全文获取类型
收费全文 | 253篇 |
免费 | 23篇 |
出版年
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 6篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 12篇 |
2014年 | 6篇 |
2013年 | 19篇 |
2012年 | 31篇 |
2011年 | 20篇 |
2010年 | 12篇 |
2009年 | 14篇 |
2008年 | 12篇 |
2007年 | 6篇 |
2006年 | 16篇 |
2005年 | 11篇 |
2004年 | 6篇 |
2003年 | 11篇 |
2002年 | 8篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1995年 | 3篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1954年 | 1篇 |
1925年 | 1篇 |
1924年 | 1篇 |
1922年 | 1篇 |
1919年 | 1篇 |
1916年 | 2篇 |
1915年 | 3篇 |
1914年 | 2篇 |
1909年 | 1篇 |
1908年 | 2篇 |
1905年 | 3篇 |
1904年 | 1篇 |
1902年 | 1篇 |
1901年 | 1篇 |
1900年 | 1篇 |
1899年 | 3篇 |
1897年 | 2篇 |
1892年 | 2篇 |
1889年 | 2篇 |
1887年 | 1篇 |
1877年 | 1篇 |
排序方式: 共有276条查询结果,搜索用时 31 毫秒
231.
Claire Toffano-Nioche An N. Nguyen Claire Kuchly Alban Ott Daniel Gautheret Philippe Bouloc Annick Jacq 《RNA (New York, N.Y.)》2012,18(12):2201-2219
Work in recent years has led to the recognition of the importance of small regulatory RNAs (sRNAs) in bacterial regulation networks. New high-throughput sequencing technologies are paving the way to the exploration of an expanding sRNA world in nonmodel bacteria. In the Vibrio genus, compared to the enterobacteriaceae, still a limited number of sRNAs have been characterized, mostly in Vibrio cholerae, where they have been shown to be important for virulence, as well as in Vibrio harveyi. In addition, genome-wide approaches in V. cholerae have led to the discovery of hundreds of potential new sRNAs. Vibrio splendidus is an oyster pathogen that has been recently associated with massive mortality episodes in the French oyster growing industry. Here, we report the first RNA-seq study in a Vibrio outside of the V. cholerae species. We have uncovered hundreds of candidate regulatory RNAs, be it cis-regulatory elements, antisense RNAs, and trans-encoded sRNAs. Conservation studies showed the majority of them to be specific to V. splendidus. However, several novel sRNAs, previously unidentified, are also present in V. cholerae. Finally, we identified 28 trans sRNAs that are conserved in all the Vibrio genus species for which a complete genome sequence is available, possibly forming a Vibrio “sRNA core.” 相似文献
232.
Erratum: The kynurenine pathway is activated in human obesity and shifted toward kynurenine monooxygenase activation
下载免费PDF全文
![点击此处可从《Obesity (Silver Spring, Md.)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Marie Favennec Benjamin Hennart Robert Caiazzo Audrey Leloire Loïc Yengo Marie Verbanck Abdelilah Arredouani Michel Marre Marie Pigeyre Alban Bessede Gilles J. Guillemin Giulia Chinetti Bart Staels François Pattou Beverley Balkau Delphine Allorge Philippe Froguel Odile Poulain‐Godefroy 《Obesity (Silver Spring, Md.)》2016,24(8):1821-1821
233.
NtRING1, putative RING-finger E3 ligase protein,is a positive regulator of the early stages of elicitin-induced HR in tobacco 总被引:1,自引:0,他引:1
Ahmed Ghannam Alban Jacques Patrice de Ruffray Serge Kauffmann 《Plant cell reports》2016,35(2):415-428
Key message
NtRING1 is a RING-finger protein with a putative E3 ligase activity. NtRING1 regulates HR establishment against different pathogens. Loss-/gain-of-function of NtRING1 altered early stages of HR phenotype establishment.Abstract
Plant defence responses against pathogens often involve the restriction of pathogens by inducing a hypersensitive response (HR). cDNA clones DD11-39, DD38-11 and DD34-26 were previously obtained from a differential screen aimed at characterising tobacco genes with an elicitin-induced HR-specific pattern of expression. Our precedent observations suggested that DD11-39, DD38-11 and DD34-26 might play roles in the HR establishment. Only for DD11-39 a full-length cDNA sequence was obtained and the corresponding protein encoded for a type-HC RING-finger/putative E3 ligase protein which we termed NtRING1. The expression of NtRING1 was upregulated upon HR induction by elicitin, Ralstonia solanacearum, or tobacco mosaic virus (TMV) in tobacco. Silencing of NtRING1 remarkably delayed the establishment of elicitin-induced HR in tobacco as well as the expression of different early induction genes in tissues undergoing HR. Accordingly, transient overexpression of NtRING1 accelerated the HR launching upon elicitin treatment. Taking together, our data suggests that NtRING1 plays a functional role in the early establishment of HR.234.
