全文获取类型
收费全文 | 301篇 |
免费 | 16篇 |
出版年
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 1篇 |
2020年 | 6篇 |
2019年 | 2篇 |
2018年 | 6篇 |
2017年 | 9篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 18篇 |
2012年 | 16篇 |
2011年 | 18篇 |
2010年 | 15篇 |
2009年 | 9篇 |
2008年 | 19篇 |
2007年 | 11篇 |
2006年 | 14篇 |
2005年 | 10篇 |
2004年 | 9篇 |
2003年 | 15篇 |
2002年 | 17篇 |
2001年 | 9篇 |
2000年 | 9篇 |
1999年 | 10篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 6篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1981年 | 6篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1977年 | 1篇 |
1975年 | 3篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有317条查询结果,搜索用时 15 毫秒
71.
Lipoperoxides, vitamin E, and activities of superoxide dismutase, glutathione peroxidase, and catalase in regenerating rat liver 总被引:2,自引:0,他引:2
Lipoperoxides in homogenates of regenerating rat liver increased from 6 hours after the operation and reached a peak (about 7 times the control level) 18-24 hours after the operation. The concentration of blood lipoperoxides rapidly decreased after the operation. The enzymatic activities of superoxide dismutase, catalase, and glutathione peroxidase, and vitamin E content in regenerating livers were also determined. Among these antioxidant factors, the catalase level changed markedly. 相似文献
72.
T Etoh K Takehara A Igarashi Y Ishibashi 《Biochemical and biophysical research communications》1989,162(3):1010-1016
The effects of various growth factors on endothelial cell survival in vitro were studied. Using rat heart endothelial cells, the cell survival curves were obtained; the cells were cultured until confluent, the medium was changed to serum-free medium with or without growth factors, and the cells were counted after 3, 6, 9, and 12 days. Transforming growth factor-beta, which is known as a potent growth inhibitor for vascular endothelial cells, shortened the rat heart endothelial cell's survival period, while epidermal growth factor or transforming growth factor-alpha prolonged survival. Insulin did not affect the rat heart endothelial cell's survival. Our data indicate that growth factors play a role not only in cell proliferation but also in cell survival in vitro. In addition, elevated levels of growth inhibitors such as transforming growth factor-beta may cause tissue damage in vivo by affecting cell survival. 相似文献
73.
Seiichi Tawara Tomohide Tatsumi Sadaharu Iio Ichizou Kobayashi Minoru Shigekawa Hayato Hikita Ryotaro Sakamori Naoki Hiramatsu Eiji Miyoshi Tetsuo Takehara 《PloS one》2016,11(3)
Fucosylated haptoglobin (Fuc-Hpt) and Mac-2 binding protein (Mac-2 bp) are identified as cancer biomarkers, based on the results from a glyco-proteomic analysis. Recently, we reported that these glyco-biomarkers were associated with liver fibrosis and/or ballooning hepatocytes in patients with nonalcoholic fatty liver disease (NAFLD). We evaluated the ability of these glycoproteins to estimate liver fibrosis in 317 patients with chronic hepatitis C. We measured the serum Fuc-Hpt and Mac-2 bp levels using a lectin-antibody ELISA and ELISA, respectively. The serum levels of both Fuc-Hpt and Mac-2 bp increased with the progression of liver fibrosis. The multivariate analysis revealed that Mac-2 bp was an independent factor associated with moderate liver fibrosis (F ≥ 2). In contrast, Fuc-Hpt was an independent factor associated with advanced liver fibrosis (F ≥ 3). In terms of evaluating liver fibrosis, the serum levels of these glycomarkers were correlated with well-known liver fibrosis indexes, such as the aspartate aminotransferase to platelet ratio index (APRI) and Fibrosis-4 (FIB4) index. An assay that combined the APRI or FIB4 index and the Fuc-Hpt or Mac-2 bp levels increased the AUC value for diagnosing hepatic fibrosis. Interestingly, the cumulative incidence of hepatocellular carcinoma (HCC) was significantly higher in the patients with elevated serum levels of Fuc-Hpt and Mac-2 bp. In conclusion, both Fuc-Hpt and Mac-2 bp could be useful glyco-biomarkers of liver fibrosis and predictors of HCC in patients with chronic hepatitis C. 相似文献
74.
75.
TGF-beta inhibition of endothelial cell proliferation: alteration of EGF binding and EGF-induced growth-regulatory (competence) gene expression 总被引:31,自引:0,他引:31
Transforming growth factor-beta (TGF-beta) inhibits the growth of endothelial cells derived from various sources, including human umbilical vein, bovine aorta, and rat heart. Long-term exposure of rat heart endothelial cells to TGF-beta also induces dramatic changes in morphology that are characteristic of senescent cells. These changes are accompanied by a decrease in the number of high-affinity receptors for epidermal growth factor (EGF), with almost no change in total receptor number. Additionally, the EGF-induced expression of specific competence genes (c-myc, JE, KC) is decreased, whereas the induction of c-fos gene expression by EGF is unaltered by TGF-beta treatment. These data suggest that growth inhibitors such as TGF-beta may act by altering the cell's response to growth-stimulatory factors. 相似文献
76.
