利用恒河婴猴模型对肠道病毒71型传播感染特征的生物学分析

本文在前期工作基础上,进一步对肠道病毒71型(EV71)从恒河婴猴的感染个体向其他未感染个体传播的可能性及相关生物学特性做了初步分析.通过喷雾形式经呼吸道感染1~2月龄恒河婴猴(A组);在观察临床症状同时,于感染后第7天,取该组动物粪便处理后,将上清液以喷雾形式经呼吸道感染新的婴猴个体(B组),随后对该次代感染个体进行...     

基于Voronoi图的丹顶鹤巢址空间格局分析

依据1996年、2003—2007年扎龙保护区丹顶鹤巢址数据,利用Voronoi图理论,分析了巢址的空间分布特征,计算了其拓扑指数—Voronoi图面积。结果表明,丹顶鹤巢址空间分布具有明显的空间聚类特征。以扎龙保护区的2006年巢址、居民点、国道和铁路为对象,构成其Voronoi图,较好地说明了人类活动对丹顶鹤巢址的空间格局的影响,研究结果对丹顶鹤栖息地的保护将起到重要作用。     

Collection of marine microalgae at the A. V. Zhirmunsky institute of marine biology

This article presents information about a special collection of marine microalgae at the Institute of Marine Biology (Far East Branch of the Russian Academy of Sciences). A list of microalgae currently maintained in the collection is given, and the main directions of the work with them are identified.     

Increment of hFIX expression with endogenous intron 1 in Vitro

This paper probes into the feasibility of increasing expression level of hFIX gene with endogenous intron 1 sequence.hFIX minigene was obtained with middle sequence truncated intron 1 inserted into the relative site of hFIX cDNA,and plasmid vector pKG5i‘IX,retroviral vector G1NaCi‘IX were constructed.These vectors were transduced into target cells of PA317,C2C12,primary rabbit skin fibroblasts (RSF) and primary human skin fibroblasts (HSF).The expression level of mixed colonies are PA317/pKG5i‘IX,151 ng/10^6 cells/24h;PA317/G1NaCi‘IX,308 ng/10^6 cells/24 h;C2C12/G1NaCi‘IX,186 ng/10^6 cells/24 h;RSF/G1NaCi‘IX,1929 ng/10^6 cells/24 h;HSF/G1NaCi‘IX,1646 ng/10^6 cells/24 h.These results indicated that hFIX minigene with intron l is able to increase the expression level to about 3 times of that of hFIX cDNA.Meanwhile,in order to study the application of hFIX minigene in the retroviral-mediated gene transfer system and refrain from intron splicing during viral production,a retroviral vector G1NaCi‘IXR with reversely inserted hFIX minigene expression cassette was constructed.The expression level of reverse constructor in PA317 cells was 390 ng/10^6 cells/24 h with 79% of bioactivity.PCR detection of HT/G1NaCi‘IXR cells infected with PA317/G1NaCi‘IXR supernatant confirmed the existence of intron 1 sequence.These results suggested that expression vector with forward-inserted intronl-carrying hFIX expression cassette can be used in directed gene transfer,but when using the retroviral-mediated gene transfer system,reversely-inserted intronl-carrying hFIX expression cassette should be considered.     

The species composition,morphology, and seasonal distribution of diatoms of the genus <Emphasis Type="Italic">Attheya</Emphasis> West, 1860 from the Sea of Japan

Six species that belong to the diatom genus Attheya were found in the Russian waters of the Sea of Japan. A. cf. flexuosa is a new record for the seas of Russia. A. decora is recorded for the first time for the Sea of Japan. The peculiarities of the morphology of A. decora and A. cf. flexuosa are described and illustrated. The study of the seasonal distribution, abundance, and ecology of Attheya species in the northwestern Sea of Japan showed that A. longicornis and A. ussurensis were the most numerous and widespread species, reaching maximum densities (up to 1.5 × 10⁶ and 1.8 × 10⁴ cells/liter, respectively) in the spring, summer, and fall. A. cf. flexuosa and A. septentrionalis were rare and were found in low numbers in the winter and early spring. Based on the ultrastructure of the girdle bands, the form and number of chloroplasts, the presence/absence of rimoportula on the valve, and habitat features, we distinguished two groups of species of the genus Attheya from the Sea of Japan. The first group includes psammophytes A. decora, A. arenicola, and A. ussurensis; the second one comprises A. longicornis and A. cf. flexuosa epiphytic on other diatoms, as well as A. septentrionalis, which is found in the under-ice plankton and is able to attach to the underside of ice.     

