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151.
The nucleotide sequences of the hly and plc A genes (encoding listeriolysin, a thiol-activated cytolysin and a non-specific phospholipase C, respectively) were determined for two strains of Listeria monocytogenes serotype 1/2b. The deduced amino acid sequences for listeriolysin were identical for the two strains and identical to that of listeriolysin from serotype 4b. The two serotype 1/2b strains differed in one amino acid (histidine vs /asparagine) in the deduced amino acid sequence for phosopholipase C. 相似文献
152.
Yongqian Zhao Tingjin Sherryl Soh Kitti Wing Ki Chan Sarah Suet Yin Fung Kunchithapadam Swaminathan Siew Pheng Lim Pei-Yong Shi Thomas Huber Julien Lescar Dahai Luo Subhash G. Vasudevan 《Journal of virology》2015,89(20):10717-10721
We examined the function of the conserved Val/Ile residue within the dengue virus NS5 interdomain linker (residues 263 to 272) by site-directed mutagenesis. Gly substitution or Gly/Pro insertion after the conserved residue increased the linker flexibility and created slightly attenuated viruses. In contrast, Pro substitution abolished virus replication by imposing rigidity in the linker and restricting NS5''s conformational plasticity. Our biochemical and reverse genetics experiments demonstrate that NS5 utilizes conformational regulation to achieve optimum viral replication. 相似文献
153.
Kumar Naveen Chaudhary Aishwarya Singh Deepali Teotia Sachin 《Journal of plant biochemistry and biotechnology.》2020,29(4):754-768
Journal of Plant Biochemistry and Biotechnology - Triacylglycerols (TAGs) are derived from ester linkage of fatty acids (FAs) and glycerol and stored by plants in their seeds as carbon and energy... 相似文献
154.
Sugunavathi Sepramaniam Arunmozhiarasi Armugam Kai Ying Lim Dwi Setyowati Karolina Priyadharshni Swaminathan Jun Rong Tan Kandiah Jeyaseelan 《The Journal of biological chemistry》2010,285(38):29223-29230
Aquaporins facilitate efficient diffusion of water across cellular membranes, and water homeostasis is critically important in conditions such as cerebral edema. Changes in aquaporin 1 and 4 expression in the brain are associated with cerebral edema, and the lack of water channel modulators is often highlighted. Here we present evidence of an endogenous modulator of aquaporin 1 and 4. We identify miR-320a as a potential modulator of aquaporin 1 and 4 and explore the possibility of using miR-320a to alter the expression of aquaporin 1 and 4 in normal and ischemic conditions. We show that precursor miR-320a can function as an inhibitor, whereas anti-miR-320a can act as an activator of aquaporin 1 and 4 expressions. We have also shown that anti-miR-320a could bring about a reduction of infarct volume in cerebral ischemia with a concomitant increase in aquaporins 1 and 4 mRNA and protein expression. 相似文献
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Almo SC Bonanno JB Sauder JM Emtage S Dilorenzo TP Malashkevich V Wasserman SR Swaminathan S Eswaramoorthy S Agarwal R Kumaran D Madegowda M Ragumani S Patskovsky Y Alvarado J Ramagopal UA Faber-Barata J Chance MR Sali A Fiser A Zhang ZY Lawrence DS Burley SK 《Journal of structural and functional genomics》2007,8(2-3):121-140
159.
The cell cytosol is crowded with macromolecules such as proteins, nucleic acids, and membranes. The consequences of such crowding remain unclear. How is the rate of a typical enzymatic reaction, involving a freely diffusing enzyme and substrate, affected by the presence of macromolecules of different sizes, shapes, and concentrations? Here, we mimic the cytosolic crowding in vitro, using dextrans and Ficolls, for the first time in a variety of sizes ranging from 15 to 500 kDa, in a concentration range 0–30% w/w. Alkaline phosphatase–catalyzed hydrolysis of p‐nitrophenyl phosphate (PNPP) was chosen as the model reaction. A pronounced decrease in the rate with increase in fractional volume occupancy of dextran is observed for larger dextrans (200 and 500 kDa) in contrast to smaller dextrans (15–70 kDa). Our results indicate that, at 20% w/w, smaller dextrans (15–70 kDa) reduce the initial rate moderately (1.4‐ to 2.4‐fold slowing), while larger dextrans (>200 kDa) slow the reaction considerably (>5‐fold). Ficolls (70 and 400 kDa) slow the reaction moderately (1.3‐ to 2.3‐fold). The influence of smaller dextrans was accounted by a combination of increase in viscosity as sensed by PNPP and a minor offsetting increase in enzyme activity due to crowding. Larger dextrans apparently reduce the frequency of enzyme substrate encounter. The reduced influence of Ficolls is attributed to their compact and quasispherical shape, much unlike the dextrans. © 2006 Wiley Periodicals, Inc. Biopolymers 83: 477–486, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com 相似文献
160.
Natarajan SR Heller ST Nam K Singh SB Scapin G Patel S Thompson JE Fitzgerald CE O'Keefe SJ 《Bioorganic & medicinal chemistry letters》2006,16(22):5809-5813
p38 inhibitors based on 3,4-dihydropyrido[4,3-d]pyrimidazin-2-one template were synthesized and their SAR explored. Benchmark compounds 30, 35, and 36 were found to be potent against the enzyme. Crystal structure of p38 in complex with 30 indicated a key pi-stacking interaction with the pendant tyrosine residue-35 in the glycine-rich loop. 相似文献