Ultra-low Dose Aerosol Infection of Mice with Mycobacterium tuberculosis More Closely Models Human Tuberculosis

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Significance of Devonian-Carboniferous radiolarians from accretionary terranes of the New England orogen, eastern Australia

Radiolarians provide age constraints for many previously undated terranes in the New England Orogen (NEO), a tectonic collage developed along the eastern margin of Australia.Djungati terrane, the age range of which was previously unknown, contains two distinctive siliceous sedimentary lithofacies. The oldest is a thick sequence of red, ribbon-bedded cherts which probably accumulated in a deep ocean-floor setting far from land. Middle Silurian through Late Devonian radiolarians have been recovered from these cherts. Green tuffaceous cherts which contain a latest Devonian (Famennian) radiolarian fauna depositionally overlie the lower red ribbon-bedded chert sequence. These cherts are intercalated with volcaniclastic sediments and the fauna which they contain can be used to constrain the timing of accretion of older rocks into a subduction complex.Anaiwan terrane, which was also previously undated, contains thin ribbon-bedded cherts which are depositionally overlain by tuffaceous chert, siliceous siltstones and volcaniclastic sediments. Latest Devonian (?late Famennian) and Early Carboniferous radiolarians have been recovered from these cherts and tuffaceous siltstones.Radiolarians also occur in fine-grained siliceous sediments of the Yarrimie Formation, part of the Gamilaroi terrane. These radiolarians are of Late Devonian (Frasnian) affinity and their presence indicates that blocks of limestone, which contain Givetian conodonts and corals and were previously thought to indicate the age of the Yarrimie Formation, are allochthonous.     

A study of endometrial RNA polymerase activity in infertile women.

Endometrial RNA polymerase activity, which is sensitive to oestrogens and progesterone, has been measured in normal and infertile patients. One-third of the infertile patients studied had abnormal values. An analysis of the relationship of abnormal RNA polymerase activity to clinical abnormalities and to pregnancies occurring without treatment suggests that this assay may detect a biochemical fault in the endometrium which is related to the failure to conceive.     

A Role for the Karyopherin Kap123p in Microtubule Stability

Several components of the nuclear transport machinery play a role in mitotic spindle assembly in higher eukaryotes. To further investigate the role of this family of proteins in microtubule function, we screened for mutations in Saccharomyces cerevisiae that confer sensitivity to microtubule-destabilizing drugs. One mutant exhibiting this phenotype lacked the gene encoding the karyopherin Kap123p. Analysis of kap123 Δ cells revealed that the drug sensitivity was caused by a defect in microtubule stability and/or assembly. In support of this idea, we demonstrated genetic interactions between the kap123 Δ mutation and mutated alleles of genes encoding α-tubulins and factors controlling microtubule dynamics. Moreover, kap123 Δ cells exhibit defects in spindle structure and dynamics as well as nuclear positioning defects during mitosis. Cultures of kap123 Δ strains are enriched for mononucleated large-budded cells often containing short spindles and nuclei positioned away from the budneck, phenotypes indicative of defects in both cytoplasmic and nuclear microtubules. Finally, we identified a gene, CAJ1 , which when deleted in combination with KAP123 exacerbated the microtubule-related defects of the kap123 Δ mutants. We propose that Kap123p and Caj1p, a member of the Hsp40 family of proteins, together play an essential role in normal microtubule function.     

Allelic association between a Ser-9-Gly polymorphism in the dopamine D3 receptor gene and schizophrenia

We examined a Ser-9-Gly polymorphism in the dopamine D3 receptor gene for allelic association with schizophrenia in 133 patients currently treated with clozapine and 109 controls. Allele 1 (Ser-9) was significantly more frequent in the patients (69%) than in the controls (56%) (P = 0.004). The 1-1 genotype was more common (43% vs 30%) and the 2-2 genotype less common (5% vs 18%) in patients than in controls. When the patient group was subdivided on the basis of clinical response to clozapine, using a 20-point improvement in the global assessment scale as cut-off, genotype 1-1 was found to be more frequent among the non-responders (53% vs 36%, P = 0.04). To place our results in the context of previous studies of this polymorphism and schizophrenia, we performed a meta-analysis of all published data including the present sample. The combined analysis shows evidence for a modest association between genotype 1-1 and schizophrenia (odds ratio 1.25, 95% confidence interval 1.05– 1.49, P = 0.01). These results suggest that the Ser-9 allele, or a nearby polymorphism in linkage disequilibrium, results in a small increase in susceptibility to schizophrenia. Received: 21 August 1995 / Revised: 14 December 1995