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81.
Takashi Sugita Koichi Makimura Akemi Nishikawa Katsuhisa Uchida Hideyo Yamaguchi Takako Shinoda 《Microbiology and immunology》1997,41(7):571-573
We determined the partial sequences of large subunit rDNA of a new yeast species, Trichosporon domesticum, which was isolated from the house of a summer-type hypersensitivity pneumonitis patient. Phylogenetically, T. domesticum was positioned in the taxonomic group containing T. montevideense and T. brassicae, which indicated an identical serotype. A phylogenetic relationship among all species of the genus Trichosporon which belong to the basidiomycetous yeast is clarified. 相似文献
82.
83.
Mukohata Yasuo; Yagi Takao; Higashida Mitsuhiro; Matsuno Akemi; Sugiyama Yasuo 《Plant & cell physiology》1974,15(4):619-628
Spinach chloroplasts were isolated and stored in a medium containing0.5 M choline chloride. The properties of these chloroplastswere compared with those of chloroplasts prepared in an ordinarysucrose medium. In marked contrast to sucrose-prepared chloroplasts, choline-preparedchloroplasts did not show "conventional uncoupling" (stimulationof ferricyanide reduction paralleling the inactivation of phosphorylation)after transient warming in the temperature range between 20?and 55?C. The thermal stability of the oxygen evolving system, the vulnerabilityof the lipophilic structure in the thylakoids to alcohol duringthe warming treatment, and the effects of amine on photophosphorylationwere similar in both chloroplast preparations. After the warming treatment, the degree of swelling in chloroplastswas larger and the intermediate electron transport systme (fromreduced 2,6-dichlorophenolindophenol to methyl viologen) wasmore stable against uncoupling, in choline-prepared chloroplaststhan in sucrose-prepared ones. The presence and absence of "conventional uncoupling" in twochloroplast preparations were ascribed to differences in thermalstability. In choline-prepared chloroplasts the uncoupling temperatureof the electron transport system was higher than the inactivationtemperature of the oxygen evolving system, but it was lowerin the sucrose-prepared ones. (Received February 13, 1974; ) 相似文献
84.
Yasuo Mukohata Takao Yagi Mitsuhiro Higashida Akemi Matsuno 《Journal of bioenergetics and biomembranes》1973,4(5):479-490
Spinach chloroplasts pre-incubated at various temperatures, changed their photosynthetic activities (measured at 15°C) as the incubation temperature was raised; these activities showed characteristic activitytemperature profiles as follows:
- The profiles were shifted to lower temperatures if various aliphatic compounds were present in the incubation mixture.
- In general, the extent of the shift was proportional to the product of the concentration and the partition coefficient of the compound, i.e. to the concentration of the compound partitioned in the lipophilic chloroplast phase.
85.
Bai Songling Tuan Pham Anh Tatsuki Miho Yaegaki Hideaki Ohmiya Akemi Yamamizo Chihiro Moriguchi Takaya 《Plant Molecular Biology Reporter》2016,34(1):257-264
Plant Molecular Biology Reporter - Transgenic approach is an excellent way for the clarification of gene function, but it is generally difficult to create transgenic plants for most of the fruit... 相似文献
86.
Sana Ben Othman Nakako Katsuno Akemi Kitayama Makoto Fujimura Kohji Kitaguchi 《Free radical research》2016,50(9):949-958
Oxidative stress is involved in the development of aging-related diseases, such as neurodegenerative diseases. Dietary antioxidants that can protect neuronal cells from oxidative damage play an important role in preventing such diseases. Previously, we reported that water-soluble fractions purified from defatted sesame seed flour exhibit good antioxidant activity in vitro. In the present study, we investigated the protective effects of white and gold sesame seed water-soluble fractions (WS-wsf and GS-wsf, respectively) against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydrogen peroxide (H2O2) induced oxidative stress in human neuroblast SH-SY5Y cells. Pretreatment with WS-wsf and GS-wsf did not protect cells against AAPH-induced cytotoxicity, while simultaneous co-treatment with AAPH significantly improved cell viability and inhibited membrane lipid peroxidation. These results suggest that WS-wsf and GS-wsf protect cells from AAPH-induced extracellular oxidative damage via direct scavenging of peroxyl radicals. When oxidative stress was induced by H2O2, pretreatment WS-wsf and GS-wsf significantly enhanced cell viability. These results suggest that in addition to radical scavenging, WS-wsf and GS-wsf enhance cellular resistance to intracellular oxidative stress by activation of the Nrf-2/ARE pathway as confirmed by the increased Nrf2 protein level in the nucleus and increased heme oxygenase 1 (HO-1) mRNA expression. The roles of ferulic and vanillic acids as bioactive antioxidants in these fractions were also confirmed. In conclusion, our results indicated that WS-wsf and GS-wsf, which showed antioxidant activity in vitro, are also efficient antioxidants in a cell system protecting SH-SY5Y cells against both extracellular and intracellular oxidative stress. 相似文献
87.
