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131.
Maren de Vries Adil S. Mohamed Rachel A. Prescott Ana M. Valero-Jimenez Ludovic Desvignes Rebecca OConnor Claire Steppan Joseph C. Devlin Ellie Ivanova Alberto Herrera Austin Schinlever Paige Loose Kelly Ruggles Sergei B. Koralov Annaliesa S. Anderson Joseph Binder Meike Dittmann 《Journal of virology》2021,95(10)
132.
Langeneck Joachim Englezou Chris Di Maggio Matteo Castelli Alberto Maltagliati Ferruccio 《Hydrobiologia》2021,848(17):4093-4114
Hydrobiologia - Aphanius fasciatus is a small fish occurring in Mediterranean brackish environments. In Cyprus it is known from three localities separated by long stretches of coast. The genetic... 相似文献
133.
Fadhlaoui-Zid Karima Cossu Piero Sanna Daria Scarpa Fabio Lai Tiziana Castelli Alberto Casu Marco Maltagliati Ferruccio 《Hydrobiologia》2021,848(18):4225-4240
Hydrobiologia - Recent research hypothesised that the Siculo-Tunisian Strait might fit, at least for some species, the picture of a genetic transitional zone instead of a sharp genetic break... 相似文献
134.
Eidi Kikuchi Sebastían García Paulo Alberto Silva da Costa Luis Gustavo Cardoso Manuel Haimovici 《Journal of fish biology》2021,98(2):548-556
Otolith shape analysis is a powerful method for fish stock identification. We compared the otolith shape of Pagrus pagrus (Linnaeus 1758) along with its distribution in four south-western Atlantic regions where it is commercially fished: Rio de Janeiro, Rio Grande do Sul in southern Brazil, the Argentine-Uruguayan Common Fishing Zone (UA) and the Argentinian Exclusive Fishing Zone (AR). Otolith shapes were compared by Elliptical Fourier and Wavelet coefficients among specimens in a size range with similar otoliths, morphometric parameters and ages. Four potential stocks were identified: one in the AR, a second along the UA which included specimens from southern Brazil with well-marked opaque bands in its otoliths (MRS), the third in southern Brazil with faint or absent opaque bands in its otoliths (FRS) and the fourth along Rio de Janeiro. The difference in the otolith shape among regions followed differences reported using other stock identification techniques. The similarity between otoliths from UA and MRS (ANOVA-like, P > 0.01) can be explained by seasonal short-range migrations. Otoliths shape differences between MRS and FRS (ANOVA-like, P < 0.01) suggest that P. pagrus does not form a homogeneous group in southern Brazil. 相似文献
135.
136.
Alberto Cornejo Julio Caballero Mario Simirgiotis Vanessa Torres Luisa Snchez Nicols Díaz Marcela Guimaraes Marcos Hernndez Carlos Areche Sergio Alfaro Leonardo Caballero Francisco Melo 《Journal of enzyme inhibition and medicinal chemistry》2021,36(1):154
Parkinson''s disease (PD) is a neurodegenerative disorder that affects adult people whose treatment is palliative. Thus, we decided to test three dammarane triterpenes 1, 1a, 1b, and we determined that 1 and 1a inhibit β-aggregation through thioflavine T rather than 1b. Since compound 1 was most active, we determined the interaction between α-synuclein and 1 at 50 µM (Kd) through microscale thermophoresis. Also, we observed differences in height and diameter of aggregates, and α-synuclein remains unfolded in the presence of 1. Also, aggregates treated with 1 do not provoke neurites'' retraction in N2a cells previously induced by retinoic acid. Finally, we studied the potential sites of interaction between 1 with α-synuclein fibrils using molecular modelling. Docking experiments suggest that 1 preferably interact with the site 2 of α-synuclein through hydrogen bonds with residues Y39 and T44. 相似文献
137.
