全文获取类型
收费全文 | 5235篇 |
免费 | 328篇 |
国内免费 | 2篇 |
专业分类
5565篇 |
出版年
2022年 | 23篇 |
2021年 | 58篇 |
2020年 | 36篇 |
2019年 | 52篇 |
2018年 | 53篇 |
2017年 | 54篇 |
2016年 | 82篇 |
2015年 | 167篇 |
2014年 | 174篇 |
2013年 | 260篇 |
2012年 | 325篇 |
2011年 | 299篇 |
2010年 | 184篇 |
2009年 | 201篇 |
2008年 | 312篇 |
2007年 | 323篇 |
2006年 | 305篇 |
2005年 | 333篇 |
2004年 | 321篇 |
2003年 | 294篇 |
2002年 | 269篇 |
2001年 | 141篇 |
2000年 | 132篇 |
1999年 | 122篇 |
1998年 | 58篇 |
1997年 | 53篇 |
1996年 | 39篇 |
1995年 | 42篇 |
1994年 | 38篇 |
1993年 | 38篇 |
1992年 | 97篇 |
1991年 | 69篇 |
1990年 | 51篇 |
1989年 | 63篇 |
1988年 | 57篇 |
1987年 | 54篇 |
1986年 | 42篇 |
1985年 | 47篇 |
1984年 | 32篇 |
1983年 | 29篇 |
1982年 | 25篇 |
1981年 | 25篇 |
1980年 | 19篇 |
1979年 | 25篇 |
1978年 | 17篇 |
1977年 | 15篇 |
1976年 | 13篇 |
1974年 | 11篇 |
1970年 | 10篇 |
1969年 | 13篇 |
排序方式: 共有5565条查询结果,搜索用时 15 毫秒
991.
Masaaki Umeda Momoko Ikeuchi Masaki Ishikawa Toshiro Ito Ryuichi Nishihama Junko Kyozuka Keiko U. Torii Akiko Satake Gohta Goshima Hitoshi Sakakibara 《The Plant journal : for cell and molecular biology》2021,106(2):326-335
Plant stem cells have several extraordinary features: they are generated de novo during development and regeneration, maintain their pluripotency, and produce another stem cell niche in an orderly manner. This enables plants to survive for an extended period and to continuously make new organs, representing a clear difference in their developmental program from animals. To uncover regulatory principles governing plant stem cell characteristics, our research project ‘Principles of pluripotent stem cells underlying plant vitality’ was launched in 2017, supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Japanese government. Through a collaboration involving 28 research groups, we aim to identify key factors that trigger epigenetic reprogramming and global changes in gene networks, and thereby contribute to stem cell generation. Pluripotent stem cells in the shoot apical meristem are controlled by cytokinin and auxin, which also play a crucial role in terminating stem cell activity in the floral meristem; therefore, we are focusing on biosynthesis, metabolism, transport, perception, and signaling of these hormones. Besides, we are uncovering the mechanisms of asymmetric cell division and of stem cell death and replenishment under DNA stress, which will illuminate plant-specific features in preserving stemness. Our technology support groups expand single-cell omics to describe stem cell behavior in a spatiotemporal context, and provide correlative light and electron microscopic technology to enable live imaging of cell and subcellular dynamics at high spatiotemporal resolution. In this perspective, we discuss future directions of our ongoing projects and related research fields. 相似文献
992.
A novel cloverleaf structure found in mammalian mitochondrial tRNA(Ser) (UCN). 总被引:3,自引:3,他引:3 下载免费PDF全文
T Yokogawa Y Watanabe Y Kumazawa T Ueda I Hirao K Miura K Watanabe 《Nucleic acids research》1991,19(22):6101-6105
Bovine mitochondrial tRNA(Ser) (UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been confirmed at the RNA level. In the course of investigating the structure and function of mitochondrial tRNAs, we have isolated the bovine liver mitochondrial tRNA(Ser) (UCN) and determined its complete sequence including the modified nucleotides. Analysis of the 5'-terminal nucleotide and enzymatic determination of the whole sequence of tRNA(Ser) (UCN) revealed that the tRNA started from the third nucleotide of the putative tRNA(Ser) (UCN) gene, which had formerly been supposed. Enzymatic probing of tRNA(Ser) (UCN) suggests that the tRNA possesses an unusual cloverleaf structure with the following characteristics. (1) There exists only one nucleotide between the acceptor stem with 7 base pairs and the D stem with 4 base pairs. (2) The anticodon stem seems to consist of 6 base pairs. Since the same type of cloverleaf structure as above could be constructed only for mitochondrial tRNA(Ser) (UCN) genes of mammals such as human, rat and mouse, but not for those of non-mammals such as chicken and frog, this unusual secondary structure seems to be conserved only in mammalian mitochondria. 相似文献
993.
