全文获取类型
收费全文 | 3362篇 |
免费 | 220篇 |
国内免费 | 2篇 |
出版年
2022年 | 12篇 |
2021年 | 40篇 |
2020年 | 25篇 |
2019年 | 33篇 |
2018年 | 40篇 |
2017年 | 33篇 |
2016年 | 64篇 |
2015年 | 126篇 |
2014年 | 118篇 |
2013年 | 186篇 |
2012年 | 231篇 |
2011年 | 217篇 |
2010年 | 135篇 |
2009年 | 151篇 |
2008年 | 229篇 |
2007年 | 248篇 |
2006年 | 233篇 |
2005年 | 254篇 |
2004年 | 228篇 |
2003年 | 199篇 |
2002年 | 181篇 |
2001年 | 42篇 |
2000年 | 36篇 |
1999年 | 46篇 |
1998年 | 34篇 |
1997年 | 44篇 |
1996年 | 29篇 |
1995年 | 33篇 |
1994年 | 29篇 |
1993年 | 24篇 |
1992年 | 41篇 |
1991年 | 19篇 |
1990年 | 26篇 |
1989年 | 19篇 |
1988年 | 24篇 |
1987年 | 11篇 |
1986年 | 16篇 |
1985年 | 16篇 |
1984年 | 15篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 16篇 |
1980年 | 11篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 8篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 6篇 |
排序方式: 共有3584条查询结果,搜索用时 31 毫秒
101.
102.
103.
Kozi Asada 《Bioscience, biotechnology, and biochemistry》2013,77(8):1659-1660
Acidic heteropolysaccharides, d-glucurono-d-xylo-d-mannans were isolated from the water- and alkaline extracts of the fruit body of Tremella fuciformis Berk. Similar polysaccharides were isolated from the growing culture of the haploid cells of two strains (T–19 and T–7) of T. fuciformis, when they were cultured in sucrose or glucose-yeast extract medium. The extracellular polysaccharides contain, d-glucuronic acid, d-xylose and d-mannose [molar ratios, 1.3: 1.0: 3.5 (T–7) and 0.8: 1.0: 2.1 (T–19)], and, in addition, small proportions of l-fucose and O-acetyl groups. Methylation and Smith degradation studies indicated that both fruit body and extracellular polysaccharides are built up of α-(1 → 3)-linked d-mannan backbone chain to which β-linked d-glucuronic acid and single or short chains of β-(1 → 2)-linked d-xylose residues are attached at the C–2 position. l-fucose residues in the extracellular polysaccharides may form the single branches. The structural features of these polysaccharides are discussed in comparison with the similar polysaccharides from other fungi. 相似文献
104.
Masanori Asada Kazuhiro Morimoto Kazuhiro Nakanishi Ryuichi Matsuno Atsuo Tanaka Akira Kimura 《Bioscience, biotechnology, and biochemistry》2013,77(8):1773-1774
Arthrobacter simplex was screened as an α-keto-δ-guanidinovalerate (ketoarginine) assimilating organism. A characteristic feature was its growth on ketoarginine as a carbon source; it began to grow after an extremely long lag. Its growth was stimulated by addition of 0.02% yeast extract to the medium.The results indicated the transamination of arginine-α-ketoglutarate (α-KGA) and the hydrolyzing reaction of ketoarginine into α-keto-δ-aminovalerate and urea. Two intermediates, ketoarginine and α-keto-δ-aminovalerate, were isolated and identified by various procedures. Coupling of the two reactions was demonstrated in cell-free extracts of arginine-grown cells; ketoarginine formed from arginine by transamination with α-KGA was hydrolyzed directly to α-keto-δ-aminovalerate and urea. The metabolic routes of arginine in microorganisms were discussed. 相似文献
105.
Takaaki Finn Akiko Nakazawa Naoki Sumi Hiroaki Tani Akikazu Ando Minoru Yabuki 《Bioscience, biotechnology, and biochemistry》2013,77(12):2747-2753
A purple non-sulfur bacterium, Rhodopseudomonas sp. No. 7, was isolated from n-propanol–enrichment cultures under anaerobic-light conditions. Strain No. 7 can produce hydrogen from alcohols. The rate of hydrogen production from n-propanol was 34 μl/hr/mg dry cells. Strain No. 7 showed multiplication by budding and the best growth on n-propanol among other organic compounds tested. But its growth on n-propanol was poor under aerobic-dark conditions. NAD-linked alcohol dehydrogenase, NAD-linked aldehyde dehydrogenase, acyl-CoA synthetase and malate synthetase were found in strain No. 7. These enzymes were constitutive. On the other hand, isocitrate lyase was induced in cells grown on ethanol but not on n-propanol. No activity of phenazine methosulfate-linked alcohol dehydrogenase was detected in strain No. 7. 相似文献
106.
