全文获取类型
收费全文 | 1376篇 |
免费 | 51篇 |
国内免费 | 2篇 |
专业分类
1429篇 |
出版年
2024年 | 3篇 |
2023年 | 24篇 |
2022年 | 32篇 |
2021年 | 59篇 |
2020年 | 36篇 |
2019年 | 54篇 |
2018年 | 47篇 |
2017年 | 40篇 |
2016年 | 66篇 |
2015年 | 96篇 |
2014年 | 78篇 |
2013年 | 113篇 |
2012年 | 107篇 |
2011年 | 94篇 |
2010年 | 68篇 |
2009年 | 45篇 |
2008年 | 56篇 |
2007年 | 52篇 |
2006年 | 46篇 |
2005年 | 38篇 |
2004年 | 40篇 |
2003年 | 36篇 |
2002年 | 32篇 |
2001年 | 12篇 |
2000年 | 14篇 |
1999年 | 13篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1987年 | 4篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1979年 | 3篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1969年 | 7篇 |
1965年 | 3篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1957年 | 2篇 |
排序方式: 共有1429条查询结果,搜索用时 0 毫秒
11.
Gerhard H. Schmidt Kamal S. A. Othman 《Archives of insect biochemistry and physiology》1993,24(1):45-54
In Aiolopus thalassinus (Fabr.), the changes in the volume of the corpora allata (CA), in the concentration of juvenile hormone III (JH III) in the hemolymph, and in protein content in the terminal (t) oocytes were studied during the first gonadotropic cycle. These parameters could be better related to the volume of t-oocytes than to age after emergence. The JH III titer curve was maximum (2.9 pmol/10 μl) at an oocyte volume of 1.2 mm3. Before oviposition (days 10–16) the JH III titer decreased to 0.65 pmol/10 μl hemolyph. The increase in JH III titer reflected a period of high protein storage in the t-oocytes. The largest volume of the CA was reached at the beginning of yolk storage in the t-oocytes. The highest JH III titer did not correspond with the largest volume of CA, which occurred much earlier. © 1993 Wiley-Liss, Inc. 相似文献
12.
Shafiq Fahad Iqbal Muhammad Raza Syed Hammad Akram Nudrat Aisha Ashraf Muhammad 《Journal of Plant Growth Regulation》2023,42(3):1267-1290
Journal of Plant Growth Regulation - Fullerenols are carbon nanoparticles that have been declared as free radical sponges. There is a need to take a prudent path toward its applications in various... 相似文献
13.
The cat is the primary model for neuromuscular research. However, sarcomere geometry, in particular thin-myofilament lengths of cat skeletal muscles, is not known, thus preventing adequate muscle modeling on the sarcomere level. The purpose of this study was to determine thin-myofilament lengths in cat skeletal muscle. It was found that average thin-myofilament lengths of cat tibialis anterior muscles (1.12 microns) were larger than the average values reported for frog (approximately 0.95 microns), rat (1.09 microns), and rabbit muscles (1.09 microns) and were smaller than the values reported for monkey (1.16 microns) and human skeletal muscles (1.27 microns). According to the cross-bridge theory of muscular contraction, this result implies that the range of sarcomere length on the ascending limb of the force-length relation for cat muscle is between those of frog, rat, and rabbit on the one side and monkey and human on the other side. It is speculated that the differences in thin-myofilament lengths of different animals are related to the functional demands of these muscles in everyday movement tasks. Isolated experimental observations appear to support this speculation. 相似文献
14.
Chemically different classes of calcium channel blockers were examined in rats for their effects on behavior, tolerability and protection against maximal electroshock seizures (MES) and pentylenetetrazol (PTZ) induced seizures. In MES test at doses (mg/kg, ip) that were devoid of side effects, felodipine, 50, afforded 100% protection, while nimodipine, 5; pimozide, 10; and thioridazine, 25, showed 50 to 66% protection. Nifedipine, 10, and diltiazem, 50, showed 30 and 66% protection respectively, but were associated with side effects. Verapamil and loperamide were ineffective against MES and PTZ induced seizures. Nimodipine, 1 mg/kg, ip, was the most potent agent and produced 100% protection against PTZ. Equieffective doses were pimozide, 25, felodipine, 50, and thioridazine, 50. The rest of the calcium channel blockers showed marginal to moderate activity against chemoshock. The data obtained suggest that some calcium channel blockers possess anticonvulsant activity and may be considered as adjuvant therapeutic agents in epileptics refractory to conventional antiepileptic medication. 相似文献
15.
