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Microglial immune response is impaired against the neurotropic fungus Lomentospora prolificans
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Aize Pellon Andoni Ramirez‐Garcia Xabier Guruceaga Alazne Zabala Idoia Buldain Aitziber Antoran Juan Anguita Aitor Rementeria Carlos Matute Fernando L. Hernando 《Cellular microbiology》2018,20(8)
Lomentospora (Scedosporium) prolificans is an opportunistic pathogen capable of causing invasive infections in immunocompromised patients. The fungus is able to disseminate via the bloodstream finally arriving at the central nervous system producing neurological symptoms and, in many cases, patient death. In this context, microglial cells, which are the resident immune cells in the central nervous system, may play an important role in these infections. However, this aspect of anti‐L. prolificans immunity has been poorly researched to date. Thus, the interactions and activity of microglial cells against L. prolificans were analysed, and the results show that there was a remarkable impairment in their performance regarding phagocytosis, the development of oxidative burst, and in the production of pro‐inflammatory cytokines, compared with macrophages. Interestingly, L. prolificans displays great growth also when challenged with immune cells, even when inside them. We also proved that microglial phagocytosis of the fungus is highly dependent on mannose receptor and especially on dectin‐1. Taken together, these data provide evidence for an impaired microglial response against L. prolificans and contribute to understanding the pathobiology of its neurotropism. 相似文献
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Fernando L. Hernando Esther Calvo Jose A. Rodriguez Pedro L. Barea Aitor Rementeria Maria Jesus Sevilla Jose Ponton 《Mycopathologia》1996,134(1):1-6
The effect of germ tube induction on the antigenic variability in C. albicans was studied in strains from blood cultures (Group I) and superficial candidiasis (Group II). When compared by immunoblotting with a rabbit antiserum, antigenic extracts from Group I strains grown as blastospores showed a higher reactivity than that of Group II strains. Major bands in Group I strains (45–47, 33, 30 kDa) were continuously expressed through the subcultures in vitro but, with the exception of the 45 kDa band, the reactivity of all of them decreased or disappeared after the tenth subculture in Group II strains. The induction of the germ tubes produced the re-expression of the antigens lost during subculture in the yeast form, the effect being very clear in Group II strains. The re-expression by C. albicans germ tubes of antigens lost during subculture of blastospores in vitro and the higher reactivity shown by Group I strains grown in mycelial phase should be taken into consideration when a test to detect anti-C. albicans antibodies is to be developed.Abbreviations GYE
glucose-yeast extract agar 相似文献
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Jesús María Arizmendi Olatz Fresnedo Mercedes Martinez-Bilbao Aitor Alaña Juan Luís Serra 《Physiologia plantarum》1987,70(4):703-707
The effect of different inorganic nitrogen sources on the cellular levels of nitrite reductase (NiR. EC 1.7.7.1) activity has been studied in the filamentous non-N2 -fixing cyanobacterium Phormidium laminosum (strain OH-1-p.Cl,). Nitrate-grown cells gave the highest NiR in cell-free extracts [ca 165 nmol of nitrite reduced (mg protein)-1 min-], whereas no activity could be detected in extracts from ammonium-grown cells. The in vivo effect of ammonium on NiR was similar to that exerted by chloramphenicol, suggesting that de novo synthesis of protein was probably repressed by this ion. When ammonium was removed from the culture medium, a rapid increase of de novo synthetized NiR occurred, and the appearance of the enzyme was slightly stimulated by the presence in the medium of either nitrate or nitrite. However, remarkably high levels of NiR [around 1.2 μmol of nitrite reduced (mg protein) −1 min−1 ] could be routinely measured in nitrogen-deficient cells, indicating that the enzyme was ammonium-repressible rather than nitrate- or nitrite-inducible. 相似文献
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Francisco J. Rodríguez-Tovar Alfred Uchman Aitor Payros Xabier Orue-Etxebarria Estibaliz Apellaniz Eustoquio Molina 《Palaeogeography, Palaeoclimatology, Palaeoecology》2010,285(1-2):50-65
Ichnological analysis of the upper Ypresian–lower Lutetian interval at the Gorrondatxe section (W Pyrenees, N Spain), reveals a relationship between sea-level dynamics and the eco-sedimentary factors influencing trace fossil assemblages. The 600 m thick section of deep-sea turbiditic deposits contains 41 ichnospecies belonging to 28 ichnogenera, which are typical of the Nereites ichnofacies, and mostly of the Paleodictyon ichnosubfacies, suggesting deposition in a basin plain to fan-fringe setting. The trace fossil diversity and abundance fluctuate, irrespective of turbidite frequency. These ichnological features are strongly affected by trophic level changes related partly to sea-level dynamics according to the sequence stratigraphic interpretations for the studied section. Temperature, oxygenation and substrate changes are also considered as relevant factors. Increased ichnodiversity, particularly among graphoglyptids, coincides with moderate oligotrophy and stable ecological conditions. Eutrophisation, lowered oxygenation and drop of temperature, typical of low sea level, can reduce ichnodiversity. 相似文献
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Sergio Delgado Alfredo Herrero Aitor Galarza Asier Aldalur Nere Zorrozua Juan Arizaga 《Population Ecology》2021,63(3):238-246
The management of mixed municipal waste can have an impact on wildlife and ecosystems. Previous studies have investigated how opportunistic species like gulls can react very fast to new landfills; however, the impact of landfill closure on bird populations is less investigated. Yet, there is a need to understand how fast and to what extent, animal populations can be adapted to new scenarios where the waste will not be deposited in landfill sites anymore. The aim is to determine the influence of landfill closures on apparent survival of a resident Yellow-legged Gull (Larus michahellis) population, used as a model species showing short-distance foraging movements, and with a high dependence on local food subsidies. Complementarily, we built some basic population growth models in order to determine how potential changes in survival (before/after landfill closure) will impact on population growth rate. Using a data set of 4,437 Yellow-legged Gull chicks ringed in four colonies over a period of 13 years, we obtained evidence supporting that the apparent survival was affected by landfill closure, especially if the landfill was located within a buffer of 10 km around the colony. Landfill closure affected the survival of first-year gulls (with a mean decrease of ~ 0.5–0.36), but not of older birds. Consequently, we did not detect a remarkable effect of landfill closures on population growth rate, probably due to the lack of effect on adult survival rates except for one of the surveyed colonies, where we found an annual decline of 7%. 相似文献
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Edurne Baroja-Fernndez Aitor Zandueta-Criado Milagros Rodríguez-Lpez Takashi Akazawa Javier Pozueta-Romero 《FEBS letters》2000,480(2-3)
The intracellular localizations of ADPglucose pyrophosphatase (AGPPase) and ADPglucose pyrophosphorylase (AGPase) have been studied using protoplasts prepared from suspension-cultured cells of sycamore (Acer pseudoplatanus L.). Subcellular fractionation studies revealed that all the AGPPase present in the protoplasts is associated with amyloplasts, whereas more than 60% of AGPase is in the extraplastidial compartment. Immunoblots of amyloplast- and extraplastid-enriched extracts further confirmed that AGPase is located mainly outside the amyloplast. Experiments carried out to identify possible different isoforms of AGPPase in the amyloplast revealed the presence of soluble and starch granule-bound isoforms. We thus propose that ADPglucose levels linked to starch biosynthesis in sycamore cells are controlled by enzymatic reactions catalyzing the synthesis and breakdown of ADPglucose, which take place both inside and outside the amyloplast. 相似文献
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