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Detection of a Salmonella enterica Serovar California Strain Spreading in Spanish Feed Mills and Genetic Characterization with DNA Microarrays
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Juan Alvarez Steffen Porwollik Idoia Laconcha Vassilis Gisakis Ana Beln Vivanco Iratxe Gonzalez Susana Echenagusia Nieves Zabala Felisa Blackmer Michael McClelland Aitor Rementeria Javier Garaizar 《Applied microbiology》2003,69(12):7531-7534
We performed an epidemiological study on Salmonella isolated from raw plant-based feed in Spanish mills. Overall, 32 different Salmonella serovars were detected. Despite its rare occurrence in humans and animals, Salmonella enterica serovar California was found to be the predominant serovar in Spanish feed mills. Different typing techniques showed that isolates of this serovar were genetically closely related, and comparative genomic hybridization using microarray technology revealed 23 S. enterica serovar Typhimurium LT2 gene clusters that are absent from serovar California. 相似文献
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Andoni Ramirez-Garcia Beatriz Arteta Ana Abad-Diaz-de-Cerio Aize Pellon Aitziber Antoran Joana Marquez Aitor Rementeria Fernando L. Hernando 《PloS one》2013,8(1)
The dimorphic fungus Candida albicans is able to trigger a cytokine-mediated pro-inflammatory response that increases tumor cell adhesion to hepatic endothelium and metastasis. To check the intraspecific differences in this effect, we used an in vitro murine model of hepatic response against C. albicans, which made clear that tumor cells adhered more to endothelium incubated with blastoconidia, both live and killed, than germ tubes. This finding was related to the higher carbohydrate/protein ratio found in blastoconidia. In fact, destruction of mannose ligand residues on the cell surface by metaperiodate treatment significantly reduced tumor cell adhesion induced. Moreover, we also noticed that the effect of clinical strains was greater than that of the reference one. This finding could not be explained by the carbohydrate/protein data, but to explain these differences between strains, we analyzed the expression level of ten genes (ADH1, APE3, IDH2, ENO1, FBA1, ILV5, PDI1, PGK1, QCR2 and TUF1) that code for the proteins identified previously in a mannoprotein-enriched pro-metastatic fraction of C. albicans. The results corroborated that their expression was higher in clinical strains than the reference one. To confirm the importance of the mannoprotein fraction, we also demonstrate that blocking the mannose receptor decreases the effect of C. albicans and its mannoproteins, inhibiting IL-18 synthesis and tumor cell adhesion increase by around 60%. These findings could be the first step towards a new treatment for solid organ cancers based on the role of the mannose receptor in C. albicans-induced tumor progression and metastasis. 相似文献
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Characterization of a Strain of Fukuyoa paulensis (Dinophyceae) from the Western Mediterranean Sea
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Aitor Laza‐Martínez Helena David Pilar Riobó Irati Miguel Emma Orive 《The Journal of eukaryotic microbiology》2016,63(4):481-497
A single cell of the dinoflagellate genus Fukuyoa was isolated from the island of Formentera (Balearic Islands, west Mediterranean Sea), cultured, and characterized by morphological and molecular methods and toxin analyses. This is the first report of the Gambierdiscus lineage (genera Fukuyoa and Gambierdiscus) from the western Mediterranean Sea, which is cooler than its eastern basin. Molecular analyses revealed that the Mediterranean strain belongs to F. paulensis and that it bears LSU rDNA sequences identical to New Zealand, Australian, and Brazilian strains. It also shared an identical sequence of the more variable ITS‐rDNA with the Brazilian strain. Toxin analyses showed the presence of maitotoxin, 54‐deoxyCTX1B, and gambieric acid A. This is the first observation of the two latter compounds in a Fukuyoa strain. Therefore, both Gambierdiscus and Fukuyoa should be considered when as contributing to ciguatera fish poisoning. Different strains of Fukuyoa form a complex of morphologically cryptic lineages where F. paulensis stands as the most distantly related nominal species. The comparison of the ITS2 secondary structures revealed the absence of CBCs among strains. The study of the morphological and molecular traits depicted an unresolved taxonomic scenario impacted by the low strains sampling. 相似文献
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José Luis Tellería Álvaro Ramírez Aitor Galarza Roberto Carbonell Javier Pérez-Tris Tomás Santos 《Journal of Biogeography》2009,36(2):220-229
Aim The abundance distribution of organisms at regional scales is commonly interpreted as the result of spatial variation in habitat suitability. However, the possibility that geography itself may affect patterns of distribution has received less attention. For example, the abundance of wintering bird populations might be influenced by the cost of reaching areas located far away from the main migratory pathways. We studied the abundance distribution of three common migratory passerines (meadow pipits, Anthus pratensis; common chaffinches, Fringilla coelebs; and European robins, Erithacus rubecula) wintering in farmlands located in the 600‐km long Cantabrian coastal sector of northern Spain, roughly perpendicular to the west Pyrenean migratory pathway that drives European migrant birds into the Iberian Peninsula. Location The study area occupies a belt located between the Atlantic coast and the Cantabrian Mountains in northern Spain. Methods We counted wintering and breeding birds and measured the structure of vegetation and environmental variables (altitude, rainfall, temperature) in 68 farmlands distributed at different distances from the west Pyrenean migratory flyway. We also studied the distribution of birds ringed in central and northern Europe and recovered in the study area between October and February. Analyses were based on single univariate statistics (chi‐square tests), ordination by principal components analysis and multiple regression. Results Controlling for the effects of climate, vegetation structure and local abundance of breeding conspecifics, the winter abundance of all three species decreased with the distance from their main migratory route in the western Pyrenees. Such patterns fitted well to the observed distribution of ringing recoveries. Main conclusions Our results support a link between the movements of birds along the Pyrenean migratory pathway and their winter abundance in northern Spain. According to this view, the sectors located near the migratory pathway seem to be more easily occupied by migrants, supporting the idea that proximity to passage areas may explain the fine‐grain regional patterning of species abundance in wintering grounds. 相似文献
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The temperature dependence of C3 photosynthesis may be altered by the growth environment. The effects of long-term growth in elevated CO2 on photosynthesis temperature response have been investigated in wheat ( Triticum aestivum L.) grown in controlled chambers with 370 or 700 μmol mol−1 CO2 from sowing through to anthesis. Gas exchange was measured in flag leaves at ear emergence, and the parameters of a biochemical photosynthesis model were determined along with their temperature responses. Elevated CO2 slightly decreased the CO2 compensation point and increased the rate of respiration in the light and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Vcmax , although the latter effect was reversed at 15°C. With elevated CO2 , Jmax decreased in the 15–25°C temperature range and increased at 30 and 35°C. The temperature response (activation energy) of Vcmax and Jmax increased with growth in elevated CO2 . CO2 enrichment decreased the ribulose 1,5-bisphosphate (RuBP)-limited photosynthesis rates at lower temperatures and increased Rubisco- and RuBP-limited rates at higher temperatures. The results show that the photosynthesis temperature response is enhanced by growth in elevated CO2 . We conclude that if temperature acclimation and factors such as nutrients or water availability do not modify or negate this enhancement, the effects of future increases in air CO2 on photosynthetic electron transport and Rubisco kinetics may improve the photosynthetic response of wheat to global warming. 相似文献
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The bZIP-type transcription factor FlbB regulates distinct morphogenetic stages of colony formation in Aspergillus nidulans 总被引:1,自引:1,他引:0