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51.
Rabbits were immunized with dinitrophenyl-coupled Ascaris antigen (DNP-Asc) or ragweed antigen (DNP-Rag) included in aluminum hydroxide gel and their mesenteric lymph node cells were cultured for 24 hr in vitro in the presence of free homologous carrier. The cell-free supernatant thus obtained enhanced both IgG and IgE antihapten antibody responses of DNP-primed cells to DNP-heterologous carrier conjugate (DNP-keyhole limpet hemocyanin). Since the cell-free supernatant obtained from Rag-specific cells enhanced antibody response of hapten-primed cells raised by immunization with DNP-Asc, no carrier specificity was involved in the enhancement. It was found that treatment of primed cells with 10-5 M pactamycin suppressed the formation of the enhancing soluble factor, whereas the factor was readily formed in the presence of 2 mug/mol of cytosine arabinoside in the culture. The results indicated that cell proliferation was not required but de novo synthesis of protein was essential for the formation of soluble factor(s). The enhancing factor was not absorbed by either carrier-coated or anti-carrier antibody-coated immunosorbent. It was also found that the enhancing factor was formed by incubating primed cells with carrier-coated Sepharose. The cell-free supernatant containing no free carrier enhanced both IgG and IgE anti-hapten antibody responses. The activities of the cell-free supernatant to enhance IgG and IgE antibody responses were not absorbed by anti-Fab, anti-gamma-or anti-mu-chain antibody immunosorbent, indicating that the nonspecific enhancing factor did not possess immunoglobulin determinant. The cell-free supernatant was fractionated by sucrose density gradient ultracentrifugation and by gel filtration with three radiolabeled proteins, i.e., IgG, ovalbumin, and cytochrome C as markers. Enhancing activity for IgG antibody response was recovered in a fraction between ovalbumin peak (40,000 m.w.) and cytochrome C peak (20,000 m.w.). The activity for IgE antibody response was recovered in a fraction containing IgG marker (150,000 m.w.). By block electrophoresis, both activities were detected in beta globulin fraction. The results suggested that different T cell factors are involved in the IgG and IgE antibody responses. 相似文献
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Ishikawa Junko Fujimura Shigeto Kondo Motohiko Murai-Hatano Mari Goto Akitoshi Shinano Takuro 《Plant and Soil》2018,424(1-2):503-524
Plant and Soil - In the Mediterranean basin, reduction in cloudiness owing to climate change is expected to enhance solar ultraviolet (UV) levels and to decrease rainfall over the coming years,... 相似文献
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Multiple centrosome formation induced by the expression of Vpr gene of human immunodeficiency virus.
Y Minemoto M Shimura Y Ishizaka Y Masamune K Yamashita 《Biochemical and biophysical research communications》1999,258(2):379-384
We previously established a cell line called MIT-23 in which expression of the Vpr gene of human immunodeficiency virus 1 (HIV-1) can be controlled by the addition of tetracycline. Vpr expression induces multiple nuclear formation and increased ploidy in MIT-23 cells. We herein report that multipolar mitotic spindles were formed upon induction of Vpr. Further analysis of centrosomes with anti-gamma-tubulin immunostaining revealed that a significant population of cells 1 week after expression of Vpr gene product had an increased number of centrosomes in the cells with abnormal nuclei. Taking into account that the centrosome plays an important role in genome integrity, the abnormal number of centrosomes in cells expressing Vpr may be directly related to aneuploidy or the formation of micronuclei in MIT-23 cells, suggesting that Vpr has an oncogenic role in HIV infected cells. 相似文献
58.
Yoshikawa M Nishiofuku M Moriya K Ouji Y Ishizaka S Kasahara K Mikasa K Hirai T Mizuno Y Ogawa S Nakamura T Maruyama H Akao N 《Parasitology international》2008,57(4):525-529
We present 3 adult cases of visceral toxocariasis from the same family, who each consumed thin slices of raw bovine liver weekly, and developed eosinophilia and multiple small lesions in their livers and lungs. Serological examinations using the larval excretory–secretory product of Toxocara canis strongly indicated infection with Toxocara species larvae. The patients responded well to treatment with albendazole. Ingestion of raw liver from paratenic animals is considered to be a common transmission route of human toxocariasis, especially in adults. 相似文献
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Hiroki Matsuyama Fumimasa Amaya Soshi Hashimoto Hiroshi Ueno Satoru Beppu Mitsuhiko Mizuta Nobuaki Shime Akitoshi Ishizaka Satoru Hashimoto 《Respiratory research》2008,9(1):1-13
Background
Many studies associated the main polyphenolic constituent of green tea, (-)-Epigallocatechin-3-gallate (EGCG), with inhibition of cancers, invasion and metastasis. To date, most of the studies have focused on the effect of EGCG on cell proliferation or death. Since cell migration is an important mechanism involved in tumor invasion, the aim of the present work was to target another approach of the therapeutic effect of EGCG, by investigating its effect on the cell migratory behavior.Methods
The effect of EGCG (at concentrations lower than 10 μg/ml) on the migration speed of invasive cells was assessed by using 2D and 3D models of cell culture. We also studied the effects of EGCG on proteinases expression by RT-PCR analysis. By immunocytochemistry, we analyzed alterations of vimentin organization in presence of different concentrations of EGCG.Results
We observed that EGCG had an inhibitory effect of cell migration in 2D and 3D cell culture models. EGCG also inhibited MMP-2 mRNA and protein expression and altered the intermediate filaments of vimentin.Conclusion
Taken together, our results demonstrate that EGCG is able to inhibit the migration of bronchial tumor cells and could therefore be an attractive candidate to treat tumor invasion and cell migration. 相似文献60.
Tatsuhisa Takahashi Taiji Nagaoka Hirotaka Yanagida Tadashi Saitoh Akira Kamiya Travis Hein Lih Kuo Akitoshi Yoshida 《Journal of Biorheology》2009,23(2):77-86
To quantitatively assess the arteriovenous distribution of hemodynamic parameters throughout the microvascular network of
the human retina, we constructed a retinal microcirculatory model consisting of a dichotomous symmetric branching system.
This system is characterized by a diameter exponent of 2.85, instead of 3 as dictated by Murray’s law, except for the capillary
networks. The value of 2.85 was the sum of a fractal dimension (1.70) and a branch exponent (1.15) of the retinal vasculature.
Following the feeding artery (central retinal artery), each bifurcation was recursively developed at a distance of an individual
branch length [L(r) = 7.4r
1.15] by a centrifugal scheme. The venular tree was formed in the same way. Using this model, we evaluated hemodynamic parameters,
including blood pressure, blood flow, blood velocity, shear rate, and shear stress, within the retinal microcirculatory network
as a function of vessel diameter. The arteriovenous distributions of blood pressure and velocity in the simulation were consistent
with in vivo measurements in the human retina and other vascular beds of small animals. We therefore conclude that the current
theoretical model was useful for quantifying hemodynamics as a function of vessel diameter within the retinal microvascular
network. 相似文献