Phytoplasma effector SAP54 induces indeterminate leaf-like flower development in Arabidopsis plants 总被引:2,自引:0,他引:2
MacLean AM Sugio A Makarova OV Findlay KC Grieve VM Tóth R Nicolaisen M Hogenhout SA 《Plant physiology》2011,157(2):831-841
Phytoplasmas are insect-transmitted bacterial plant pathogens that cause considerable damage to a diverse range of agricultural crops globally. Symptoms induced in infected plants suggest that these phytopathogens may modulate developmental processes within the plant host. We report herein that Aster Yellows phytoplasma strain Witches' Broom (AY-WB) readily infects the model plant Arabidopsis (Arabidopsis thaliana) ecotype Columbia, inducing symptoms that are characteristic of phytoplasma infection, such as the production of green leaf-like flowers (virescence and phyllody) and increased formation of stems and branches (witches' broom). We found that the majority of genes encoding secreted AY-WB proteins (SAPs), which are candidate effector proteins, are expressed in Arabidopsis and the AY-WB insect vector Macrosteles quadrilineatus (Hemiptera; Cicadellidae). To identify which of these effector proteins induce symptoms of phyllody and virescence, we individually expressed the effector genes in Arabidopsis. From this screen, we have identified a novel AY-WB effector protein, SAP54, that alters floral development, resulting in the production of leaf-like flowers that are similar to those produced by plants infected with this phytoplasma. This study offers novel insight into the effector profile of an insect-transmitted plant pathogen and reports to our knowledge the first example of a microbial pathogen effector protein that targets flower development in a host. 相似文献
994.
Small‐molecule auxin inhibitors that target YUCCA are powerful tools for studying auxin function 下载免费PDF全文
Yusuke Kakei Chiaki Yamazaki Masashi Suzuki Ayako Nakamura Akiko Sato Yosuke Ishida Rie Kikuchi Shouichi Higashi Yumiko Kokudo Takahiro Ishii Kazuo Soeno Yukihisa Shimada 《The Plant journal : for cell and molecular biology》2015,84(4):827-837
Auxin is essential for plant growth and development, this makes it difficult to study the biological function of auxin using auxin‐deficient mutants. Chemical genetics have the potential to overcome this difficulty by temporally reducing the auxin function using inhibitors. Recently, the indole‐3‐pyruvate (IPyA) pathway was suggested to be a major biosynthesis pathway in Arabidopsis thaliana L. for indole‐3‐acetic acid (IAA), the most common member of the auxin family. In this pathway, YUCCA, a flavin‐containing monooxygenase (YUC), catalyzes the last step of conversion from IPyA to IAA. In this study, we screened effective inhibitors, 4‐biphenylboronic acid (BBo) and 4‐phenoxyphenylboronic acid (PPBo), which target YUC. These compounds inhibited the activity of recombinant YUC in vitro, reduced endogenous IAA content, and inhibited primary root elongation and lateral root formation in wild‐type Arabidopsis seedlings. Co‐treatment with IAA reduced the inhibitory effects. Kinetic studies of BBo and PPBo showed that they are competitive inhibitors of the substrate IPyA. Inhibition constants (Ki) of BBo and PPBo were 67 and 56 nm , respectively. In addition, PPBo did not interfere with the auxin response of auxin‐marker genes when it was co‐treated with IAA, suggesting that PPBo is not an inhibitor of auxin sensing or signaling. We propose that these compounds are a class of auxin biosynthesis inhibitors that target YUC. These small molecules are powerful tools for the chemical genetic analysis of auxin function. 相似文献
995.
996.
