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排序方式: 共有198条查询结果,搜索用时 125 毫秒
101.
Nishiguchi Akihiro Murakami Akikazu Azuma Takachika Oda Masayuki 《The protein journal》2022,41(2):293-303
The Protein Journal - Somatic hypermutation (SHM) is one of the driving forces that increases antibody (Ab) affinity. We studied the effects of SHM on thermostability and affinity using three... 相似文献
102.
103.
Akikazu Hatanaka Osao Adachi Toshikazu Chiyonobu Minoru Ameyama 《Bioscience, biotechnology, and biochemistry》2013,77(7):1142-1143
The isomeric ratio and level of natural xanthoxin (XAN) in tomato plants (Lycopersicon esculentum) were examined by a more reliable analytical method than has been reported before. Efforts were made to avoid artificial isomerization between c-XAN and t-XAN throughout the isolation, derivatization and GC-MS procedures. Natural XAN was separated from contaminating chlorophylls before rev. HPLC purification, derivatized to abscisic acid methyl ester (MeABA) in four chemical steps, and quantified with the deuterium-labeled internal standards on clear and reproducible full GC-EI-MS. It was revealed that the isomeric composition of natural XAN was exclusively shifted to c-XAN. The level of c-XAN was higher and more significantly induced by water stress in older plants. The significant role of c-XAN as an ABA biosynthetic precursor is suggested. 相似文献
104.
The activity of the enzyme system (E2-I) producing C6-aldehydes from C18-unsaturated fatty acids was investigated using about 40 plants. Green leaves of dicotyledonous plants belonging to the Sphenopsida, Pteropsida Theaceae and Leguminosae showed a high enzyme (E2-I) activity but edible leafy vegetables and fuits and monocotyledonous plants showed a low activity. Seasonal changes in the enzyme (E2-I) activities were observed. The concentrations of cis-3-hexenol (leaf alcohol) and trans-2-hexenal (leaf aldehyde) and the enzyme (E2-I) activities showed a correlation; high concentrations were observed in the summer but they were low in the winter. 相似文献
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106.
Akikazu Hatanaka Tadahiko Kajiwara Minoru Ohno 《Bioscience, biotechnology, and biochemistry》2013,77(8):964-972
2-Methyl-4, 6-cyclohexadienaldehyde and n-butyraldehyde were treated with sodium in p-xylene to yield the aromatized “leaf alcohol reaction” product, 2-methyl-benzylalcohol, in a better yield than that with the cyclohexadienaldehyde alone. n-Butyric acid isolated from the reaction mixture unequivocally showed the operation of the “crossed Cannizzaro disproportionation” in this reaction, aliphatic aldehyde serving as the hydride donor. 2-Propyl-5-ethyl-4, 6-cyclohexadienaldehyde was obtained by the NaOH/H2O-EtOH Michael-Aldol condensation of leaf aldehyde, gave 2-propyl-5-ethyl-benzylalcohol along with caproic acid.On the basis of “leaf alcohol-reaction” mechanism, it was obtained following benzyl-alkohols; 2-methyl-, 2-propyl-, 2-methyl-5-ethyl-, 2-propyl-5-ethyl-benzylalcohol, from leaf alcohol and crotylalcohol. 相似文献
107.
Sakudo A Suganuma Y Kobayashi T Onodera T Ikuta K 《Biochemical and biophysical research communications》2006,341(2):279-284
Although several methods, including enzyme-linked immunosorbent assay, polymerase chain reaction, immunofluorescent assay, and Western blotting, have been used for the diagnosis of viral infections, none of them is ideal in terms of cost-effectiveness, speed, and accuracy. Currently, the rate of outbreak of emerging viruses is increasing and therefore the development and establishment of analytical methods for such viral infections are becoming more important. Near-infrared (NIR) spectroscopy is a fast, multicomponent assay that enables non-invasive, non-destructive analysis. Recently, the diagnosis of viral infections using NIR spectroscopy has been attempted. In this review, the potential of the NIR method in the medical and virological fields is discussed. 相似文献
108.
Sakudo A Tsenkova R Tei K Onozuka T Ikuta K Yoshimura E Onodera T 《Bioscience, biotechnology, and biochemistry》2006,70(7):1578-1583
The near-infrared (NIR) spectra of such metals as Cu(II), Mn(II), Zn(II) and Fe(III) in HNO(3) in the 700-1,860 nm region were subjected to a partial least-squares regression analysis and leave-out cross-validation to develop chemometric models. The models yielded a coefficient of determination in cross validation of 0.9744 [Cu(II)], 0.9631 [Mn(II)], 0.9154 [Zn(II)] and 0.741 [Fe(III)]. The regression coefficients for Cu(II), Mn(II) and Zn(II), but not for Fe(III), showed strong negative peaks at around 1,050-1,200 nm, a zone where spectral bands have been reported to decrease with increasing pH value. A positive peak at around 710-750 nm, which may have been due to water absorption, was observed in regression coefficients of Cu(II), Mn(II) and Zn(II) but not in Fe(III), while a negative peak was observed in that for Fe(III) at around 710-750 nm. These results indicate that the divalent cations [Cu(II), Mn(II) and Zn(II)] showed different absorption in the NIR region from the trivalent cation [Fe(III)], suggesting that the vibration mode of water, which mirrors the interaction between cations and water, may be influenced by valency. 相似文献
109.
Cytochrome c oxidases of prion protein (PrP) gene-deficient (Prnp(-/-)) and Prnp(+/+) mice were examined in vivo and in vitro. Non-invasive near-infrared spectra revealed that oxidation of copper and heme a+a(3) in cytochrome c oxidase of Prnp(-/-) mice was similar to that in Prnp(+/+) mice. Biochemical analysis of mitochondrial fractions also supported the results. PrP might not be involved in regulation of cytochrome c oxidase. 相似文献
110.
Es wurde aufgeklärt, daβ die im Tee-blättern weit verbreitet vorkommende natürliche Blätter-alcohol-fraction aus einern Gemisch der cis, trans-Isomere besteht, wobei bisher das cis-Isomere besteht, wobei bisher das cis-Isomere stark überwiegt. 相似文献