Reiko Ando Noriyo Tokuda Tokunori Yamamoto Kazutaka Ikeda Noboru Hashimoto Ryo Taguchi Xiaoen Fan Keiko Furukawa Yukio Niimura Akemi Suzuki Momokazu Goto Koichi Furukawa 《Glycoconjugate journal》2016,33(2):169-180
In this study, we immunized Gb3/CD77 synthase gene (A4galt) knockout (KO) mice with glycosphingolipids (GSLs) extracted from 3 renal cell cancer (RCC) cell lines to raise monoclonal antibodies (mAbs) reactive with globo-series GSLs specifically expressed in RCCs. Although a number of mAbs reactive with globo-series GSLs were generated, they reacted with both RCC cell lines and normal kidney cells. When we analyzed recognized antigens by mAbs that were specifically reactive with RCC, but not with normal kidney cells at least on the cell surface, many of them turned out to be reactive with sulfoglycolipids. Eight out of 11 RCC-specific mAbs were reactive with SM2 alone, and the other 3 mAbs were more broadly reactive with sulfated glycolipids, i.e. SM3 and SM4 as well as SM2. In the immunohistochemistry, these anti-sulfoglycolipids mAbs showed RCC-specific reaction, with no or minimal reaction with adjacent normal tissues. Thus, immunization of A4galt KO mice with RCC-derived GSLs resulted in the generation of anti sulfated GSL mAbs, and these mAbs may be applicable for the therapeutics for RCC patients. 相似文献
88.
89.
Paul Micevych Clair B. Eckersell Krista Holland Akemi Smith 《Developmental neurobiology》1996,30(4):465-479
Estrogenic regulation of cholecystokinin (CCK) and its receptors is correlated with the initiation and termination of lordosis behavior. To understand the effect of circulating estrogen concentration on the temporal aspects of CCK mRNA expression in the posterodorsal medial amygdaloid nucleus (MeApd) and the central part of the medial preoptic nucleus (MPNc) of the limbic-hypothalamic circuit, ovariectomized female rats were treated with a 10 mm Silastic™ capsule filled with estradiol, a bolus injection of 50 μg estradiol benzoate or 2 μg estradiol benzoate every 4 days for five “cycles.” In situ hybridization was used to compare the relative changes of CCK mRNA levels at 0 h to levels measured at 6, 12, 24, 48, 72, or 96 h after estrogen administration. In the MPNc and the MeApd, the 10-mm capsule significantly increased and maintained CCK mRNA levels from 6 to 96 h. The range of the increase was 3.0–5.1-fold in the MPNc and 2.8–5.0 in the MeApd. The 50-μg injections significantly increased and maintained CCK mRNA levels in the MPNc from 12 to 96 h (range of the increase 2.4–4.1-fold) and in the MeApd from 24 to 96 h (range of the increase 2.2–2.8-fold). The repeated administration of 2 μg estrogen induced a significant increase of message levels in the MPNc at 12 and 24 h that were 4.2- and 4.7-fold, respectively. In the MeApd this estrogen treatment did not significantly increase CCK mRNA. These studies demonstrate that small doses (2 μg) of estrogen that mimic the pattern and circulating levels of estrogen dramatically stimulate CCK mRNA levels in the limbic-hypothalamic circuit. To further study this steroid stimulation, ovariectomized female rats were implanted with estradiol-filled cannulae into the bed nucleus of the stria terminalis or MeA. Estrogen elevated CCK mRNA levels locally in each nucleus. Implants in the bed nucleus also elevated CCK mRNA levels in the MeApd indicating that physiologic estrogen stimulation of CCK in the MeApd is the result of both local and distal transsynaptic elevation of CCK mRNA levels. The site-specific induction of CCK mRNA levels within the limbic-hypothalamic nuclei provides another important facet of estrogenic modulation of CCK induction. © 1996 John Wiley & Sons, Inc. 相似文献
90.
Hayakawa A Kawamoto Y Nakajima H Sakai J Takasawa R Nakashima I Magae J Tanuma S 《Apoptosis : an international journal on programmed cell death》2008,13(4):523-530
Vinorelbine is a chemotherapeutic vinca alkaloid clinically prescribed for non-small cell lung cancer and breast cancer. Here we studied the mechanism for vinorelbine-induced
apoptosis in a human T-cell lymphoma. Although vinorelbine induces DNA fragmentation that is inhibited by specific peptide
inhibitors for caspases-9 and -3 in Jurkat cells, caspase-8 deficiency retards vinorelbine-induced apoptosis. Activation of
caspase-8 is also observed in vinorelbine-treated cells, and the activity is diminished when the caspase-3 activity is blocked
by a specific peptide inhibitor, Ac-DNLC-CHO. Blocking of the Fas receptor with an antagonistic anti-Fas antibody does not
affect vinorelbine-induced DNA fragmentation. These results suggest that vinorelbine-induced apoptosis is enhanced by the
activation of caspase-8 via caspase-9-mediated activation of caspase-3, but not through a Fas-triggered signal. Western blotting
suggests that vinorelbine cleaves caspase-3, -9 and -8 and reduces the amount of mitochondrial cytochrome c. Caspase-8 deficiency suppresses all of these events. A downstream substrate for caspase-8, Bid, is also cleaved in vinorelbine-treated
cells, but the Bid truncation is also observed in caspase-8-deficient Jurkat cells. Importantly, recombinant caspases-3 and
-9, as well as caspase-8, directly cleaves recombinant Bid in vitro. These results suggest that caspases-3 and -9 participate
in Bid truncation, indicating a new mechanism for vinorelbine-induces apoptosis. 相似文献