We have analyzed, by means of density functional theory calculations and the embedded cluster model, the adsorption and spin-state
properties of Cr, Ni, Mo, and Pt deposited on a MgO crystal. We considered deposition at the Mg2+ site of a defect-free surface and at Li+ and Na+ sites of impurity-containing surfaces. To avoid artificial polarization effects, clusters of moderate sizes with no border
anions were embedded in simulated Coulomb fields that closely approximate the Madelung fields of the host surfaces. The interaction
between a transition metal atom and a surface results from a competition between Hund's rule for the adsorbed atom and the
formation of a chemical bond at the interface. We found that the adsorption energies of the metal atoms are significantly
enhanced by the cation impurities, and the adsorption energies of the low-spin states of spin-quenched complexes are always
more favorable than those of the high-spin states. Spin polarization effects tend to preserve the spin states of the adsorbed
atoms relative to those of the isolated atoms. The metal–support interactions stabilize the low-spin states of the adsorbed
metals with respect to the isolated metals, but the effect is not always enough to quench the spin. Spin quenching occurs
for Cr and Mo complexes at the Mg2+ site of the pure surface and at Li+ and Na+ sites of the impurity-containing surfaces. Variations of the spin-state properties of free metals and of the adsorption and
spin-state properties of metal complexes are correlated with the energies of the frontier orbitals. The electrostatic potential
energy curves provide further understanding of the nature of the examined properties. 相似文献
16.
Nageshwar YV Sheelu G Shambhu RR Muluka H Mehdi N Malik MS Kamal A 《Bioprocess and biosystems engineering》2011,34(5):515-523
Microbial nitrilases are biocatalysts of interest and the enzyme produced using various inducers exhibits altered substrate
specificity, which is of great interest in bioprocess development. The aim of the present study is to investigate the nitrilase-producing
Alcaligenes faecalis MTCC 10757 (IICT-A3) for its ability to transform various nitriles in the presence of different inducers after optimization
of various parameters for maximum enzyme production and activity. The production of A. faecalis MTCC 10757 (IICT-A3) nitrilase was optimum with glucose (1.0%), acrylonitrile (0.1%) at pH 7.0. The nitrilase activity of
A. faecalis MTCC 10757 (IICT-A3) was optimum at 35 °C, pH 8.0 and the enzyme was stable up to 6 h at 50 °C. The nitrilase enzyme produced
using different inducers was investigated for substrate specificity. The enzyme hydrolyzed aliphatic, heterocyclic and aromatic
nitriles with different substitutions. Acrylonitrile was the most preferred substrate (~40 U) as well as inducer. Benzonitrile
was hydrolyzed with almost twofold higher relative activity than acrylonitrile when it was used as an inducer. The versatile
nitrilase-producing A. faecalis MTCC 10757 (IICT-A3) exhibits efficient conversion of both aliphatic and aromatic nitriles. The aromatic nitriles, which
show not much or no affinity towards nitrilase from A. faecalis, are hydrolyzed effectively with this nitrilase-producing organism. Studies are in progress to exploit this organism for
synthesis of industrially important compounds. 相似文献
17.
This study was carried out on the transported bed soil dredged from the outlet of Lake Burullus to the Mediterranean Sea and deposited nearby, forming by this way new land that underwent a primary plant succession. The multi-methodological approach comprised floristic inventories, vegetation sampling and soil composition analyses of the study site in order to detect the crucial parameters controlling the plant resettlement on recently deposited soil as related to time, local micro-topography and substrate characteristics. Floristic composition was assessed for the first 10 years of primary succession (2001–2010) on 18 stands of the area, distributed on basement, slope stands and plateau of the landfill, respectively. Vegetation surveys were the basis of multivariate analyses of the vegetation and soil data using TWINSPAN, DCA and CCA. Relationships between the edaphic gradients, floristic composition and species diversity were assessed. 相似文献
18.
Kissner TL Moisan L Mann E Alam S Ruthel G Ulrich RG Rebek M Rebek J Saikh KU 《The Journal of biological chemistry》2011,286(36):31385-31396
19.
20.
Akram Mirzaei Sina Rashedi Mohammad Reza Akbari Fatemeh Khatami Seyed Mohammad Kazem Aghamir 《Journal of cellular and molecular medicine》2022,26(9):2728
Arsenic trioxide (ATO) and statins have been demonstrated to have anti‐neoplastic properties; however, the data regarding their combination therapy is limited. Thus, we aimed to study the effects of ATO, Simvastatin and their combination in proliferation, apoptosis and pathological angiogenesis in prostate cancer cell lines. The human prostate cell lines were treated with different concentrations of Simvastatin and ATO alone and combined to find effective doses and IC50 values. In addition, the percentage of apoptotic cells was evaluated by annexin/PI staining, and mRNA expression levels of the apoptotic gene, including OPN isoforms and VEGF, were investigated using real‐time PCR. Our data displayed that Simvastatin (12 and 8 μM in PC3 and LNCaP cell lines respectively), ATO (8 and 5 μM in PC3 and LNCaP cell lines respectively), and also their combination (12 μM Simvastatin and 8 μM ATO in PC3, 8 μM Simvastatin and 5 μM ATO in LNCaP cell lines respectively) significantly increased the percentage of apoptotic cells. Also, we showed that the combination therapy by Simvastatin and ATO increased cell apoptosis and inhibited cell proliferation, providing anti‐proliferative and anti‐angiogenic properties, possibly via downregulation of the expression of VEGF and OPN genes. These results provide new perceptions regarding the anticancer roles of ATO and statins’ combination therapy in prostate cancer. 相似文献