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61.
Fermentation of barley straw by anaerobic rumen bacteria and fungi in axenic culture and in co-culture with methanogens 总被引:2,自引:2,他引:0
K.N. Joblin Gillian P. Campbell A.J. Richardson C.S. Stewart † 《Letters in applied microbiology》1989,9(5):195-197
When incubated in axenic culture, strains of anaerobic rumen fungi were more active than cellulolytic bacteria in solubilizing barley straw stem fragments 5 to 10 mm in length. Pretreatment with ammonia had little effect on microbial attack. Of three species of methanogens tested, Methanobrevibacter smithii strain PS formed the most stable and reproducible co-cultures with the fungi and with Ruminococcus albus , and the presence of this organism enhanced the extent of degradation of straw, although this effect was less marked than that previously observed when pure cellulose was used as substrate. 相似文献
62.
Identification of a cysteine residue at the active site of Escherichia coli isocitrate lyase. 总被引:3,自引:1,他引:2
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H G Nimmo F Douglas C Kleanthous D G Campbell C MacKintosh 《The Biochemical journal》1989,261(2):431-435
Escherichia coli isocitrate lyase was inactivated by iodacetate in a pseudo-first-order process. Complete inactivation was associated with the incorporation of only one carboxymethyl group per enzyme subunit. The substrate and products of the enzyme protected against inactivation, suggesting that the reactive group may be located at the active site. Isolation and sequencing of a carboxymethylated peptide showed that the modified residue was a cysteine, in the sequence Cys-Gly-His-Met-Gly-Gly-Lys. The reactivity of isocitrate lyase to iodoacetate declined with pH, following a titration curve for a group of pKa 7.1. The Km of the enzyme for isocritrate declined over the same pH range. 相似文献
63.
Identification of a novel metabolite in phenanthrene metabolism by the fungus Cunninghamella elegans
C E Cerniglia W L Campbell J P Freeman F E Evans 《Applied and environmental microbiology》1989,55(9):2275-2279
The metabolism of phenanthrene by the fungus Cunninghamella elegans was investigated. Kinetic experiments using [9-14C]phenanthrene showed that after 72 h, 53% of the total radioactivity was associated with a glucoside conjugate of 1-hydroxyphenanthrene (phenanthrene 1-O-beta-glucose). This metabolite was isolated by reversed-phase high-performance liquid chromatography and characterized by the application of UV absorption, 1H nuclear magnetic resonance, and mass spectral techniques. The results show that aromatic ring oxidation followed by glucosylation is a predominant pathway in the metabolism of the polycyclic aromatic hydrocarbon phenanthrene by C. elegans. 相似文献
64.
A framework is presented for interpreting frequency distributions of volume or fluorescence as measured by a flow cytometer on homogeneous phytoplankton populations. The framework, based on both laboratory experience and theoretical concepts, is illustrated with the use of a simulation model. Asynchronous, synchronous, and phased populations were simulated, with constant and variable growth patterns over the cell cycle. Though simulations produced a wide variety of histogram shapes, including multimodal distributions, the primary difference between asynchronous and synchronous/phased distributions lies in their temporal variation. Histograms that are constant in time indicate asynchronous populations; when populations are not asynchronous, their histogram shapes vary with a periodicity on the same time scale as the cell cycle. A probability density function for the case of asynchronous populations with a constant growth rate is derived. When fitted to simulated histograms this two-parameter density function yields estimates of the two parameters: mean and variance of cell volume (or mass) at age 0. 相似文献
65.
66.
Phylogenetic studies suggest that mobile element families are unstable components of the Drosophila genome. Two examples of immobilization of a transposable element family are presented here: as judged by their constant genomic organization among unrelated strains, the F and I element families have been respectively immobilized for a long time in D. simulans and in the reactive D. melanogaster strains (these are the laboratory strains which escaped the recent I invasion of D. melanogaster natural populations). All the elements of these defective families are located in the heterochromatic portion of the genome. Moreover, most if not all of the heterochromatic sequences into which the defective I elements are embedded are themselves non-mobile members of various nomadic families such as mdg 4, 297, 1731, F and Doc. These results are discussed with special emphasis on the possible nomadic origin of heterochromatin components and on the mechanisms of evolutionary turnover of the transposable element families. 相似文献
67.