Valérie Risson Laetitia Mazelin Mila Roceri Hervé Sanchez Vincent Moncollin Claudine Corneloup Hélène Richard-Bulteau Alban Vignaud Dominique Baas Aurélia Defour Damien Freyssenet Jean-Fran?ois Tanti Yannick Le-Marchand-Brustel Bernard Ferrier Agnès Conjard-Duplany Klaas Romanino Stéphanie Bauché Daniel Hanta? Matthias Mueller Sara C. Kozma George Thomas Markus A. Rüegg Arnaud Ferry Mario Pende Xavier Bigard Nathalie Koulmann Laurent Schaeffer Yann-Ga?l Gangloff 《The Journal of cell biology》2009,187(6):859-874
235.
Aurelie Chantome Alban Girault Marie Potier Christine Collin Pascal Vaudin Jean-Christophe Pagès Christophe Vandier Virginie Joulin 《Experimental cell research》2009,315(20):3620-3630
Cell migration and invasion are required for tumour cells to spread from the primary tumour bed so as to form secondary tumours at distant sites. We report evidence of an unusual expression of KCa2.3 (SK3) protein in melanoma cell lines but not in normal melanocytes. Knockdown of the KCa2.3 channel led to plasma membrane depolarization, decreased 2D and 3D cell motility. Conversely, enforced production of KCa2.3 protein in KCa2.3 non-expressing cells led to the plasma membrane becoming hyperpolarized, and enhanced cell motility. In contrast, KCa3.1 channels had no effect on cell motility despite an active role in regulating membrane potential. Our data also suggest that membrane hyperpolarization increases melanoma cell motility and that this occurs through the KCa2.3 channel. Our findings reveal a previously unknown function of the KCa2.3 channel, and suggest that the KCa2.3 channel might be the only member of the Ca2+-activated K+ channel family involved in melanoma cell motility pathways. 相似文献
236.
Alban Gervais Jean-Christophe Eymard Eric Toulmonde Jacky Bernard 《Cancer immunology, immunotherapy : CII》2009,58(11):1831-1841
Background Alloreaction is known to accumulate several theoretical advantages that can improve dendritic cell (DC)-based anti-infective
or antitumour strategies. Allogeneic DC have already been tested in experimental and clinical studies, but their efficacy
compared with their autologous counterparts was rarely investigated and conclusions diverge.
Objective This study compared antigen-specific T cell responses following priming with autologous versus allogeneic DC and examined
the possibility of screening these responses in order to select allogeneic DC that lead to a great amplification.
Results Allogeneic DC obtained from donors matched with the single HLA-A2 allele were efficient in generating in vitro peptide-specific
T cell responses. When randomly chosen, allogeneic DC generated a broad range of antigen-specific T cell responses in comparison
with autologous DC. When screened and selected, allogeneic DC markedly enhanced peptide-specific T cell priming and allowed
a more efficient boosting of resulting T cells. These selected allogeneic DC provided a favourable cytokinic and cellular
environment that can help concurrent antigen-specific responses.
Conclusion Ex vivo selected allogeneic DC provide adjuvant effects that lead to amplification of concomitant antigen-specific T cell
responses.
A. Gervais and J.-C. Eymard contributed equally to this work. 相似文献
237.