Subcellular localization of glycolytic key enzymes in germinating castor bean endosperm 总被引:2,自引:0,他引:2
Phosphofructokinase and pyruvate kinase activities in castorbean endosperm increased during germination. Subcellular localizationof pyruvate kinase and phosphofructokinase in germinating endospermtissues was studied by differential and sucrose density gradientcentrifugation techniques. Eighty five percent or more of thepyruvate kinase and phosphofructokinase activities were locatedin cytosol. The remaining activities were mainly detected inproplastids. (Received June 30, 1977; ) 相似文献
997.
Tanno M Sakamoto J Miura T Shimamoto K Horio Y 《The Journal of biological chemistry》2007,282(9):6823-6832
998.
Shiratori H Ikeno H Ayame S Kataoka N Miya A Hosono K Beppu T Ueda K 《Applied and environmental microbiology》2006,72(5):3702-3709
A methanogenic bioreactor that utilized wastepaper was developed and operated at 55 degrees C. Microbial community structure analysis showed the presence of a group of clostridia that specifically occurred during the period of high fermentation efficiency. To isolate the effective cellulose digester, the sludge that exhibited high fermentation efficiency was inoculated into a synthetic medium that contained cellulose powder as the sole carbon source and was successively cultivated. A comprehensive 16S rRNA gene sequencing study revealed that the enriched culture contained various clostridia that had diverse phylogenetic positions. The microorganisms were further enriched by successive cultivation with filter paper as the substrate, as well as the bait carrier. A resultant isolate, strain EBR45 (= Clostridium sp. strain NBRC101661), was a new member of the order Clostridiales phylogenetically and physiologically related to Clostridium thermocellum and Clostridium straminisolvens. Specific PCR-based monitoring demonstrated that strain EBR45 specifically occurred during the high fermentation efficiency period in the original methanogenic sludge. Strain EBR45 effectively digested office paper in its pure cultivation system with a synthetic medium. 相似文献
999.
Miura T Fukami TA Hasegawa K Ono N Suda A Shindo H Yoon DO Kim SJ Na YJ Aoki Y Shimma N Tsukuda T Shiratori Y 《Bioorganic & medicinal chemistry letters》2011,21(19):5778-5783
Heat shock protein 90 (Hsp90) is a molecular chaperone which regulates maturation and stabilization of its substrate proteins, known as client proteins. Many client proteins of Hsp90 are involved in tumor progression and survival and therefore Hsp90 can be a good target for developing anticancer drugs. With the aim of efficiently identifying a new class of orally available inhibitors of the ATP binding site of this protein, we conducted fragment screening and virtual screening in parallel against Hsp90. This approach quickly identified 2-aminotriazine and 2-aminopyrimidine derivatives as specific ligands to Hsp90 with high ligand efficiency. In silico evaluation of the 3D X-ray Hsp90 complex structures of the identified hits allowed us to promptly design CH5015765, which showed high affinity for Hsp90 and antitumor activity in human cancer xenograft mouse models. 相似文献
1000.
Furuyama A Mochitate K 《American journal of physiology. Lung cellular and molecular physiology》2004,286(5):L939-L946
Hepatocyte growth factor (HGF) is a pulmotrophic factor for the regeneration of injured pulmonary tissue. We investigated the role of HGF in basement membrane formation during wound healing by immortalized alveolar type II epithelial cells that could form a continuous basement membrane when they were cultured on collagen fibrils in the presence of entactin-contaminated laminin-1. Cells cultured with 5.0 ng/ml HGF neither formed a continuous basement membrane on collagen fibrils nor maintained a continuous basement membrane architecture on a basement membrane substratum. The cells showed increased secretion of matrix metalloproteinase-9 and urokinase-type plasminogen activator, and the HGF-induced inhibition of basement membrane formation was attenuated by addition of 200 ng/ml tissue inhibitor of matrix metalloproteinase-1. Cells sequentially exposed to HGF and 1.0 ng/ml transforming growth factor-beta1 had enhanced basement membrane formation compared with those receiving these reagents in the reverse order or concurrently. HGF simultaneously stimulated proliferation and migration of the cells so that it advanced wound closure on the basement membrane substratum. The present results indicate that the role of HGF in wound healing is the stimulation of reepithelization, but this factor may also contribute to the degradation of the basement membrane. 相似文献