Phosphorylation of bovine hormone-sensitive lipase by the AMP-activated protein kinase. A possible antilipolytic mechanism 总被引:15,自引:0,他引:15
A J Garton D G Campbell D Carling D G Hardie R J Colbran S J Yeaman 《European journal of biochemistry》1989,179(1):249-254
Hormone-sensitive lipase is phosphorylated at a single site (site 2) in vitro by the AMP-activated protein kinase, without any direct effect on the activity of the enzyme. The amino acid sequence around this site has been determined. Ca2+/calmodulin-dependent protein kinase II also phosphorylates hormone-sensitive lipase predominantly at this site, whilst cyclic-GMP-dependent protein kinase phosphorylates exclusively the regulatory site (site 1) which is also phosphorylated by cyclic-AMP-dependent protein kinase. Phosphorylation of site 2 has been found to inhibit subsequent phosphorylation and activation of hormone-sensitive lipase by the cyclic-AMP-dependent and cyclic-GMP-dependent protein kinases, indicating that site-2 phosphorylation may have an antilipolytic role in vivo. 相似文献
68.
Translational regulation of ferritin synthesis in rat spleen: effects of iron and inflammation 总被引:2,自引:0,他引:2
C H Campbell R M Solgonick M C Linder 《Biochemical and biophysical research communications》1989,160(2):453-459
Translational control of ferritin synthesis was studied in rat spleen, and compared with that for liver, heart and brain, in response to iron and inflammation. Spleen concentrations of total RNA in the ribonucleoprotein (mRNP) fraction was comparable to that for liver, while polyribosomal RNA was less. Both fractions were ten-fold lower in heart and brain. In untreated animals, the mRNP fraction of all tissues had the largest portion of the ferritin mRNA, as determined by slot blot hybridization with 32P-labeled cDNA for the L subunit. Acute treatment with ferric ammonium citrate shifted the spleen ferritin mRNA to the polyribosome fraction. This was also so in liver but not in the heart and brain which took up much less iron. The findings were confirmed by hybridization studies of mRNPs and polyribosomes separated in sucrose gradients. Turpentine-induced inflammation also caused a shift in ferritin mRNA from the mRNP to the polyribosome fraction of spleen and liver, over 12 h. We conclude that as in liver, spleen ferritin synthesis is under translational control by iron, and that both tissues also respond to inflammation by shifting of ferritin mRNA to the polyribosomes. 相似文献
69.
P K Paul P Dauber-Osguthorpe M M Campbell D J Osguthorpe 《Biochemical and biophysical research communications》1989,165(3):1051-1058
A 50 pico-second molecular dynamics simulation on a cyclic LHRH antagonist analogue Ac-D-Phe1-D-Phe2-D-Trp3-Ser4-Glu5-D-Arg6-Leu7-Lys8+ ++-Pro9-D-Ala10-NH2 (where the cyclisation is via an amide linkage between the Glu5 and Lys8 side chains), reveals some hitherto unseen conformational features. The LHRH analogue is found to adopt a near beta-sheet type of conformation with the reversal in the chain being brought about by a D-Trp3-Ser4-Glu5-D-Arg6 beta turn. The N- and C-terminal ends of the peptide come close together and interact through a network of hydrogen bonds. Additional hydrogen bonds expected of a sheet type of conformation stabilise the lowest energy minima. A conformational search of all possible cyclic structures of a model system c(Glu-D-Ala-Ala-Lys) which was used to determine the starting structure for the simulation studies of the cyclic LHRH antagonist analogue is also highlighted. The influence of the cyclic part on the conformation of this LHRH analogue is discussed. 相似文献
70.
An unstable carboxypeptidase N or B like enzyme is generated as a result of coagulation. This enzyme is derived from some plasma component (s) and not from blood cells or platelets. Furthermore, the activity generated is specific for arginine substrates insofar as small synthetic substrates are concerned. The enzyme is unrelated to CPN or any of its subunits or subunit fragments. This transient carboxypeptidase may be involved in the processing and/or scheduling of different functions of bioactive peptides generated during inflammation. 相似文献