This study examined variations of social call structures in female Campbell's monkeys. The comparison of frequency modulations using a similarity index revealed that each individual presented one to three variants. Some variants were shared by two to several individuals, often associated with play. On the contrary, vocal divergence was observed in a socially isolated animal and a negative correlation appeared between similarity index and the frequency of avoidance. This preliminary study gives the first evidence of variant sharing between group members in primates. 相似文献
238.
Reactions of MDA with primary amino groups produce inter- or intra-molecular 1-amino-3-imino-propene (AIP) bridges, leading to structural modifications of biological molecules. In this work, applying electrophoresis followed by transfer onto nitrocellulose membranes, we observed that serum of a rabbit immunized with MDA-modified lysozyme (ML) reacts not only with ML and native lysozyme (L), but also with MDA-modified ribonuclease, cytochrome c or polylysine (MR, MC and MP respectively), while it does not react with native ribonuclease, cytochrome c or polylysine (R, C and P respectively). These results confirm previous ones indicating that sera of rabbits immunized with ML contain antibodies reacting specifically with epitopes containing AIP bridges. 相似文献
239.
Jördis F. Terlau Ulrich Brose Nico Eisenhauer Angelos Amyntas Thomas Boy Alexander Dyer Alban Gebler Christian Hof Tao Liu Christoph Scherber Ulrike E. Schlägel Anja Schmidt Myriam R. Hirt 《Global Change Biology》2023,29(13):3747-3758
Anthropogenic global warming has major implications for mobile terrestrial insects, including long-term effects from constant warming, for example, on species distribution patterns, and short-term effects from heat extremes that induce immediate physiological responses. To cope with heat extremes, they either have to reduce their activity or move to preferable microhabitats. The availability of favorable microhabitat conditions is strongly promoted by the spatial heterogeneity of habitats, which is often reduced by anthropogenic land transformation. Thus, it is decisive to understand the combined effects of these global change drivers on insect activity. Here, we assessed the movement activity of six insect species (from three orders) in response to heat stress using a unique tracking approach via radio frequency identification. We tracked 465 individuals at the iDiv Ecotron across a temperature gradient up to 38.7°C. In addition, we varied microhabitat conditions by adding leaf litter from four different tree species to the experimental units, either spatially separated or well mixed. Our results show opposing effects of heat extremes on insect activity depending on the microhabitat conditions. The insect community significantly decreased its activity in the mixed litter scenario, while we found a strong positive effect on activity in the separated litter scenario. We hypothesize that the simultaneous availability of thermal refugia as well as resources provided by the mixed litter scenario allows animals to reduce their activity and save energy in response to heat stress. Contrary, the spatial separation of beneficial microclimatic conditions and resources forces animals to increase their activity to fulfill their energetic needs. Thus, our study highlights the importance of habitat heterogeneity on smaller scales, because it may buffer the consequences of extreme temperatures of insect performance and survival under global change. 相似文献
240.
Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Protein biotinylation is an original and very specific posttranslational modification, compartmented in plants, between mitochondria, plastids, and the cytosol. This reaction modifies and activates few carboxylases committed in key metabolisms and is catalyzed by holocarboxylase synthetase (HCS). The molecular bases of this complex compartmentalization and the relative function of each of the HCS genes, HCS1 and HCS2, identified in Arabidopsis (Arabidopsis thaliana) are mainly unknown. Here, we showed by reverse genetics that the HCS1 gene is essential for plant viability, whereas disruption of the HCS2 gene in Arabidopsis does not lead to any obvious phenotype when plants are grown under standard conditions. These findings strongly suggest that HCS1 is the only protein responsible for HCS activity in Arabidopsis cells, including the cytosolic, mitochondrial, and plastidial compartments. A closer study of HCS1 gene expression enabled us to propose an original mechanism to account for this multiplicity of localizations. Located in the HCS1 messenger RNA 5'-untranslated region, an upstream open reading frame regulates the translation initiation of HCS1 and the subsequent targeting of HCS1 protein. Moreover, an exquisitely precise alternative splicing of HCS1 messenger RNA can regulate the presence and absence of this upstream open reading frame. The existence of these complex and interdependent mechanisms creates a rich molecular platform where different parameters and factors could control HCS targeting and hence biotin metabolism. 相似文献