Joy H. Meserve Jessica C. Nelson Kurt C. Marsden Jerry Hsu Fabio A. Echeverry Roshan A. Jain Marc A. Wolman Alberto E. Pereda Michael Granato 《PLoS genetics》2021,17(6)
The acoustic startle response is an evolutionarily conserved avoidance behavior. Disruptions in startle behavior, particularly startle magnitude, are a hallmark of several human neurological disorders. While the neural circuitry underlying startle behavior has been studied extensively, the repertoire of genes and genetic pathways that regulate this locomotor behavior has not been explored using an unbiased genetic approach. To identify such genes, we took advantage of the stereotypic startle behavior in zebrafish larvae and performed a forward genetic screen coupled with whole genome analysis. We uncovered mutations in eight genes critical for startle behavior, including two genes encoding proteins associated with human neurological disorders, Dolichol kinase (Dolk), a broadly expressed regulator of the glycoprotein biosynthesis pathway, and the potassium Shaker-like channel subunit Kv1.1. We demonstrate that Kv1.1 and Dolk play critical roles in the spinal cord to regulate movement magnitude during the startle response and spontaneous swim movements. Moreover, we show that Kv1.1 protein is mislocalized in dolk mutants, suggesting they act in a common genetic pathway. Combined, our results identify a diverse set of eight genes, all associated with human disorders, that regulate zebrafish startle behavior and reveal a previously unappreciated role for Dolk and Kv1.1 in regulating movement magnitude via a common genetic pathway. 相似文献
138.
Liliane Bezerra de Lima Wliana Alves Viturino da Silva Ewelyn Cintya Felipe dos Santos Janaína Carla Barbosa Machado Thamara Figueiredo Procópio Maiara Celine de Moura Thiago Henrique Napoleão Magda Rhayanny Assunção Ferreira Luiz Alberto Lira Soares 《化学与生物多样性》2021,18(12):e2100538
The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential of crude extract and fractions of Punica granatum leaves. The extract was produced by turbo extraction, after which hexanic, ethyl acetate, and aqueous fractions were obtained by partitioning. The chemical analyses were performed by thin layer chromatography and high-performance liquid chromatography, and the antioxidant activities were assayed by DPPH. and ABTS.+. Minimal inhibitory and bactericidal concentrations (MIC/MBC) were applied to twenty-two bacteria. Most strains susceptible to extract/fractions and resistant to antibiotics were selected, and ampicillin, azithromycin, ciprofloxacin, and gentamicin were associated with the ethyl acetate fraction (EAF) against multidrug-resistant strains in modulatory and checkboard models. The data from chromatographic analyses showed flavonoids and tannins in the extract, as well as the enrichment of EAF in phenols, mainly flavonoids. The flavonoids were connected to the electron transfer activity demonstrated in the DPPH. and ABTS.+ assays. Gram-positive strains are more susceptible to EAF. The subinhibitory concentrations of P. granatum enhanced the antimicrobial activity of the agents and reduced the EAF individual MIC, and the combination of EAF and antibiotics demonstrated a synergistic effect. These results present a promising approach for developing a therapy in which antioxidant extracts and fractions can be used in combination with antibiotics. 相似文献
139.
Alonso Alberto Pérez Javier Monroy Silvia López-Rojo Naiara Basaguren Ana Bosch Jaime Boyero Luz 《Ecosystems》2021,24(6):1436-1449
Ecosystems - Leaf litter of alder (Alnus glutinosa) is a key resource to detrital stream food webs. Due to its high quality and palatability, it is readily colonised by microorganisms and consumed... 相似文献
140.
Alejandro Ramirez Garcia Jianbo Zhang Anna Greppi Florentin Constancias Esther Wortmann Muriel Wandres Katherine Hurley Alberto Pascual-García Hans-Joachim Ruscheweyh Shana J. Sturla Christophe Lacroix Clarissa Schwab 《Environmental microbiology》2021,23(3):1765-1779
Glycerol/diol dehydratases (GDH) are enzymes that catalyse the production of propionate from 1,2-propanediol, and acrolein from glycerol. Acrolein reacts with dietary carcinogenic heterocyclic amines (HCA), reducing HCA mutagenicity, but is itself also an antimicrobial agent and toxicant. Gut microbial GDH activity has been suggested as an endogenous acrolein source; however, there is limited information on the potential of the intestinal microbiota to have GDH activity, and what impact it can have on the intestinal ecosystem and host health. We hypothesized that GDH activity of gut microbiota is determined by the abundance and distribution of GDH-active taxa and can be enhanced by supplementation of the GDH active Anaerobutyricum hallii, and tested this hypothesis combining quantitative profiling of gdh, model batch fermentations, microbiota manipulation, and kinetic modelling of acrolein formation. Our results suggest that GDH activity is a common trait of intestinal microbiota shared by a few taxa, which was dependent on overall gdh abundance. Anaerobutyricum hallii was identified as a key taxon in GDH metabolism, and its supplementation increased the rate of GDH activity and acrolein release, which enhanced the transformation of HCA and reduced fermentation activity. The findings of this first systematic study on acrolein release by intestinal microbiota indicate that dietary and microbial modulation might impact GDH activity, which may influence host health. 相似文献