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101.
Takayoshi Wakagi Eriko Fukuda Yoko Ogawa Hiroyasu Kino Hiroshi Matsuzawa 《FEBS letters》2002,510(3):196-200
An enzyme, which catalyzes both decarboxylation of indolepyruvate and subsequent oxidation of indoleacetaldehyde into indoleacetate, was purified from a thermoacidophilic archaeon, Sulfolobus sp. strain 7. The enzyme showed a Mr of 280 kDa on gel filtration and was composed of three subunits (a, 89; b, 30; and c, 19 kDa), possibly in a stoichiometry of 2:2:2. Mo and Fe were detected. Thiamine pyrophosphate was absent. Biotin was suggested to bind to the b-subunit. The first step, the decarboxylation reaction, was specific for 2-oxoacids with an aromatic group, while in the second reaction, various aldehydes including glyceraldehyde, which is a glycolytic intermediate in the organism, were oxidized. 相似文献
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N Pathan H Marusawa M Krajewska S Matsuzawa H Kim K Okada S Torii S Kitada S Krajewski K Welsh F Pio A Godzik J C Reed 《The Journal of biological chemistry》2001,276(34):32220-32229
Caspase-associated recruitment domains (CARDs) are protein interaction domains that participate in activation or suppression of CARD-carrying members of the caspase family of apoptosis-inducing proteases. A novel CARD-containing protein was identified that is overexpressed in some types of cancer and that binds and suppresses activation of procaspase-9, which we term TUCAN (tumor-up-regulated CARD-containing antagonist of caspase nine). The CARD domain of TUCAN selectively binds itself and procaspase-9. TUCAN interferes with binding of Apaf1 to procaspase-9 and suppresses caspase activation induced by the Apaf1 activator, cytochrome c. Overexpression of TUCAN in cells by stable or transient transfection inhibits apoptosis and caspase activation induced by Apaf1/caspase-9-dependent stimuli, including Bax, VP16, and staurosporine, but not by Apaf1/caspase-9-independent stimuli, Fas and granzyme B. High levels of endogenous TUCAN protein were detected in several tumor cell lines and in colon cancer specimens, correlating with shorter patient survival. Thus, TUCAN represents a new member of the CARD family that selectively suppresses apoptosis induced via the mitochondrial pathway for caspase activation. 相似文献
104.
I Takahashi H Kosaka K Oritani W R Heath J Ishikawa Y Okajima M Ogawa S Kawamoto M Yamada H Azukizawa S Itami K Yoshikawa Y Tomiyama Y Matsuzawa 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(6):3156-3163
A novel IFN-like molecule, limitin, was recently identified and revealed to suppress B lymphopoiesis through the IFN-alphabeta receptor, although it lacked growth suppression on myeloid and erythroid progenitors. Here we have studied diverse effects of limitin on T lymphocytes and compared limitin with previously known IFNs. Like IFN-alpha and -beta, limitin modified immunity in the following responses. It suppressed mitogen- and Ag-induced T cell proliferation through inhibiting the responsiveness to exogenous IL-2 rather than suppressing the production of IL-2. In contrast, limitin enhanced cytotoxic T lymphocyte activity associated with the perforin-granzyme pathway. To evaluate the effect of limitin in vivo, a lethal graft-versus-host disease assay was established. Limitin-treatment of host mice resulted in the enhancement of graft-versus-host disease. Limitin did not influence thymocyte development either in fetal thymus organ cultures or in newborn mice injected with limitin-Ig, suggesting that limitin is distinguishable from IFN-alpha and -beta. From these findings, it can be speculated that the human homolog of limitin may be applicable for clinical usage because of its IFN-like activities with low adverse effects on, for example, T lymphopoiesis, erythropoiesis, and myelopoiesis. 相似文献
105.
Akane Meguro Keiko Fujita Hitoshi Kunoh Timothy L. W. Carver Ralph L. Nicholson 《Mycoscience》2001,42(2):201-209
The release of extracellular matrix (ECM) and the emergence of germ tubes from conidia ofBlumeria graminis were studied by light microscopy and micromanipulation. More prompt and frequent ECM release was confirmed on an artificial
hydrophobic substratum than on an artificial hydrophilic substratum. Conidia initially incubated on the hydrophilic substratum
were transferred by micromanipulation to either the hydrophobic or the hydrophilic substrata. Immediately after transfer onto
the hydrophobic substratum, 75% of conidia released ECM, whereas only 16% did so upon transfer to the hydrophilic substratum.
Conidia transferred onto the hydrophobic substratum produced a primary germ tube (PGT) more promptly and frequently than those
transferred to the hydrophilic substratum. Thus, conidia recognize and respond to substratum hydrophobicity perhaps immediately
after contact. When inoculated onto either isolated barley cuticle or the hydrophobic artificial substratum, 2/3 of the conidia
produced a PGT from their polar regions. By contrast, on the hydrophilic substratum 2/3 of conidia did so from the side region.
These results show that substratum hydrophobicity affects the location of PGT emergence from conidia. Furthermore, the study
indicates that very rapid recognition of surface hydrophobicity by conidia promotes ECM release and this in turn may influence
the location of PGT emergence. 相似文献
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107.
Stereochemical properties of the glycosidic linkage have been studied by the quantum-chemical PCILO method, using 2-methoxytetrahydropyran as a model. Calculations of the two-dimensional, conformational (Φ, Ψ) maps showed that the rotation around the C-1---O-1 bond is more hindered than that around the O-1---C-6 bond, and that there are differences in the shape of the energy curve for the axial and equatorial forms of 2-methoxytetrahydropyran. The observed population of the five stable conformers at equilibrium (GG:GT:TG1:TG2:TT = 70.8:6.0:19.9:2.0:1.3) is consistent with the prediction of the anomeric and exo-anomeric effects. The calculated abundance (76.8%) of the axial form of 2-methoxytetrahydropyran is comparable with experimental results (77–80%) obtained by n.m.r. measurements in non-polar solvents. The energies found for individual conformers made it possible to calculate the magnitude of the anomeric effect (3 kJ/mol) and to determine, for the first time, the values of the exo-anomeric effect for axial (6 kJ/mol) and equatorial 2-methoxytetrahydropyran (7 kJ/mol). The calculated variations of the geometry arising from rotation around the C-1---O-1 bond are consistent with results obtained by statistical analysis of experimental data for - and β-glycosides. The results obtained, indicating that the energy, geometry, and electronic structure of glycosides are largely affected by the conformation of the acetal segment, are discussed from the point of view of conformational analysis of oligo- and poly-saccharides. 相似文献
108.
109.
110.
Toby Passioura Bhaskar Bhushan Anthony Tumber Akane Kawamura Hiroaki Suga 《Bioorganic & medicinal chemistry》2018,26(6):1225-1231
The combination of genetic code reprogramming and mRNA display is a powerful approach for the identification of macrocyclic peptides with high affinities to a target of interest. We have previously used such an approach to identify a potent inhibitor (CP2) of the human KDM4A and KDM4C lysine demethylases; important regulators of gene expression. In the present study, we have used genetic code reprogramming to synthesise very high diversity focused libraries (>1012 compounds) based on CP2 and, through affinity screening, used these to delineate the structure activity relationship of CP2 binding to KDM4A. In the course of these experiments we identified a CP2 analogue (CP2f-7) with ~4-fold greater activity than CP2 in in vitro inhibition assays. This work will facilitate the development of more potent, selective inhibitors of lysine demethylases. 相似文献