全文获取类型
收费全文 | 3093篇 |
免费 | 210篇 |
国内免费 | 2篇 |
专业分类
3305篇 |
出版年
2022年 | 14篇 |
2021年 | 35篇 |
2020年 | 26篇 |
2019年 | 33篇 |
2018年 | 37篇 |
2017年 | 32篇 |
2016年 | 65篇 |
2015年 | 123篇 |
2014年 | 114篇 |
2013年 | 173篇 |
2012年 | 228篇 |
2011年 | 196篇 |
2010年 | 132篇 |
2009年 | 142篇 |
2008年 | 220篇 |
2007年 | 246篇 |
2006年 | 223篇 |
2005年 | 239篇 |
2004年 | 219篇 |
2003年 | 186篇 |
2002年 | 164篇 |
2001年 | 26篇 |
2000年 | 14篇 |
1999年 | 26篇 |
1998年 | 29篇 |
1997年 | 32篇 |
1996年 | 22篇 |
1995年 | 22篇 |
1994年 | 19篇 |
1993年 | 15篇 |
1992年 | 27篇 |
1991年 | 18篇 |
1990年 | 19篇 |
1989年 | 17篇 |
1988年 | 17篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 13篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 11篇 |
1980年 | 13篇 |
1979年 | 9篇 |
1978年 | 12篇 |
1977年 | 8篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1973年 | 4篇 |
排序方式: 共有3305条查询结果,搜索用时 15 毫秒
91.
The voltage-sensor domain (VSD) is a functional module that undergoes structural transitions in response to membrane potential changes and regulates its effectors, thereby playing a crucial role in amplifying and decoding membrane electrical signals. Ion-conductive pore and phosphoinositide phosphatase are the downstream effectors of voltage-gated channels and the voltage-sensing phosphatase, respectively. It is known that upon transition, the VSD generally acts on the region C-terminal to S4. However, whether the VSD also induces any structural changes in the N-terminal region of S1 has not been addressed directly. Here, we report the existence of such an N-terminal effect. We used two distinct optical reporters—one based on the Förster resonance energy transfer between a pair of fluorescent proteins, and the other based on fluorophore-labeled HaloTag—and studied the behavior of these reporters placed at the N-terminal end of the monomeric VSD derived from voltage-sensing phosphatase. We found that both of these reporters were affected by the VSD transition, generating voltage-dependent fluorescence readouts. We also observed that whereas the voltage dependencies of the N- and C-terminal effects appear to be tightly coupled, the local structural rearrangements reflect the way in which the VSD is loaded, demonstrating the flexible nature of the VSD. 相似文献
92.
93.
Teruyoshi Matoba Hozumi Yoshida Daizo Yonezawa 《Bioscience, biotechnology, and biochemistry》2013,77(4):979-986
Casein and egg albumin were allowed to react with methyl linoleate (ML) at a relative humidity (RH) of 0% or 80% at 50°C for 10 days (protein: ML= 1:0.2 or 1:1, w/w). Changes in the molecular sizes of the reacting proteins were examined by gel filtration and gel electrophoresis. Both proteins showed similar changes, whereas the reaction at RH 80% (protein: ML= 1:1) resulted in insolubilization because of polymerization. Changes in the amino acid residues of the reacting proteins were investigated after acid (6 n HC1) and enzymatic (pepsin-pancreatin, followed by aminopeptidase-prolidase) hydrolyses. Insignificant changes were observed in the amino acid composition of proteins reacted at RH 0%. After reaction at RH 80% (protein: ML =1:1), Lys, His and Met were the only amino acids affected. The percentage loss of these amino acids after acid hydrolysis was Lys (22%), His (41%), Met (9%) for casein and Lys (22%), His (31%), Met (1%) for egg albumin. This percentage loss after enzymatic hydrolysis was Lys (41%), His (49%), Met (94%) for casein and Lys (37%), His (42%), Met (88%) for egg albumin. Some differences between our results and other researchers were also discussed. 相似文献
94.
