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排序方式: 共有146条查询结果,搜索用时 15 毫秒
111.
Daniel Silqueira Martins Guimar?es Amanda Luisa da Fonseca Ronan Batista Moacyr Comar Junior Alaíde Braga de Oliveira Alex Gutterres Taranto Fernando de Pilla Varotti 《Memórias do Instituto Oswaldo Cruz》2015,110(2):255-258
Malaria is responsible for more deaths around the world than any other parasitic
disease. Due to the emergence of strains that are resistant to the current
chemotherapeutic antimalarial arsenal, the search for new antimalarial drugs remains
urgent though hampered by a lack of knowledge regarding the molecular mechanisms of
artemisinin resistance. Semisynthetic compounds derived from diterpenes from the
medicinal plant Wedelia paludosa were tested in silico against
the Plasmodium falciparum Ca2+-ATPase, PfATP6. This
protein was constructed by comparative modelling using the three-dimensional
structure of a homologous protein, 1IWO, as a scaffold. Compound 21 showed the best
docking scores, indicating a better interaction with PfATP6 than that of
thapsigargin, the natural inhibitor. Inhibition of PfATP6 by diterpene compounds
could promote a change in calcium homeostasis, leading to parasite death. These data
suggest PfATP6 as a potential target for the antimalarial
ent-kaurane diterpenes. 相似文献
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Denis Belitkin Shishir M Pant Pauliina Munne Ilida Suleymanova Kati Belitkina HannaAla Hongisto Johanna Englund Tiina Raatikainen Olga Klezovitch Valeri Vasioukhin Shuo Li Qingyu Wu Outi Monni Satu Kuure Pirjo Laakkonen Jeroen Pouwels Topi A Tervonen Juha Klefstrm 《EMBO reports》2021,22(11)
Transforming growth factor‐beta (TGFβ) is a multifunctional cytokine with a well‐established role in mammary gland development and both oncogenic and tumor‐suppressive functions. The extracellular matrix (ECM) indirectly regulates TGFβ activity by acting as a storage compartment of latent‐TGFβ, but how TGFβ is released from the ECM via proteolytic mechanisms remains largely unknown. In this study, we demonstrate that hepsin, a type II transmembrane protease overexpressed in 70% of breast tumors, promotes canonical TGFβ signaling through the release of latent‐TGFβ from the ECM storage compartment. Mammary glands in hepsin CRISPR knockout mice showed reduced TGFβ signaling and increased epithelial branching, accompanied by increased levels of fibronectin and latent‐TGFβ1, while overexpression of hepsin in mammary tumors increased TGFβ signaling. Cell‐free and cell‐based experiments showed that hepsin is capable of direct proteolytic cleavage of fibronectin but not latent‐TGFβ and, importantly, that the ability of hepsin to activate TGFβ signaling is dependent on fibronectin. Altogether, this study demonstrates a role for hepsin as a regulator of the TGFβ pathway in the mammary gland via a novel mechanism involving proteolytic downmodulation of fibronectin. 相似文献
115.
Bi-Functional Biobased Packing of the Cassava Starch,Glycerol, Licuri Nanocellulose and Red Propolis
Samantha Serra Costa Janice Izabel Druzian Bruna Aparecida Souza Machado Carolina Oliveira de Souza Alaíse Gil Guimar?es 《PloS one》2014,9(11)
The aim of this study was to characterize and determine the bi-functional efficacy of active packaging films produced with starch (4%) and glycerol (1.0%), reinforced with cellulose nanocrystals (0–1%) and activated with alcoholic extracts of red propolis (0.4 to 1.0%). The cellulose nanocrystals used in this study were extracted from licuri leaves. The films were characterized using moisture, water-activity analyses and water vapor-permeability tests and were tested regarding their total phenolic compounds and mechanical properties. The antimicrobial and antioxidant efficacy of the films were evaluated by monitoring the use of the active films for packaging cheese curds and butter, respectively. The cellulose nanocrystals increased the mechanical strength of the films and reduced the water permeability and water activity. The active film had an antimicrobial effect on coagulase-positive staphylococci in cheese curds and reduced the oxidation of butter during storage. 相似文献
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Nicolas Sauvageot Sonia Leite Ala’a Alkerwi Leila Sisanni Faiez Zannad Stranges Saverio Anne-Fran?oise Donneau Adelin Albert Michèle Guillaume 《PloS one》2016,11(8)
IntroductionPrincipal component analysis is used to determine dietary behaviors of a population whereas reduced rank regression is used to construct disease-related dietary patterns. This study aimed to compare both types of DP and theirs associations with cardiovascular risk factors (CVRF).ResultsWe found that CVRF-related patterns also reflect eating behaviours of the population. Comparing concordant food groups between both dietary pattern methods, a diet high in fruits, oleaginous and dried fruits, vegetables, olive oil, fats rich in omega 6 and tea and low in fried foods, lean and fatty meat, processed meat, ready meal, soft drink and beer was associated with lower prevalence of CVRF. In the opposite, a pattern characterized by high intakes of fried foods, meat, offal, beer, wine and aperitifs and spirits, and low intakes of cereals, sugar and sweets and soft drinks was associated with higher prevalence of CVRF.ConclusionIn sum, we found that a “Prudent” and “Animal protein and alcohol” patterns were both associated with CVRF and behaviourally meaningful. Moreover, the relationships of those dietary patterns with lifestyle characteristics support the theory that food choices are part of a larger pattern of healthy lifestyle. 相似文献
118.
We examined the apoptotic and anti-proliferative effects of all-trans retinoic acid (atRA) in HeLa cells. Our results demonstrated that HeLa cells were more sensitive to the anti-proliferative effects of atRA than to its apoptotic effects. Furthermore, we demonstrated that caspase inhibition attenuates cell death but does not alter the atRA-dependent reduction in cell proliferation, which suggests that atRA-induced apoptosis is independent of the arrest in cell proliferation. To check whether ANT proteins mediated these atRA effects, we transiently transfected cells with expression vectors encoding for individual ANT (adenine nucleotide translocase 1-3). Our results revealed that ANT1 and ANT3 over-expressing HeLa cells increased their atRA sensitivity. Thus, our results not only demonstrate the different functional activities of ANT isoforms, but also contribute to a better understanding of the properties of atRA as an anti-tumoral agent used in cancer therapy. 相似文献
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