated by MAPKs and Nrf-2 signaling pathways, respectively, upstream inflammatory response and oxidative stress were neutralized by ISL in vitro and in vivo. Moreover, as further revealed by the results of molecular docking, sirtuin 1 (SIRT1) binds to ISL directly, and the involvement of SIRT1 in ISL-mediated renoprotective effects was confirmed by studies using in vitro models of SIRT1 overexpression and knockdown. In summary, by reducing inflammation and oxidative stress, ISL has a significant pharmacological effect on the deterioration of DN. The benefits of ISL are associated with the direct binding to SIRT1, the inhibition of MAPK activation, and the induction of Nrf-2 signaling, suggesting the potential of ISL for DN treatment.Subject terms: Pharmacology, Molecular biology 相似文献
67.
A Sólyom S Tóth I Holczinger J Vajda Z Tóth R Kálmánchey 《Applied neurophysiology》1985,48(1-6):222-225
Four thalamic and cortical recordings were carried out in 5 patients. The thalamic-evoked potentials were typical and revealed a triphasic complex, but their latencies showed a relatively high standard deviation. They could be divided into two groups according to their latencies, both of which had low SD. These data suggested that there could be two types of latency of thalamic SEP, because the 4 patients' body sizes were very similar. More detailed surface, cortical and depth recordings are needed to resolve these questions. 相似文献
68.
Genetically modified Saccharomyces cerevisiae strain (YPB-G) which secretes a bifunctional fusion protein that contains both Bacillus subtilis -amylase and Aspergillus awamori glucoamylase activities was used for the direct conversion of starch into ethanol. Starch was either supplied initially to different nutrient media or added instantaneously to the reactor at various discrete time instants (pulse feeding). Stoichiometric modeling was used to investigate the effects of initial substrate concentration and growth rate of the recombinant yeast culture on ethanol production. Reaction stoichiometries describing both the anabolism and catabolism of the microorganism were used as an input to flux balance analysis (FBA), the preferred metabolic modeling approach since the constructed stoichiometric network was underdetermined. Experiments for batch and fed-batch systems at different substrate concentrations were analyzed theoretically in terms of flux distributions using ethanol production rate as the maximization criteria. Calculated ethanol rates were in agreement with experimental measurements, suggesting that this recombinant microorganism is sufficiently evolved to optimize its ethanol production. The function of the main pathways of yeast metabolism (PPP, EMP, TCA) are discussed together with the node analyses of glucose-6-P and pyruvate branch points. Theoretical node analysis revealed that if the split ratio in G6P branch point is changed by genetic manipulations, the ethanol yield would be affected considerably. 相似文献
69.
70.
Effect of precursors on biosynthesis of monensins A and B 总被引:1,自引:0,他引:1
Precursors of monensins (acetate, propionate, butyrate, isobutyrate) affect the total production and the relative proportion of monensins A and B. Addition of propionate into the fermentation medium causes a prevalence of monensin B whereas butyrate and isobutyrate stimulate the production of monensin A and suppress the production of monensin B. 相似文献