Kazuaki Manabe Mitsuyoshi Morii Masaru Honjo Masaharu Ohoka Kouichiro Fushimi Akiko Sawakura 《Bioscience, biotechnology, and biochemistry》2013,77(8):2261-2267
Bacillus subtilis 1A20 transformed with a hybrid plasmid, pNP150, to which a DNA fragment from Bacillus amyloliquefaciens F was attached, produced a large amount of a neutral protease. To identify the origin of the gene specifying this neutral protease, neutral proteases from B. amyloliquefaciens F, B. subtilis NP58 (a derivative of Marburg 6160), and B. subtilis 1A20 transformed with pNP150 were purified. We investigated their immunological properties and primary structures.The proteases from these two species were indistinguishable by chromatography, but they were distinguishable from each other by SDS-polyacrylamide gel electrophoresis and double immunodiffusion. Amino acid sequencing of these two proteases by Edman degradation showed that there were four substitutions in the 20-residue amino acid sequence from the N-termini.Neutral protease from the transformant had the same immunological characteristics and N-terminal amino acid sequence as that from B. amyloliquefaciens. These results meant that the gene in question was derived from a gene specifying the neutral protease in this bacterium. 相似文献
107.
Kozi Asada Masa-aki Takahashi Mieko Nagate 《Bioscience, biotechnology, and biochemistry》2013,77(2):471-473
Allosamidin, a product of Streptomyces sp. No 1713, inhibited Bombyx mori chitinase specifically in a competitive way with a Ki o f about 0.1 μm. The effect of allosamidin on chitinases from r Streptomyces griseus and Serratia marcescens was weaker, about 1/500 that on B. mori chitinase. Allosamidin did not inhibit yam chitinase, lysozymes of hen egg-white or human urine, or B. mori α-N-acetyl-d-glucosaminidase. The results suggest that allosamidin is a specific inhibitor of the insect chitinase. 相似文献
108.
109.
Yasuhiko Asada Katsuyuki Tanizawa Yoshiyasu Kawabata Haruo Misono Kenji Soda 《Bioscience, biotechnology, and biochemistry》2013,77(6):1513-1514
Soybean 7S and 11S globulins were stored at relative humidities (RHs) of 11% and 96% at 50°C. The redispersibility of the proteins at RH 96% decreased in a short time. However, it did not decrease, when stored for 45 days at RH 11%. Gel filtration showed that the proteins polymerized during storage. The effects of urea, sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (2-ME) on the redispersibilities of the proteins at RH 96% showed that the hydrogen, hydrophobic and disulfide bonds participate in the polymerization of 7S globulin, and that the disulfide bond is strongly related to the polymerization of 11S globulin. Redispersibility was restored with 2-ME in both the 7S and 11S globulins and some of the proteins in the supernatant redispersed with 2-ME were observed to be similar to the native ones with respect to the gel filtration, electrophoretic behavior and circular dichroism spectrum. 相似文献
110.
Seizo Sumida Yoshio Hisada Akiko Kometani Junshi Miyamoto 《Bioscience, biotechnology, and biochemistry》2013,77(9):2127-2136
Using 3-(3′,5′-dichlorophenyl)-5,5-dimethyloxazolidine-2,4-dione labeled with 14C or 3H, absorption, excretion, and tissue distribution in male Wistar rats were studied, and metabolites excreted were identified. At the dosage rates of 100, 300, 1000 and 3000 mg/kg, the maximum excretion of orally administered radioactivity occurred within 24 hr. Increase in the dosage rate was paralleled by decrease in the proportion of urinary elimination. Essentially all the radioactivity was excreted in 2 weeks. DDOD level was generally low in most tissues. Adipose tissue contained higher radioactivity compared with others. Most of the urinary metabolites identified were characterized by hydroxylation at the 4′ position of the benzene ring moiety, and hydrolytic or oxidative modification of the oxazolidine ring portion. 相似文献