Yoko Ino Eiji Kinoshita Emiko Kinoshita-Kikuta Tomoko Akiyama Yusuke Nakai Kohei Nishino Makoto Osada Akihide Ryo Hisashi Hirano Tohru Koike Yayoi Kimura 《Proteomics》2022,22(7):2100216
Information about phosphorylation status can be used to prioritize and characterize biological processes in the cell. Various analytical strategies have been proposed to address the complexity of phosphorylation status and comprehensively identify phosphopeptides. In this study, we evaluated four strategies for phosphopeptide enrichment, using titanium dioxide (TiO2) and Phos-tag ligand particles from in-gel or in-solution digests prior to mass spectrometry-based analysis. Using TiO2 and Phos-tag magnetic beads, it was possible to enrich phosphopeptides from in-gel digests of phosphorylated ovalbumin separated by Phos-tag SDS-PAGE or in-solution serum digests, while minimizing non-specific adsorption. The tip-column strategy with TiO2 particles enabled enrichment of phosphopeptides from in-solution digests of whole-cell lysates with high efficiency and selectivity. However, the tip-column strategy with Phos-tag agarose beads yielded the greatest number of identified phosphopeptides. The strategies using both types of tip columns had a high degree of overlap, although there were differences in selectivity between the identified phosphopeptides. Together, our results indicate that multi-enrichment strategies using TiO2 particles and Phos-tag agarose beads are useful for comprehensive phosphoproteomic analysis. 相似文献
77.
Shin-Ichi Seki Hajime Takano Kazuto Kawakami Nobuhiko Kotaka Akira Endo Kenji Takehara 《Conservation Genetics》2007,8(5):1109-1121
The Japanese wood pigeon (Columba janthina) is endemic to the islands of East Asia and it is included in the Japanese and Asian Red Lists because of its narrow habitat
range that is restricted to mature forests on small islands and because of the destruction of these habitats. We examined
the genetic structure of Columba janthina by studying 463 base pairs of the mitochondrial control region sequences. We analyzed 154 samples from eight populations
and identified 27 haplotypes. Three population groups were identified based on the pairwise ΦST values. A substantial gene flow, as reflected by the low and non-significant ΦST values, is maintained among the northern group that includes six populations found on the Okinawa, Tokara, Goto, Setouchi,
Oki, and Izu islands. In contrast, the southeastern group found on the Ogasawara Islands had large ΦST values with every population from other regions (0.910 < ΦST < 0.962). The southwestern group found on the Sakishima Islands also had significant but small ΦST values with every population from the northern group (0.081 < ΦST < 0.205). The Mantel test showed a highly significant correlation between the ΦST values and the route length of the habitat network, as well as the linear distance with correction of the habitat gap effect,
indicating the importance of the closely connected structure of the habitats. The three groups mentioned above could be considered
as independent management units, and the southeastern group has the highest conservation priority due to its narrow distribution
range and small population size.
Electronic Supplementary Material The online version of this article (doi: ) contains supplementary material, which is available to authorized users. 相似文献
78.
Matsuda K Makise M Sueyasu Y Takehara M Asano T Mizushima T 《FEMS yeast research》2007,7(8):1263-1269
Origin recognition complex (ORC), a six-protein complex (Orc1p-6p), is the most likely initiator of chromosomal DNA replication in eukaryotes. Although ORC of Saccharomyces cerevisiae has been studied extensively from biochemical and genetic perspectives, its quaternary structure remains unknown. Previous studies suggested that ORC has functions other than DNA replication, such as gene silencing, but the molecular mechanisms of these functions have not been determined. In this study, we used yeast two-hybrid analysis to examine the interaction between ORC subunits and to search for ORC-binding proteins. As well as the known Orc4p-Orc5p interaction, we revealed strong interactions between Orc2p and Ord3p (2p-3p), Orc2p and Ord5p (2p-5p), Orc2p and Ord6p (2p-6p) and Orc3p and Ord6p (3p-6p) and weaker interactions between Orc1p and Ord4p (1p-4p), Orc3p and Ord4p (3p-4p), Orc2p and Ord3p (3p-5p) and Orc5p and Ord3p (5p-6p). These results suggest that 2p-3p-6p may form a core complex. Orc2p and Orc6p are phosphorylated in vivo, regulating initiation of DNA replication. However, replacing the phosphorylated amino acid residues with others that cannot be phosphorylated, or that mimic phosphorylation, did not affect subunit interactions. We also identified several proteins that interact with ORC subunits; Sir4p and Mad1p interact with Orc2p; Cac1p and Ykr077wp with Orc3p; Rrm3p and Swi6p with Orc5p; and Mih1p with Orc6p. We discuss roles of these interactions in functions of ORC. 相似文献
79.
Hoshino T Namba T Takehara M Murao N Matsushima T Sugimoto Y Narumiya S Suzuki T Mizushima T 《Journal of neurochemistry》2012,120(5):795-805
Amyloid-β peptide (Aβ), which is generated by the β- and γ-secretase-mediated proteolysis of β-amyloid precursor protein (APP), plays an important role in the pathogenesis of Alzheimer's disease (AD). We recently reported that prostaglandin E(2) (PGE(2) ) stimulates the production of Aβ through both EP(2) and EP(4) receptors and that activation of the EP(4) receptor stimulates Aβ production through endocytosis and activation of γ-secretase. We here found that transgenic mice expressing mutant APP (APP23) mice showed a greater or lesser apparent cognitive deficit when they were crossed with mice lacking EP(2) or EP(4) receptors, respectively. Mice lacking the EP(4) receptor also displayed lower levels of Aβ plaque deposition and less neuronal and synaptic loss than control mice. Oral administration of a specific EP(4) receptor antagonist, AE3-208 to APP23 mice, improved their cognitive performance, as well as decreasing brain levels of Aβ and suppressing endocytosis and activation of γ-secretase. Taken together, these results suggest that inhibition of the EP(4) receptor improves the cognitive function of APP23 mice by suppressing Aβ production and reducing neuronal and synaptic loss. We therefore propose that EP(4) receptor antagonists, such as AE3-208, could be therapeutically beneficial for the prevention and treatment of AD. 相似文献
80.