康氏木霉纤维素酶系中C_x酶多分子型的分离纯化及某些性质

从康氐木霉(Trichoderma k(?)ningii)白色变异株As 3.4001的粗酶制剂中,获得了纤维素酶系中的一组C_x酶(C_(x1) C_(x2) C_(x3) C_(x4))。分离步骤包括Sephadex G-75凝胶过滤,DEAESephadex A-50离子交换层析,ConA-Sepharose亲合层析,SE-Sephadex C-50离子交换层析及聚丙烯酰胺凝胶电泳。C_(x1)与C_(x2)的分子量不同而所带电荷相同,它们的分子量各自为44,500和34,000。C_(x2)—C_(x4)的分子量相同而所带电荷不同。纯化的C_(x1)—C_(x4)经聚丙烯酰胺凝胶电泳鉴定为单带。比较它们对羧甲基纤维素钠(CMC-Na)的糖化力及液化力表明在作用方式的随机性上C_(x2)>C_(x3)>C_(x1)>C_(x4)。     

Development in culture of the diatomChaetoceros salsugineus from the Sea of Japan

The development of the diatomChaetoceros salsugineus from Amurskii Bay in the Sea of Japan was investigated in laboratory culture. It is shown that the intraspecific taxaC. salsugineus f.salsugineus, C. salsugineus f.curtus, andC. salsugineus f.solitarius correspond to particular stages in the development of this species in culture. Problems of the taxonomy and biology of this species are discussed. It is assumed that the life cycle ofC. salsugineus includes a benthic stage represented by resting cells.     

马铃薯糖转运蛋白基因的克隆及表达分析

植物SWEET基因家族是一类糖转运蛋白,在植物的生理活动和生长发育过程中发挥着重要功能。为了解马铃薯SWEET基因的相关信息,探究其在马铃薯不同组织以及在生物胁迫与非生物胁迫下的表达特性。该研究采用同源克隆技术从马铃薯‘青薯9号’中克隆了StSWEET5基因(GenBank登录号为MN295671),其CDS序列长度为717 bp,编码238个氨基酸。系统进化树分析结果表明,StSWEET5与番茄的氨基酸序列相似性最高(97.06%)。qRT-PCR分析表明:StSWEET5基因在马铃薯各组织(根、茎、叶、花、块茎、匍匐茎)中均有表达,且在花中的表达显著高于其他组织;糖胁迫下,StSWEET5基因在根、茎、叶中均有表达,尤其在根中的表达差异最为显著(P0.05)。在晚疫病菌(Phytophthora infestans)诱导后36 h时,表达量达到最高,随后急剧下调。推测StSWEET5基因参与了马铃薯糖胁迫以及响应了晚疫病诱导的过程。     

Evaluation of the prevalence and economic burden of adverse drug reactions presenting to the medical emergency department of a tertiary referral centre: a prospective study

Background  

Adverse drug reactions (ADRs) are now recognized as an important cause of hospital admissions, with a proportion ranging from 0.9–7.9%. They also constitute a significant economic burden. We thus aimed at determining the prevalence and the economic burden of ADRs presenting to Medical Emergency Department (ED) of a tertiary referral center in India     

疣柄牛肝菌属一中国新记录种

报道了采自内蒙古呼伦贝尔市的中国疣柄牛肝菌属1个新记录种,即假褐疣柄牛肝菌Leccinum pseudoscabrum(Kallenb.)utara.。主要特征为菌肉伤后变红到紫褐色,再到黑褐色,基部菌丝体手触后呈紫褐色;具泡状菌丝,呈栅栏状。比较和讨论了该种与相近种在伤后不同的变色反应及菌丝形态。研究标本存放于吉林农业大学菌物标本馆(HMJAU)。