Takaaki Finn Akiko Nakazawa Naoki Sumi Hiroaki Tani Akikazu Ando Minoru Yabuki 《Bioscience, biotechnology, and biochemistry》2013,77(12):2747-2753
A purple non-sulfur bacterium, Rhodopseudomonas sp. No. 7, was isolated from n-propanol–enrichment cultures under anaerobic-light conditions. Strain No. 7 can produce hydrogen from alcohols. The rate of hydrogen production from n-propanol was 34 μl/hr/mg dry cells. Strain No. 7 showed multiplication by budding and the best growth on n-propanol among other organic compounds tested. But its growth on n-propanol was poor under aerobic-dark conditions. NAD-linked alcohol dehydrogenase, NAD-linked aldehyde dehydrogenase, acyl-CoA synthetase and malate synthetase were found in strain No. 7. These enzymes were constitutive. On the other hand, isocitrate lyase was induced in cells grown on ethanol but not on n-propanol. No activity of phenazine methosulfate-linked alcohol dehydrogenase was detected in strain No. 7. 相似文献
95.
96.
Seizo Sumida Yoshio Hisada Akiko Kometani Junshi Miyamoto 《Bioscience, biotechnology, and biochemistry》2013,77(9):2127-2136
Using 3-(3′,5′-dichlorophenyl)-5,5-dimethyloxazolidine-2,4-dione labeled with 14C or 3H, absorption, excretion, and tissue distribution in male Wistar rats were studied, and metabolites excreted were identified. At the dosage rates of 100, 300, 1000 and 3000 mg/kg, the maximum excretion of orally administered radioactivity occurred within 24 hr. Increase in the dosage rate was paralleled by decrease in the proportion of urinary elimination. Essentially all the radioactivity was excreted in 2 weeks. DDOD level was generally low in most tissues. Adipose tissue contained higher radioactivity compared with others. Most of the urinary metabolites identified were characterized by hydroxylation at the 4′ position of the benzene ring moiety, and hydrolytic or oxidative modification of the oxazolidine ring portion. 相似文献
97.
98.
99.
100.
Hironao Yamada Sakiko Mori Takeshi Miyakawa Ryota Morikawa Fumihiko Katagiri Kentaro Hozumi Yamato Kikkawa Motoyoshi Nomizu Masako Takasu 《PloS one》2016,11(2)
Peptides with cell attachment activity are beneficial component of biomaterials for tissue engineering. Conformational structure is one of the important factors for the biological activities. The EF1 peptide (DYATLQLQEGRLHFMFDLG) derived from laminin promotes cell spreading and cell attachment activity mediated by α2β1 integrin. Although the sequence of the EF2 peptide (DFATVQLRNGFPYFSYDLG) is homologous sequence to that of EF1, EF2 does not promote cell attachment activity. To determine whether there are structural differences between EF1 and EF2, we performed replica exchange molecular dynamics (REMD) simulations and conventional molecular dynamics (MD) simulations. We found that EF1 and EF2 had β-sheet structure as a secondary structure around the global minimum. However, EF2 had variety of structures around the global minimum compared with EF1 and has easily escaped from the bottom of free energy. The structural fluctuation of the EF1 is smaller than that of the EF2. The structural variation of EF2 is related to these differences in the structural fluctuation and the number of the hydrogen bonds (H-bonds). From the analysis of H-bonds in the β-sheet, the number of H-bonds in EF1 is larger than that in EF2 in the time scale of the conventional MD simulation, suggesting that the formation of H-bonds is related to the differences in the structural fluctuation between EF1 and EF2. From the analysis of other non-covalent interactions in the amino acid sequences of EF1 and EF2, EF1 has three pairs of residues with hydrophobic interaction, and EF2 has two pairs. These results indicate that several non-covalent interactions are important for structural stabilization. Consequently, the structure of EF1 is stabilized by H-bonds and pairs of hydrophobic amino acids in the terminals. Hence, we propose that non-covalent interactions around N-terminal and C-terminal of the peptides are crucial for maintaining the β-sheet structure of the peptides. 相似文献